Toxicological aspects of<i>Campomanesia xanthocarpa</i>Berg. associated with its phytochemical profile

Sousa, Joubert Aires de; Prado, Lismare da Silva; Alderete, Bárbara Lopes; Boaretto, Fernanda Brião Menezes; Allgayer, Mariangela Costa; Miguel, Fabiano Moraes; Sousa, José da Silva; Marroni, Norma Possa; Lemes, Maria Luísa Brodt; Corrêa, Dione Silva; Ferraz, Alexandre de Barros Falcão; Picada, Jaqueline Nascimento

doi:10.1080/15287394.2018.1562392

Cited by 18 publications

(7 citation statements)

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“…Quantitative analysis showed a high content of polyphenolic compounds previously reported in guabiroba such as quercetin, gallic acid, kaempferol [35–38]. All these compounds are found in greater concentration in ripe fruits.…”

Section: Resultsmentioning

confidence: 79%

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpa Berg.) by comprehensive two‐dimensional liquid chromatography

et al. 2020

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This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two-dimensional liquid chromatography (LC × LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid, and acetonitrile) was used to optimize the extraction mixture for polyphenols from ripe and unripe guabiroba fruits. A quantitative LC × LC platform was proposed to characterize the guabiroba extracts using a RP-Amide column and a C18 column in the first and second dimensions, respectively. Antidiabetic properties, using in vitro enzyme assay models and in vivo antioxidant activity with the eukaryote model Saccharomyces cerevisiae, was measured. Total phenolics compounds were more efficiently extracted with 2% acetic acid solution and acetonitrile (50:50, v/v). A total of 37 different compounds were identified and quantified using the proposed LC × LC method (linearity ranging from 0.9990 to 0.9994, intra-and interday precision from 0.40 to 10.57% and, accuracy from 81.89 to 108.98%). Significant differences were observed between ripe and unripe guabiroba fruits, especially for the compounds geraldone and methyl galangin isomer. Guabiroba fruits showed significant antidiabetic and antioxidant properties and may be potentially adopted as part of dietary strategies in the management of early stages of type 2 diabetes and associated complications.

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Section: Resultsmentioning

confidence: 79%

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpa Berg.) by comprehensive two‐dimensional liquid chromatography

et al. 2020

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“…31 Due to the high chemical diversity produced by Campomanesia sp., the secondary metabolites concentrate several molecules with antioxidant effect. 32 In fact, the scavenger activity of C. reitziana leaf preparations against DPPH radical revealed that AECR can sequester this radical and that EAF scavenger this radical in a potent manner reaching a CI 50 equal to 0.65 µg/ml. Besides, HECR and DCMF also scavenged DPPH but reaching CI 50 values about 4 and 90 times higher than EAF, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…In line with our data, the presence of myricitrin in the leaves of C. xanthocarpa , C. guazumifolia , and C. pubescens was already detected chromatographically 31 . Due to the high chemical diversity produced by Campomanesia sp ., the secondary metabolites concentrate several molecules with antioxidant effect 32 . In fact, the scavenger activity of C. reitziana leaf preparations against DPPH radical revealed that AECR can sequester this radical and that EAF scavenger this radical in a potent manner reaching a CI 50 equal to 0.65 µg/ml.…”

Section: Discussionmentioning

confidence: 99%

“…One of the consequences of diarrhea is the softening of stools or an increase in the number of watery stools together with abdominal cramps 1‐40 . Castor oil has been a useful experimental tool in the search for antidiarrheal preparations 41 given that it is hydrolyzed in the duodenum by pancreatic enzymes, leading to the release of ricinoleic acid that promotes alteration of electrolytes permeability on the intestinal mucosa increasing peristaltic activity 41,42 .…”

Section: Discussionmentioning

confidence: 99%

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Preparations from Campomanesia reitziana reduce the gastrointestinal motility and castor oil‐induced diarrhea in a non‐opioid and non‐dopaminergic pathway in mice and display antimicrobial activity in vitro

