Potential effects of noni (Morinda citrifolia L.) fruits extract against obsessive-compulsive disorder in marble burying and nestlet shredding behavior mice models

Abstract

Obsessive-compulsive disorder (OCD) is a chronic and complex psychiatric disorder that usually includes both obsessions and compulsions. Morinda citrifolia L. (Noni) is a functional food and it is a well-known plant due to its potential therapeutic effects on human health in many disorders including neurological and neurodegenerative diseases. The purpose of this study was to evaluate the potential effect of M. citrifolia fruits extract (MCFE) against obsessive-compulsive disorder using the marble burying and nestlet shredding behavior mice models. In addition, brain neurotransmitters such as dopamine (DA), serotonin and noradrenaline (NA) were also assessed. Five mice were placed in each of the different groups, and the treatment was given to the animals for a period of 15 days. The marble burying test was evaluated for 30 min on days 1, 7, and 14 while the nestlet shredding test was evaluated for 60 min on days 2, 8, and 15. Treatments with MCFE (100 and 200 mg/kg, p.o.) significantly improved in both behavior tasks when compared to the control group. In addition, diazepam (2 mg/kg, i.p.) and fluoxetine (15 mg/kg,

Identification of IGF-1 Effects on White Adipose Tissue and Hippocampus in Alzheimer’s Disease Mice via Transcriptomic and Cellular Analysis

“…Recent studies have suggested that maintaining a proper weight and stable glucose level can help prevent the progression of AD [ 65 , 66 ]. We conducted two types of behavior tests: the marble burying test, known as a test for anxiety, obsessive–compulsive behavior, and repetitive behaviors [ 67 , 68 ], and the nestlet shredding test, known as a test for motivation, psychiatric disorders [ 69 , 70 ], and dopamine-related depressive behaviors [ 71 ] ( Figure 1 D,E). Our data showed that the administration of IGF-1 improved both behavioral assessment scores in the APP/PS2 mice ( Figure 1 D,E).…”

“…The test polypropylene cages were kept in a separate room from the housing area. The number of marbles buried at least 2/3 of the way within 30 min was used to measure the compulsive-like digging behavior score [ 67 ]. A score was given when the mice had buried the marbles a distance of 2/3 from the surface into the bedding material [ 134 ].…”

“…Scoring was measured by the percentage of the nestlet that was shredded, which was calculated by dividing the weight of the remaining unshredded nestlet by the starting nestlet weight [ 134 ]. The weight of the shredded material (g) was calculated in a previous study: the weight of the shredded material (g) = the initial weight of the material—the weight of the material taken after the treatment [ 67 , 134 ].…”

The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

“…Nestlet shredding was measured between 12 and 13 mon post-IRR (Fig. 1A) as a complementary method of measuring compulsive-like behaviors (Jeyabalan et al . 2022).…”

“…Nestlet shredding was measured between 12 and 13 mon post-IRR (Fig. 1A) as a complementary method of measuring compulsive-like behaviors (Jeyabalan et al 2022). At 20:00, mice were singly housed into identical cages with standard chow and water ad libitum.…”