Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models

Janik-Olchawa, Natalia; Dróżdż, Agnieszka; Ryszawy, Damian; Pudełek, Maciej; Planeta, Karolina; Setkowicz, Zuzanna; Śniegocki, Maciej; Ostachowicz, Beata; Chwiej, Joanna

doi:10.1038/s41598-020-72414-8

Cited by 11 publications

(4 citation statements)

References 76 publications

(73 reference statements)

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“…After 24 h of incubation, ca. 60% of cells survived at a dose of 5 μg/mL, but more than 90% of cells survived when treated with the three MIL-100 nanoMOFs. For MIL-100 Mw and MIL-100 RT , 59.8 and 34.5% of cell viability were observed at 20 μg/mL while, surprisingly, 97.9% of cell viability was preserved for MIL-100 60°C .…”

Section: Resultsmentioning

confidence: 98%

How Defects Impact the In Vitro Behavior of Iron Carboxylate MOF Nanoparticles

Ma,

Yu,

Nouar

et al. 2023

Chem. Mater.

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Nanoparticles of biocompatible iron carboxylate metal−organic frameworks (MOFs) or nanoMOFs are of great interest for biomedicine, in particular, for controlled drug release. However, little is known about the impact of their synthesis protocols on their defect content and the possible consequences in terms of physicochemical features and cytotoxicity. Here, we report for the first time how the defect content of the benchmark mesoporous iron trimesate MIL-100(Fe) (MIL stands for Materials of Institut Lavoisier) nanoparticles, with similar sizes but obtained from three different green synthesis routes, has a significant impact not only on their intrinsic porosity, stability in body fluids, drug loading capacity, and release but also shows a critical difference of in vitro toxicity and inflammatory response depending on the type of cell lines.

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Section: Resultsmentioning

confidence: 98%

How Defects Impact the In Vitro Behavior of Iron Carboxylate MOF Nanoparticles

Ma,

Yu,

Nouar

et al. 2023

Chem. Mater.

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“…Human glioblastoma multiforme T98G (ATCC, CRL-1690), U87MG (ATCC, HTB-14) and KJT23I (patient-derived) cells as well as human embryonic kidney HEK293T cells (ATCC, CRL-3216), primary human bronchial fibroblasts NHLF and mouse macrophages MAC (ATCC, CCL-46) were routinely cultivated in standard conditions with DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics: penicillin/streptomycin cocktail as it was described elsewhere 33 . For each experiment, cells were harvested with 0.25% Trypsin–EDTA solution (Gibco), counted in a Z2 particle counter (Beckman Coulter), and seeded at an appropriate density into tissue culture plates (Eppendorf).…”

Section: Methodsmentioning

confidence: 99%

The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models

Janik-Olchawa

Dróżdż

Ryszawy

et al. 2021

Sci Rep

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Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 µg Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages—being phagocytic cells—but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.

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“…To assess the cytotoxicity of different agents, cell viability tests are commonly used [ 6 , 7 ]. One of the most frequently applied viability assays is MTT, evaluating the NADH-dependent enzymatic activity [ 8 , 9 , 10 , 11 ]. The MTT assay is based on the ability of only viable cells to reduce and convert the relatively colorless MTT compound into formazan, which is a dark purple and a strongly lipophilic substance.…”

Section: Introductionmentioning

confidence: 99%

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

Dróżdż

Sławińska-Brych

Kubera

et al. 2022

IJMS

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4-(5-methyl-1,3,4-thiadiazole-2-yl) benzene-1,3-diol (C1) and 4-[5-(naphthalen-1-ylmethyl)-1,3,4-thiadiazol-2-yl] benzene1,3-diol (NTBD) are representative derivatives of the thiadiazole group, with a high antimycotic potential and minimal toxicity against normal human fibroblast cells. The present study has proved its ability to synergize with the antifungal activity of AmB. The aim of this work was to evaluate the cytotoxic effects of C1 or NTBD, alone or in combination with AmB, on human renal proximal tubule epithelial cells (RPTECs) in vitro. Cell viability was assessed with the MTT assay. Flow cytometry and spectrofluorimetric techniques were used to assess the type of cell death and production of reactive oxygen species (ROS), respectively. The ELISA assay was performed to measure the caspase-2, -3, and -9 activity. ATR-FTIR spectroscopy was used to evaluate biomolecular changes in RPTECs induced by the tested formulas. The combinations of C1/NTBD and AmB did not exert a strong inhibitory effect on the viability/growth of kidney cells, as evidenced by the negligible changes in the apoptotic/necrotic rate and caspase activity, compared to the control cells. Both NTBD and C1 displayed stronger anti-oxidant activity when combined with AmB. The relatively low nephrotoxicity of the thiadiazole derivative combinations and the protective activity against AmB-induced oxidative stress may indicate their potential use in the therapy of fungal infections.

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Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models

Cited by 11 publications

References 76 publications

How Defects Impact the In Vitro Behavior of Iron Carboxylate MOF Nanoparticles

How Defects Impact the In Vitro Behavior of Iron Carboxylate MOF Nanoparticles

The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

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