Toxicity of bioceramic and resinous endodontic sealers using an alternative animal model: Artemia salina

Malta, Cristiana Pereira; Barcelos, Raquel Cristine Silva; Segat, Hecson Jesser; Bürger, Marilise Escobar; Bier, Carlos Alexandre Souza; Morgental, Renata Dornelles

doi:10.4103/jcd.jcd_401_21

Cited by 7 publications

(17 citation statements)

References 26 publications

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“…These findings support the low cytotoxicity and good biocompatibility reported for these HCSB sealers. 15 , 16 , 18 , 35 , 36 , 37 , 38 , 40 , 41 BioC has lower toxicity than AHP in hPDLSCs 18 and exhibits similar toxicity to that of BC in a lethality assay involving brine shrimp, Artemia salina . 35 Furthermore, subcutaneously implanted BioC induces lower infiltration of inflammatory cells and IL-6 synthesis compared to AHP.…”

Section: Discussionmentioning

confidence: 96%

“… 15 , 16 , 18 , 35 , 36 , 37 , 38 , 40 , 41 BioC has lower toxicity than AHP in hPDLSCs 18 and exhibits similar toxicity to that of BC in a lethality assay involving brine shrimp, Artemia salina . 35 Furthermore, subcutaneously implanted BioC induces lower infiltration of inflammatory cells and IL-6 synthesis compared to AHP. 36 WST has been reported to be more biocompatible and less cytotoxic than AHP in human dental pulp stem cells, 37 human tooth germ stem cells, 37 hPDLSCs, 37 , 38 and MC3T3-E1, a typical osteoblastic cell line.…”

Section: Discussionmentioning

confidence: 96%

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Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Alchawoosh

Hashimoto

Kawashima

et al. 2023

Journal of Dental Sciences

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Alchawoosh

Hashimoto

Kawashima

et al. 2023

Journal of Dental Sciences

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“…[ 23 ] and Malta et al . [ 24 ] accredited the compatibility of the Totalfill BC sealer to the elution of the calcium ions and the proprietary additives in the bioceramic paste that play a vital role in the improved biocompatibility of the material.…”

Section: Discussionmentioning

confidence: 99%

“…[ 13 ] The AH Plus sealer showed favorable adaptability results due to its chemical bonding to root dentin, the sealer forms covalent bonds between the collagen of the dentin and epoxy resin. [ 24 ] When comparing the root thirds, AH plus and Totalfill sealers demonstrated larger gaps at the apical level compared to the coronal level. The reduced diameter as well as the density of the dentinal tubules at the apical region justifies the disparity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxicity and adaptability of a novel bioceramic root canal sealer: An in vitro and scanning electron microscope study

Emam,

Mahran,

Elshafei

2024

Journal of Conservative Dentistry and Endodontics

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Context: Cytotoxicity and adaptability are among the highly imperative tests that should be performed on a novel endodontic material to ensure its successful implementation in endodontic treatment. Aims: Assess a recently introduced bioceramic root canal sealer CeramoSeal with TotalFill BC and AH plus sealers regarding the cytotoxicity and adaptability. Materials and Methods: Five sealer discs were prepared for each sealer and their extracts were cultured in 96-well plates containing human fibroblasts for 24 h. After their incubation, MTT solution was added to each well plate using an enzyme-linked immunosorbent assay plate reader was implemented to calculate the percentage of viable cells. Thirty mandibular single-rooted premolars were prepared using the Edge Endo rotary system, teeth were divided into three groups (n = 10) based on the sealer type: Group 1 CeramoSeal, Group 2 Totalfill, and Group 3 AH plus sealer. Teeth were sectioned longitudinally and viewed under a scanning electron microscope where the region with the gaps was identified and quantified as a percentage of the root canal’s overall area. Statistical Analysis: One-way ANOVA test was used for cytotoxicity, while Kruskal–Wallis and Friedman’s tests were used for adaptability. Results: Ceramoseal statistically significantly showed the lowest viability, at high concentrations AH plus showed the highest cell viability, while at lower concentration Totalfill BC sealer showed the highest cell viability percentage. The gap percentages were statistically significantly higher in Ceramoseal group, there was no statistically significant difference between AH Plus and Totalfill groups. Conclusions: Ceramoseal sealer exhibited the lowest viability and highest gap percentage compared to the other sealers.

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“…By comparing the mean surviving larvae of the test and control, the percentage death was obtained. The best-fit line plotting concentration versus percentage lethality provides the 50% lethal concentration (LC 50 ) values (Malta et al, 2022).…”

Section: Brine Shrimp Lethality Assaymentioning

confidence: 99%

Potential effects of noni (Morinda citrifolia L.) fruits extract against obsessive-compulsive disorder in marble burying and nestlet shredding behavior mice models

Jeyabalan

Bala

Kavimani³

et al. 2022

Front. Pharmacol.

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Obsessive-compulsive disorder (OCD) is a chronic and complex psychiatric disorder that usually includes both obsessions and compulsions. Morinda citrifolia L. (Noni) is a functional food and it is a well-known plant due to its potential therapeutic effects on human health in many disorders including neurological and neurodegenerative diseases. The purpose of this study was to evaluate the potential effect of M. citrifolia fruits extract (MCFE) against obsessive-compulsive disorder using the marble burying and nestlet shredding behavior mice models. In addition, brain neurotransmitters such as dopamine (DA), serotonin and noradrenaline (NA) were also assessed. Five mice were placed in each of the different groups, and the treatment was given to the animals for a period of 15 days. The marble burying test was evaluated for 30 min on days 1, 7, and 14 while the nestlet shredding test was evaluated for 60 min on days 2, 8, and 15. Treatments with MCFE (100 and 200 mg/kg, p.o.) significantly improved in both behavior tasks when compared to the control group. In addition, diazepam (2 mg/kg, i.p.) and fluoxetine (15 mg/kg,

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Toxicity of bioceramic and resinous endodontic sealers using an alternative animal model: Artemia salina

Cited by 7 publications

References 26 publications

Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Evaluation of cytotoxicity and adaptability of a novel bioceramic root canal sealer: An in vitro and scanning electron microscope study

Potential effects of noni (Morinda citrifolia L.) fruits extract against obsessive-compulsive disorder in marble burying and nestlet shredding behavior mice models

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