Mariott

Mariano

Boeing

et al. 2021

Neurogastroenterology Motil

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Background This study investigated the antidiarrheal potential of the aqueous extract (AECR) and hydroalcoholic extract of Campomanesia reitziana leaves (HECR), its ethyl acetate (EAF) and dichloromethane fractions (DCMF), and myricitrin isolated from EAF. Methods The total phenols and flavonoids were measured, followed by chromatography and myricitrin isolation. The 2,2‐diphenyl‐1‐picryl‐hydrazyl scavenger activity, the cytotoxicity, and the effects on LPS‐induced nitrite production in intestinal epithelial cells (IEC‐6) were quantified. The effect of HECR, EAF, DCMF, and AECR on intestinal motility (IT), gastric emptying (GE), and castor oil‐induced diarrhea in mice was determined, as well as its antimicrobial activity. Key Results The administration of AECR 10% (10 ml/kg, p.o), but not HECR (300 mg/kg), reduced the GE and IT by 52 and 51%. The EAF and DCMF at 300 mg/kg also reduced IT but did not change GE. Moreover, AECR and EAF, but not DCMF, inhibited the castor oil‐induced diarrhea and naloxone or metoclopramide pretreatment did not change these effects. Myricitrin did not change IT and the evacuation index of mice. Finally, the dry residue of AECR inhibited bacterial growth and EAF showed bacteriostatic activity against S. aureus, E. coli, and S. typhimurium and antifungal for C. albicans. However, none of the preparations alter the viability of Giardia spp. trophozoites. Conclusions The AECR and EAF can be effective to treat diarrhea acting through opioid‐ or dopaminergic type 2 receptor‐independent mechanisms and by its antimicrobial actions.

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“…Comet assay was carried out to evaluate DNA damage following the recommendation made by Tice et al [39], with minor modi cations [40] in samples of blood and liver. The blood samples (50 µL) were transferred to heparin solution (Liquemine® 25000 UI, 10 µL) and samples of liver were transferred to cold PBS at pH 7.4.…”

Section: Alkaline Comet Assaymentioning

confidence: 99%

Melatonin Attenuates Inflammation, Oxidative Stress, and DNA Damage in Mice with Nonalcoholic Steatohepatitis Induced by a Methionine- and Choline-Deficient Diet

et al. 2022

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Nonalcoholic steatohepatitis (NASH) is a disease with a high incidence worldwide, but its diagnosis and treatment are poorly managed. In this study, NASH pathophysiology and DNA damage biomarkers were investigated in mice with NASH treated and untreated with melatonin (MLT). C57BL/6 mice were fed a methionine-and choline-de cient (MCD) diet for four weeks to develop NASH. Melatonin was administered at 20 mg/kg during the last two weeks. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured, and hepatic tissue was dissected for histological analysis, evaluation of lipoperoxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as nuclear factor-erythroid 2 (Nrf2), tumor necrosis factor alpha (TNF-α), inducible nitric oxide synthase (iNOS), and tumor necrosis factor alpha (TGF-β) expression by immunohistochemistry.DNA damage was evaluated using Comet assay, while a micronucleus test in bone marrow was performed to assess the genomic instability associated with the disease. Melatonin decreased AST and ALT, liver in ammatory processes, balloonization and brosis in mice with NASH, decreasing TNF-α, iNOS, and TGF-β, as well as oxidative stress, shown by reducing lipoperoxidation, and intensifying Nrf2 expression. The SOD and GPx activities were increased while CAT was decreased by treatment with MLT.Although the micronucleus frequency was not increased in mice with NASH, a protective effect on DNA was observed with MLT treatment in blood and liver tissues using Comet assay. As conclusions, MLT slows down the progression of NASH, reducing hepatic oxidative stress and in ammatory processes, inhibiting DNA damage via anti-in ammatory and antioxidant actions.

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Toxicological aspects ofCampomanesia xanthocarpaBerg. associated with its phytochemical profile

Cited by 18 publications

References 50 publications

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpa Berg.) by comprehensive two‐dimensional liquid chromatography

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpa Berg.) by comprehensive two‐dimensional liquid chromatography

Preparations from Campomanesia reitziana reduce the gastrointestinal motility and castor oil‐induced diarrhea in a non‐opioid and non‐dopaminergic pathway in mice and display antimicrobial activity in vitro

Melatonin Attenuates Inflammation, Oxidative Stress, and DNA Damage in Mice with Nonalcoholic Steatohepatitis Induced by a Methionine- and Choline-Deficient Diet

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