Toxicity of Methyl- and Halogen-Substituted Alcohols in Tissue Culture Relative to Structure–Activity Models and Acute Toxicity in Mice

Dillingham, Elwood O.; Mast, Richard; Bass, George E.; Autian, John

doi:10.1002/jps.2600620103

Cited by 54 publications

(9 citation statements)

References 22 publications

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“…In other words, the toxicity potential of the ester at a cellular level, whether in cell culture or in the intact animal, is related to the concentration of the agent in the aqueous environment in contact with the cells and the intrinsic toxicity of the compound. Although this simple empirical relation between in vivo and in vitro toxicity raises a number of basic questions relative to the nature and mechanism of expression of toxicity in the two systems, the authors have demonstrated a similar relation between in vivo and in vitro toxicity for a series of halogen-and methyl-substituted alcohols (28). Warren and Glick (29) reported that the specific activity of the membrane and particulate fractions of mouse fibroblasts in labeled medium increased at a rate independent of cell division.…”

Section: Tim5/ld50 = Constantmentioning

confidence: 91%

Toxicity and health threats of phthalate esters: review of the literature.

Autian¹

1973

Environ Health Perspect

307

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Section: Tim5/ld50 = Constantmentioning

confidence: 91%

Toxicity and health threats of phthalate esters: review of the literature.

Autian¹

1973

Environ Health Perspect

307

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“…Extrapolation of cell culture test results to in vivo situations must also be performed with due caution. Cell culture techniques have been reported as being predictive for in vivo test results 39, 40 . In vitro analysis provides a method of investigating cytotoxicity in a simplified system that minimizes the effect of cofounding variables 41.…”

Section: Discussionmentioning

confidence: 99%

“…Cell culture techniques have been reported as being predictive for in vivo test results. 39,40 In vitro analysis provides a method of investigating cytotoxicity in a simplified system that minimizes the effect of cofounding variables. 41 Within the limitations of this in vitro study it can be concluded that none of the materials in each group had any toxic effect on the cells in MTT assay and accelerated aging for 150 and 300 h had no effect on the biocompatibility of silicone elastomers tested.…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxicity of maxillofacial silicone elastomers: Effect of accelerated aging

et al. 2008

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The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L-929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc-shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05).

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“…Table 1 lists the compounds used in the characterization together with their solute descriptors, and the log k in the TTAB-MEEKC system. Moreover, the log P o/w values of each compound reported in the literature are also listed [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. From references [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Suitability Of the Ttab-meekc System To Estimate Log P O/w Vmentioning

confidence: 99%

Estimation of the octanol-water distribution coefficient of basic compounds by a cationic microemulsion electrokinetic chromatography system

et al. 2020

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The octanol-water partition coefficient (Po/w), or the octanol-water distribution coefficient (Do/w) for ionized compounds, is a key parameter in the drug development process. In a previous work, this parameter was estimated through the retention factor measurements in a sodium dodecyl sulfate (SDS) - microemulsion electrokinetic chromatography (MEEKC) system for acidic compounds. Nonetheless, when ionized basic compounds were analyzed, undesirable ion pairs were formed with the anionic surfactant and avoided a good estimation of log Do/w. For this reason, an alternative MEEKC system based on a cationic surfactant has been evaluated to estimate Po/w or Do/w of neutral compounds and ionized bases. To this end, it has been characterized through the solvation parameter model (SPM) and compared to the octanol-water partition system. Results pointed out that both systems show a similar partition behavior. Hence, the log Po/w of a set of neutral compounds has been successfully correlated against the logarithm of the retention factor (log k) determined in this MEEKC system. Then, the log Do/w of 6 model bases have been estimated at different pH values and they have been compared to data from the literature, determined by the reference shake-flask and potentiometric methods. Good agreement has been observed between the literature and the estimated values when the base is neutral or partially ionized (up to 99 % of ionization).

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Toxicity of Methyl- and Halogen-Substituted Alcohols in Tissue Culture Relative to Structure–Activity Models and Acute Toxicity in Mice

Cited by 54 publications

References 22 publications

Toxicity and health threats of phthalate esters: review of the literature.

Toxicity and health threats of phthalate esters: review of the literature.

In vitro cytotoxicity of maxillofacial silicone elastomers: Effect of accelerated aging

Estimation of the octanol-water distribution coefficient of basic compounds by a cationic microemulsion electrokinetic chromatography system

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