Toxicity of metals and organic chemicals evaluated with bioluminescence assays

Ren, Shijin; Frymier, Paul D.

doi:10.1016/j.chemosphere.2004.07.005

Cited by 53 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such bioluminescent assays were confirmed to be simple and reliable in the detection of toxic substances. [11][12][13] Our previous study proposed an assay system using freshly incubated V. fischeri cells, in which BLI reduction of the cells responded to the toxicity of test samples. 2,3,13) In this study, 10 types of single antifouling chemicals as well as 45 combinations composed of two each were evaluated in our assay system.…”

Section: Introductionmentioning

confidence: 99%

Remarkable Synergistic Effects in Antifouling Chemicals against Vibrio fischeri in a Bioluminescent Assay

Zhou

Okamura

Nagata

2006

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Remarkable Synergistic Effects in Antifouling Chemicals against Vibrio fischeri in a Bioluminescent Assay

Zhou

Okamura

Nagata

2006

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

show abstract

“…4,7) The sensitivity of BLI at longer incubation time, however, was more reduced and deviations of data became larger in comparison with those of shorter one, as realized from average CV values in Table 2. Thus, it seems to be more efficient to use the short-term and long-term assays by using BLI reduction for 30 min and growth inhibition for 8 hr of incubations, respectively.…”

Section: Evaluation Of Natural Seawater Samplesmentioning

confidence: 93%

“…[1][2][3][4] MicroTox assay, as representative one of short-term assay, has been accepted as a standard method by International Standard Organization (ISO) due to its remarkable sensitivity. 2,3) In addition to these shortterm methods, various assays with longer incubation time to detect the toxicities of new substances have also been proposed.…”

Section: Introductionmentioning

confidence: 99%

Applicability of Luminescent Assay Using Fresh Cells of Vibrio fischeri for Toxicity Evaluation

Zhou

Okamura

Nagata

2006

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Toxicities of antifouling chemicals and natural marine samples were evaluated by three assays, among which bioluminescence assay using freshly incubated Vibrio fischeri (V. fischeri) cells (NZ assay) and MicroTox were regarded as short-term assays, and growth inhibition assay was conducted as long-term assay. Short-term toxicity levels evaluated by NZ assay were in good agreement with those by MicroTox assay for all of the samples examined. Based on the EC 50 values of each chemical by respective assay, NZ assay showed prior reproducibility and similar levels of sensitivity when compared with those of MicroTox assay. On the other hand, growth inhibition assay showed lower sensitivity and reproducibility than NZ and MicroTox assays. Four kinds of antifouling chemicals, Irgarol 1051, Diuron, thiabendazole (TBDZ), and N-dichlorofluoromethylthio-N′ ′ ′ ′ ′,N′ ′ ′ ′ ′-dimethyl-Nphenylsulfamide (DCF), were detected to possess delayed toxicity from the judgments on the difference of short-term and long-term toxicities. Four out of 16 seawater samples collected in Japan showed remarkable toxicity in NZ assay, suggesting that they were contaminated by several types of antifouling chemicals. Considering time consumed, facility for operation, cost, and requirements, NZ assay was proved to be efficient for toxicity evaluations for artificial and natural samples.

show abstract

“…Bioluminescence intensity of luminous bacteria reflects various factors to solve a variety of problems [6]. Numerous applications of the bacterial bioassay are known [7][8][9][10][11][12][13]. The advantages of the bioluminescent assays are speed, sensitivity, simplicity, and availability of the devices for toxicity registration [14].…”

Section: Introductionmentioning

confidence: 99%

Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

2014

View full text Add to dashboard Cite

Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research influence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specific component of non-thermal nature.

show abstract

Toxicity of metals and organic chemicals evaluated with bioluminescence assays

Cited by 53 publications

References 35 publications

Remarkable Synergistic Effects in Antifouling Chemicals against Vibrio fischeri in a Bioluminescent Assay

Remarkable Synergistic Effects in Antifouling Chemicals against Vibrio fischeri in a Bioluminescent Assay

Applicability of Luminescent Assay Using Fresh Cells of Vibrio fischeri for Toxicity Evaluation

Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

Contact Info

Product

Resources

About