Toxicity of Mangifera Indica aqueous stem bark extract evaluated in drosophila melanogaster used as model organism

Pam, Dung; Etuh, Monday Alexander; Oyeniran, Oluwatosin Imoleayo; Mdekere, I.W.

doi:10.1016/j.pharma.2021.02.005

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…Raven Used to treat nervous diseases, edema, dysentery, nephritis, diarrhea, headache and orchitis [209] Extract at 800 mg/kg did not induce toxic effect on BALB/c mice for 28 days [209] Meningitis Mangifera indica Leaf decoction is used to treat hepatitis in Burkina Faso and Uganda. Used to treat gastrointestinal, respiratory and genitourinary diseases, burn, itch, fever, cough, scurvy and throat/mouth infection [ 15 , 16 , 210 , 211 ] Mangiferin induced antiviral activity and controlled HSV replication, Larvicidal effect against yellow fever vector [ 41 , 211 ] Aqueous stem bark extract induced adverse effect and decreased the survival rate and emergence of younger flies of drosophila melanogaster [210] Hepatitis, monkey pox, meningitis, COVID-19, yellow fever, poliomyelitis, small pox, lassa fever Maytenus senegalensis (Lam.) Exell Used to treat opportunistic infections in PLHIV, wound, chest pain, rheumatism.…”

Section: Discussionmentioning

confidence: 99%

Traditional medicinal plants used for treating emerging and re-emerging viral diseases in northern Nigeria

Abubakar

Kankara

Malami

et al. 2022

European Journal of Integrative Medicine

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Section: Discussionmentioning

confidence: 99%

Traditional medicinal plants used for treating emerging and re-emerging viral diseases in northern Nigeria

Abubakar

Kankara

Malami

et al. 2022

European Journal of Integrative Medicine

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“…After a 5 days fly exposure to DTG by ingestion method, the climbing performance of the exposed or control flies was evaluated using the technique of Adedara et al 28 . Fertility was assessed as described by Pam et al 23 . Four groups of sixty virgin flies (both sexes) each in 3 replicates were exposed to the test drugs by ingestion for 5 days.…”

Section: Study Area: This Research Work Was Carried Out In January Tomentioning

confidence: 99%

“…Exposure of Drosophila melanogaster to toxicants can occur through inhalation, injection or ingestion methods 18 . The administration method chosen for this investigation was to mix the test drug with a tasty substrate like sucrose or brewerʼs yeast-supplemented fly diet 22 to improve palatability 23 . This model organism has been successfully used to assess antiretroviral drug-induced genotoxicity 24 and antioxidant deficits 25 .…”

Section: Introductionmentioning

confidence: 99%

Dolutegravir Increased Mortality Rate, Climbing Deficits and Altered Biomarkers of Oxidative Injury in Harwich Drosophila melanogaster

Iorjiim¹,

Omale²,

Bagu³

et al. 2023

J. of Pharmacology and Toxicology

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Background and Objective: Dolutegravir (DTG) is safer compared to Efavirenz (EFV) as a component of antiretroviral combination therapy.However, recent studies suggested that DTG might be associated with oxidative stress toxicity in humans. This study intended to assess whether a non-target organism, Drosophila melanogaster, could replicate the DTG-induced oxidative stress and to determine the potential toxicity of a finished dolutegravir product because of possible environmental contamination. Materials and Methods: Flies (mixed sexes) were exposed to a series of ten DTG concentrations (0-50 mg per 10 g fly food) for a 7 days LD 50 determination, followed by a 28 days survival assay, then a 5 days treatment to evaluate the effects of the test drug on negative geotaxis, fertility and some antioxidant biomarkers. Also, in silico molecular docking was performed to predict the possible molecular interactions of Drosophila acetylcholinesterase (AChE) and glutathione-S-transferase (GST) enzymes with the test drug. Results: The LD 50 of DTG in D. melanogaster was 16.53 mg/10 g of food. The DTG showed a significant (p<0.05) reduction in 28 days survival and climbing ability as well as alterations in the levels of biochemical markers [total thiols (T-SH), AChE, GST, catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA)] of the exposed flies. The test drug interacted with similar amino acid residues with a significantly (p<0.05) higher molecular attraction for the active sites of GST but a lower (p>0.05) binding affinity for those of AChE compared to the respective natural substrates and standard inhibitors. Conclusion: Oral administration of DTG in D. melanogaster significantly increased mortality rate, climbing and antioxidant deficits through a competitive antagonism of GST and AChE activities. The DTG-induced oxidative stress was replicated in the flies. Thus, environmental contamination with dolutegravir sodium could adversely affect non-target organisms in an ecosystem.

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“…Total thiol content was measured according to Ellman's method as modified by Pam et al (2021). The total reaction mixture was 600µL of (510 μL 0.1 M PBS pH 7.4, 30 μL of 10mM DTNB and 25μL of sample).…”

Section: Determination Of Total Thiol Determinationmentioning

confidence: 99%

Modulatory role of dose-dependent quercetin supplemented diet on behavioral and anti-oxidant system in <i>Drosophila melanogaster</i> model

Olanrewaju,

Bayo-Olugbami,

Enya

et al. 2023

J. Afr. Assoc. Physiol. Sci

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Background: Quercetin is an abundant bio-flavonoid in foods having several biological activities including anti-oxidant properties. However, the toxicity of quercetin may limit its use. Therefore, screening a graded dose of quercetin may be an important step in its use as a dietary supplement. Hence, the effects of graded doses of quercetin on the survival, locomotive behavior and oxidant-antioxidant system in D. Melanogaster was investigated.Methods: A Survival assay was conducted by feeding 50 fruit flies per vial with 50-400 mg/g quercetin-diet for 14 days. For behavioral and biochemical studies, flies were divided into 4 vials (50 flies each) 0, 100, 200 and 300 mg/g quercetin-diet. Flies were treated with vehicle or quercetin for 7 days. Behavioral assessment of negative geotaxis was determined from the climbing activities of flies. Finally, flies were killed, homogenized and centrifuged for supernatant for the assay of Catalase (CAT), Superoxide dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and Total thiol.Results: Quercetin treated groups showed a dose-dependent significant decrease (p<0.001) in survival rate. Climbing activity was unaltered at 100 mg but significantly (p<0.05) reduced at 200 and 300mg quercetin. Total thiol was not significantly altered (p>0.05) across the groups, MDA was reduced (p<0.05) at 300 mg compared to control, CAT activity was significantly increased (p<0.05) at 100-200 mg compared to control while GSH and SOD at 100-200 mg of quercetin increased.Conclusion: Quercetin showed better antioxidant activity at lower doses and adversely affected the climbing behavior and survival rate at higher doses. Therefore, quercetin use should be dose modulated and used with caution.

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Toxicity of Mangifera Indica aqueous stem bark extract evaluated in drosophila melanogaster used as model organism

Cited by 6 publications

References 17 publications

Traditional medicinal plants used for treating emerging and re-emerging viral diseases in northern Nigeria

Traditional medicinal plants used for treating emerging and re-emerging viral diseases in northern Nigeria

Dolutegravir Increased Mortality Rate, Climbing Deficits and Altered Biomarkers of Oxidative Injury in Harwich Drosophila melanogaster

Modulatory role of dose-dependent quercetin supplemented diet on behavioral and anti-oxidant system in <i>Drosophila melanogaster</i> model

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