Toxicity evaluation of graphene oxide on cysts and three larval stages of Artemia salina

Zhu, Song; Luo, Fengguang; Chen, Weichao; Zhu, Bin; Wang, Gao‐Xue

doi:10.1016/j.scitotenv.2017.03.224

Cited by 51 publications

(34 citation statements)

References 33 publications

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“…43 In particular, in line with our observation, the few literature data on GO toxicity towards A. salina, limited to the larval stage, show an increased mortality of Artemia instar I nauplii only at GO concentrations higher than 100 μg mL −1 . [12][13][14] The resistance of larvae compared to adults is probably due to morphological and functional differences and metabolic states. For instance, instar I larvae lack of critical anatomical formations (e.g., mouth and anus), which start to develop at stage II, limiting the possibility of ingestion and the consequent bioaccumulation of GO.…”

Section: Discussionmentioning

confidence: 99%

“…47 Such effects were also recorded in A. salina and other organisms exposed to carbon-based nanomaterials, including GO, through the assessment of ROS production and the activity of antioxidant enzymes as biomarkers of oxidative stress. 12,13,48 The antioxidant enzyme considered in this work is GST, a multifunctional enzyme involved in the detoxification of xenobiotics and ROS, 49 also in Artemia. 50,51 The activity of detoxification enzymes can be altered in response to xenobiotics, making them suitable markers of stress caused by xenobiotics 52 and GST induction is part of an adaptive response mechanism to chemical stress, widely…”

Section: Discussionmentioning

confidence: 99%

“…ROS production in A. franciscana adults was evaluated after 24 and 72 h exposure to GO (1, 10 and 100 μg mL −1 ) using the 2′,7′-dichlorofluorescin diacetate (DCFDA) assay (Sigma-Aldrich; Milan, Italy) able to fluorimetrically measure hydroxyl/peroxynitrite radicals according to Zhu et al 13 Briefly, for each determination, 5 organisms were washed 3 times in TRIS-HCl (100 mM, pH 7.5) and homogenized in 1 mL of the same buffer for 15 seconds using an immersion sonicator (ultrasonic processor UP50H; Hielscher, Teltow, Germany). The samples were centrifuged at 12 000g for 15 min at 4°C, and the supernatant was collected and stored at −80°C.…”

Section: Ros Detectionmentioning

confidence: 99%

“…9 In addition, in vitro assays on mussel (Mytilus galloprovincialis) hemocytes showed that GO induced significant cytotoxic effects, with increased reactive oxygen species (ROS) production and membrane damage. 10 Moreover, studies carried out on crustaceans, such as Amphibalanus amphitrite larvae 11 and Artemia salina nauplii, [12][13][14] demonstrated the ability of GO to increase mortality and ROS production.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ecotoxicological impact of graphene oxide: toxic effects on the model organism Artemia franciscana

Cavion

Fusco

Sosa

et al. 2020

Environ. Sci.: Nano

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Ros Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ecotoxicological impact of graphene oxide: toxic effects on the model organism Artemia franciscana

Cavion

Fusco

Sosa

et al. 2020

Environ. Sci.: Nano

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“…From a toxicological viewpoint, nanomaterial size, shape, and surface properties, along with their chemical composition, are key features to consider for risk assessment (Nel et al 2006). Zhu et al (2017) reported oxidative damage and involvement of antioxidant enzymes in the larval stages of Artemia salina exposed to graphene oxide. Several nanomaterials have been shown to induce oxidative stress in organisms including marine bivalves (Manke et al 2013; Barbero and Yslas 2017).…”

Section: Introductionmentioning

confidence: 99%

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

Khan

Adeleye

Burgess

et al. 2019

Enviro Toxic and Chemistry

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Graphene is a 2‐dimensional nanomaterial with unique mechanical, thermal, electrical, and optical properties. With increasing applications of graphene‐family nanomaterials (GFNs) in electronics, biomedicine, and surface coatings, concern for their impacts on aquatic ecosystems is rising. Current information on the toxicity of GFNs, including graphene oxide, is scarce. Filter‐feeding bivalves, such as eastern oysters, are good models for nanomaterial exposure studies. We present results from a 72‐h static renewal oyster study using 1 and 10 mg/L graphene oxide, which, to our knowledge, is the first report on in vivo effects of graphene oxide exposures in marine bivalves. Water samples were analyzed for graphene oxide concentration and size assessments. Gill and digestive gland tissues were evaluated for lipid peroxidation and glutathione‐S‐transferase (GST) activity. In addition, gill sections were fixed for histopathological analyses. Elevated lipid peroxidation was noted in oysters exposed to 10 mg/L graphene oxide. No significant changes in GST activity were observed, but reduced total protein levels were found in digestive gland tissues of exposed oysters at both concentrations. Loss of mucous cells, hemocytic infiltration, and vacuolation were observed in gills of exposed oysters. The results indicate that short‐term graphene oxide exposures can induce oxidative stress and epithelial inflammation and adversely affect overall oyster health. Further investigations regarding the fate and sublethal effects of graphene oxide are critical to understanding the risks associated with a rapidly growing graphene consumer market. Environ Toxicol Chem 2019;38:820–830. Published 2019 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

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Toward the Application of Graphene for Combating Marine Biofouling

Jin

Tian

Bing

et al. 2020

Advanced Sustainable Systems

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The discovery of graphene has brought great innovations to materials science. Since tributyltin antifouling coatings were banned worldwide in 2008, the development of green antifouling coatings has become necessary. Recently, novel graphene‐related antifouling coatings have been the focus of many studies due to their enhanced mechanical strength, excellent antifouling capabilities, and environmental friendliness. This review starts by introducing the basic antifouling mechanisms of graphene nanosheets and the possible antifouling mechanisms when graphene is incorporated into coatings. Subsequently, the progress and existing problems regarding the application of graphene in the prevention of marine biofouling are discussed. Specifically, this review focuses on six graphene‐related antifouling coatings, including pure graphene film, nanohybrids, foul release coatings, photocatalytic nanocomposites, desalination membranes, and materials for uranium enrichment. The fabrication, antifouling properties, working mechanisms, and future development of graphene‐related antifouling coatings are highlighted. In addition, the potential environmental risk of using graphene materials in preventing marine biofouling is explained, and the basic requirements for designing an environmentally friendly graphene‐related antifouling coating are discussed. Finally, the prospect of applying graphene materials for combating marine biofouling is presented. This review aims to aid in improving the design of graphene‐related green antifouling coatings.

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Toxicity evaluation of graphene oxide on cysts and three larval stages of Artemia salina

Cited by 51 publications

References 33 publications

Ecotoxicological impact of graphene oxide: toxic effects on the model organism Artemia franciscana

Ecotoxicological impact of graphene oxide: toxic effects on the model organism Artemia franciscana

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

Toward the Application of Graphene for Combating Marine Biofouling

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