Toxicity of herbicides on Escherichia coli growth

Botelho, RG; Froes, CM; Santos, J.B.

doi:10.1590/s1519-69842012000100016

Cited by 21 publications

(11 citation statements)

References 17 publications

(22 reference statements)

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“…Strain Ros2 gave results 90 mg g -1 without stress and 110 mg g -1 with stress. Use of pesticides help in causing bacteriostatic effect on microorganisms (Botelho et al, 2012). According to the study, the maximum toxicity by pesticide pyriproxyfen was observed to nodule numbers in pea, root and shoot dry biomass, shoot nitrogen and root phosphorus in greengram, leghaemoglobin, seed protein and chlorophyll content in chickpea.…”

Section: Discussionmentioning

confidence: 95%

Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

2019

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Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

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Section: Discussionmentioning

confidence: 95%

Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

2019

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“…Toxicity of glyphosate to bacteria and other microorganisms have been reported (Botelho, et al, 2012;Shehata et al, 2013, Nweke et al, 2014. Glyphosate exhibits higher toxicity in soil-free media (Busse et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

Toxicity of Quaternary Mixtures of Phenolic Compounds and Formulated Glyphosate to Microbial Community of River Water

Nweke

Ike

Ibegbulem

2024

Ecotoxicol. Environ. Contam.

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The toxicity of mixtures of formulated glyphosate, phenol, 4-chlorophenol and 2,4-dichlorophenol to river water microbial community was investigated using non specific dehydrogenase activity as endpoint. The microbial community was exposed to individual toxicant and quaternary mixture ratios at concentrations ranging from 50 mg L -1 to 3000 mg L -1 over 24 h. The ecological doses (EC 50 ) as estimated from logistic or hormesis dose-effect models were 1317.946 ± 51.460 mg L -1 , 1046.414 ± 65.534 mg L -1 , 85.080 ± 4.468 mg L -1 and 60.897 ± 5.199 mg L -1 for formulated glyphosate, phenol, 4-chlorophenol and 2,4-dichlorophenol respectively. The toxicity and interaction of the mixtures were evaluated using concentration addition and toxic index models. The model deviation ratios (MDR) ranged from 0.553 ± 0.024 to 1.096 ± 0.021 while the toxic index (TI) varied between 0.912 ± 0.017 and 1.810 ± 0.078. However, the MDR and TI for all mixtures lie between 0.5 and 2.0. Thus, the joint action of the mixtures were considered additive.

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“…The in vitro evaluation of the toxic effects of herbicides revealed a negative effect on the growth of strains of E. coli , particularly at higher doses [34], and also served as a model for the identification of genes and enzymatic activities involved in the antioxidant system in general [48]–[50]. Until this date, there are no studies involving herbicides degrading ability by non-environmental strains as E. coli DH5-α.…”

Section: Resultsmentioning

confidence: 99%

“…Botelho et al [34] analyzed many widely used commercial formulations of herbicides, and only paraquat decreased the growth of the E. coli ATCC 25922 strain. In the present study, field doses (1×) of mesotrione, which is considered less toxic than the commercial herbicide [22], decreased the viability of E. coli DH5-α within 30 min.…”

Section: Resultsmentioning

confidence: 99%

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Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

et al. 2014

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The intensive use of agrochemicals has played an important role in increasing agricultural production. One of the impacts of agrochemical use has been changes in population structure of soil microbiota. The aim of this work was to analyze the adaptive strategies that bacteria use to overcome oxidative stress caused by mesotrione, which inhibits 4-hydroxyphenylpyruvate dioxygenase. We also examined antioxidative stress systems, saturation changes of lipid membranes, and the capacity of bacteria to degrade mesotrione. Escherichia coli DH5-á was chosen as a non-environmental strain, which is already a model bacterium for studying metabolism and adaptation. The results showed that this bacterium was able to tolerate high doses of the herbicide (10× field rate), and completely degraded mesotrione after 3 h of exposure, as determined by a High Performance Liquid Chromatography. Growth rates in the presence of mesotrione were lower than in the control, prior to the period of degradation, showing toxic effects of this herbicide on bacterial cells. Changes in the saturation of the membrane lipids reduced the damage caused by reactive oxygen species and possibly hindered the entry of xenobiotics in the cell, while activating glutathione-S-transferase enzyme in the antioxidant system and in the metabolizing process of the herbicide. Considering that E. coli DH5-α is a non-environmental strain and it had no previous contact with mesotrione, the defense system found in this strain could be considered non-specific. This bacterium system response may be a general adaptation mechanism by which bacterial strains resist to damage from the presence of herbicides in agricultural soils.

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Toxicity of herbicides on Escherichia coli growth

Cited by 21 publications

References 17 publications

Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

Toxicity of Quaternary Mixtures of Phenolic Compounds and Formulated Glyphosate to Microbial Community of River Water

Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

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