Toxicity of iron oxide nanoparticles: Size and coating effects

Abakumov, Maxim; Semkina, Alevtina S.; Skorikov, Alexander; Vishnevskiy, Daniil A.; Ivanova, Anna; Mironova, Elena; Давыдова, Г. А.; Majouga, Alexander G.; Chekhonin, V. P.

doi:10.1002/jbt.22225

Cited by 92 publications

(63 citation statements)

References 22 publications

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“…Similarly, previous studies found correlations between cytotoxicity and the NPs’ hydrodynamic size [ 97 , 98 , 99 , 100 , 101 ]. In most cases, the hydrodynamic size of the NPs is larger than the core size.…”

Section: Resultssupporting

confidence: 71%

Predicting Cytotoxicity of Metal Oxide Nanoparticles Using Isalos Analytics Platform

et al. 2020

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A literature curated dataset containing 24 distinct metal oxide (MexOy) nanoparticles (NPs), including 15 physicochemical, structural and assay-related descriptors, was enriched with 62 atomistic computational descriptors and exploited to produce a robust and validated in silico model for prediction of NP cytotoxicity. The model can be used to predict the cytotoxicity (cell viability) of MexOy NPs based on the colorimetric lactate dehydrogenase (LDH) assay and the luminometric adenosine triphosphate (ATP) assay, both of which quantify irreversible cell membrane damage. Out of the 77 total descriptors used, 7 were identified as being significant for induction of cytotoxicity by MexOy NPs. These were NP core size, hydrodynamic size, assay type, exposure dose, the energy of the MexOy conduction band (EC), the coordination number of the metal atoms on the NP surface (Avg. C.N. Me atoms surface) and the average force vector surface normal component of all metal atoms (v⊥ Me atoms surface). The significance and effect of these descriptors is discussed to demonstrate their direct correlation with cytotoxicity. The produced model has been made publicly available by the Horizon 2020 (H2020) NanoSolveIT project and will be added to the project’s Integrated Approach to Testing and Assessment (IATA).

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Section: Resultssupporting

confidence: 71%

Predicting Cytotoxicity of Metal Oxide Nanoparticles Using Isalos Analytics Platform

et al. 2020

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“…However, there was no significant difference in bactericidal activity between macrophages with and without AMF application at the same MNP dose (Figure 5(B)), suggesting that exposure of MNPs to the RAW 264.7 macrophages was sufficient enough to exhibit a bactericidal activity against intracellular S. aureus. Since iron oxide magnetic nanoparticles can trigger the generation of ROS [33][34][35], and ROS can exhibit an antimicrobial activity [36], we next examined whether the improved bactericidal activity of macrophages with MNPs alone or MNP/ AMF hyperthermia was associated with increased ROS generation. The generation of intracellular ROS in the RAW 264.7 cells was significantly increased with increasing concentration of MNPs ( Figure 5(C)).…”

Section: Resultsmentioning

confidence: 99%

Mild magnetic nanoparticle hyperthermia enhances the susceptibility of Staphylococcus aureus biofilm to antibiotics

Alumutairi

filka

et al. 2020

International Journal of Hyperthermia

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Objective: A critical challenge in the treatment of biofilm infection is the capacity of biofilm-grown bacteria to develop resistance to traditional antimicrobial therapies. The objective of this study was to validate the therapeutic potential of magnetic nanoparticle/alternating magnetic field (MNP/AMF) hyperthermia in combination with conventional antibiotics against biofilm infection. Materials and methods: The impact of MNP/AMF hyperthermia on the viability of S. aureus biofilm in the absence and presence of antibiotics as well as on the bactericidal activity of macrophages were evaluated at varying conditions of MNPs concentration and AMF intensity using in vitro cell culture models. Results: The application of MNP/AMF alone at a CEM43 thermal dose below the threshold for skin tissue exhibited a modest efficacy in the eradication of Staphylococcus aureus (S. aureus) biofilm (<1-log reduction). The treatment of antibiotics (ciprofloxacin, vancomycin) alone at a bactericidal concentration for planktonic S. aureus had no significant effect on the eradication of biofilm phase of S. aureus. However, when the biofilm was pre-exposed to mild MNP/AMF hyperthermia, the treatment of antibiotics could exhibit bactericidal effects against S. aureus biofilm, which was associated with increased uptake of antibiotics to the bacterial cells. Importantly, the application of MNP/AMF could promote the bactericidal activity of macrophages against intracellular bacteria via MNP-dependent generation of reactive oxygen species (ROS). Conclusion: Our results validate that the application of mild MNP/AMF hyperthermia within a safe thermal dose threshold is synergistic with conventional antibiotics as well as aids host innate immune response of macrophages for the clearance of intracellular bacteria.

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“…, who verified the influence of IONPs coated with bovine serum albumin (BSA) as well as BSA‐coated and PEGylated on primary human fibroblasts and U251 human glioblastoma cell line. They found that BSA-coated and PEGylated IONPs had the less toxic influence on human fibroblasts and explained this effect by the protective action of PEG‐coating, which prevents the active uptake of NPs and delays their degradation and the release of iron ions 63 . Functionalization of our NPs with PEG might have caused that their negative impact on cell proliferation was usually less than this observed for Fe salts.…”

Section: Discussionmentioning

confidence: 99%

Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models

Janik-Olchawa

Dróżdż

Ryszawy

et al. 2020

Sci Rep

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In the paper, the results of the first regular studies of ultra-small iron oxide nanoparticles (IONPs) toxicity in vitro were presented. The influence of PEG-coated NPs with 5 nm magnetite core on six different cell lines was examined. These were: human bronchial fibroblasts, human embryonic kidney cells (HEK293T), two glioblastoma multiforme (GBM) cell lines as well as GBM cells isolated from a brain tumor of patient. Additionally, mouse macrophages were included in the study. The influence of IONPs in three different doses (1, 5 and 25 µg Fe/ml) on the viability, proliferation and migration activity of cells was assessed. Moreover, quantifying the intracellular ROS production, we determined the level of oxidative stress in cells exposed to IONPs. In the paper, for the first time, the effect of Fe in the form of IONPs was compared with the analogical data obtained for iron salts solutions containing the same amount of Fe, on the similar oxidation state. Our results clearly showed that the influence of iron on the living cells strongly depends not only on the used cell line, dose and exposure time but also on the form in which this element was administered to the culture. Notably, nanoparticles can stimulate the proliferation of some cell lines, including glioblastoma multiforme. Compared to Fe salts, they have a stronger negative impact on the viability of the cells tested. Ultra-small NPs, also, more often positively affect cell motility which seem to differ them from the NPs with larger core diameters.

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Toxicity of iron oxide nanoparticles: Size and coating effects

Cited by 92 publications

References 22 publications

Predicting Cytotoxicity of Metal Oxide Nanoparticles Using Isalos Analytics Platform

Predicting Cytotoxicity of Metal Oxide Nanoparticles Using Isalos Analytics Platform

Mild magnetic nanoparticle hyperthermia enhances the susceptibility of Staphylococcus aureus biofilm to antibiotics

Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models

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