Toxicity effects of captan on different life stages of zebrafish (Danio rerio)

Zhou, Yanmin; Chen, Xiangguang; Teng, Miaomiao; Zhang, Jie; Wang, Chengju

doi:10.1016/j.etap.2019.04.003

Cited by 25 publications

(11 citation statements)

References 24 publications

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“…The concentrations of pesticides tested across experiments were: 0.1, 0.5, 1, 10, 100, 1.000 and 10.000 µg/L. These concentrations were chosen based on: i) the European drinking water directive (98/83/EC) and the ground water directive (2006/118/EC) which set a maximum concentration of 0.1 µg/L for an individual pesticide and 0.5 µg/L for total pesticides; ii) US aquatic life benchmarks for registered pesticides (https://www.epa.gov/pesticide-science-and-assessingpesticide-risks/aquatic-life-benchmarks-and-ecological-risk); iii) reported environmental concentrations (Morrissey et al, 2015;Qian et al, 2021Qian et al, , 2018Thompson et al, 2020;Velisek and Stara, 2018;Vu et al, 2016) and previous publications (Cao et al, 2019;Jia et al, 2020;Lulla et al, 2016;Osterauer and Köhler, 2008;Qian et al, 2021;Wang et al, 2020;Zhou et al, 2019). All water solutions were renovated every day.…”

Section: Zebrafish Pesticides Exposure Protocolmentioning

confidence: 99%

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

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Highlights 1) Perinatal pesticides links with long-term behavioral modifications in male mice.2) Behavioral changes are accompanied by electrographic brain slowing in vivo.3) In zebrafish larvae, pesticide cocktail elicited dose-dependent motor-behavioral changes.4) In zebrafish larvae, chlorpyriphos is a behavior-modifying pesticide contained in the cocktail.5) Non-lethal prenatal pesticide cocktail does not provoke brain malformations.

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Section: Zebrafish Pesticides Exposure Protocolmentioning

confidence: 99%

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

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“…Importantly, in addition to this antiviral activity, we demonstrated that P. phillyreifolius extract, as well as its major compound geraniin, do not show acute toxicity in vivo using a zebrafish model, relevant for drug toxicity assessment [43,44,[56][57][58]. Our zebrafish assay showed that neither the plant extract nor its major compound geraniin impact fish behavior (feeding and locomotion), and no sign of stress was reported during the 5 days of investigation following drug exposure.…”

Section: Discussionmentioning

confidence: 69%

The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish

et al. 2020

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The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV.

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“…It is of great interest to evaluate the toxicity of A. triplinervis major phytocompound THQ in vivo. Consequently, we took advantage of a newly established zebrafish model to test the biosafety of THQ extracted from A. triplinervis [35,37,38]. We noted that inoculation of THQ at the doses showing antiviral effect against ZIKV in vitro caused no obvious stress nor impaired locomotor and feeding behaviors in inoculated zebrafish.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, we aimed at exploring the innocuity of an acute exposure of an efficient antiviral concentration of THQ in vivo. For this, we took advantage of zebrafish as a relevant model for testing the toxicity of different compounds as previously shown in other studies [34,35,36,37]. Indeed, zebrafish is an interesting model sharing a high genomic homology with humans (>70%) as well as evolutionary conserved physiological processes, making it an interesting model for drug development and toxicity analysis [38,39].…”

Section: Introductionmentioning

confidence: 99%

Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish

Haddad

Picard

Bénard³

et al. 2019

Molecules

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Zika virus (ZIKV) is an emerging mosquito-borne virus of medical concern. ZIKV infection may represent a serious disease, causing neonatal microcephaly and neurological disorders. Nowadays, there is no approved antiviral against ZIKV. Several indigenous or endemic medicinal plants from Mascarene archipelago in Indian Ocean have been found able to inhibit ZIKV infection. The purpose of our study was to determine whether essential oil (EO) from Reunion Island medicinal plant Ayapana triplinervis, whose thymohydroquinone dimethyl ether (THQ) is the main component has the potential to prevent ZIKV infection in human cells. Virological assays were performed on human epithelial A549 cells infected with either GFP reporter ZIKV or epidemic viral strain. Zebrafish assay was employed to evaluate the acute toxicity of THQ in vivo. We showed that both EO and THQ inhibit ZIKV infection in human cells with IC50 values of 38 and 45 µg/mL, respectively. At the noncytotoxic concentrations, EO and THQ reduced virus progeny production by 3-log. Time-of-drug-addition assays revealed that THQ could act as viral entry inhibitor. At the antiviral effective concentration, THQ injection in zebrafish does not lead to any signs of stress and does not impact fish survival, demonstrating the absence of acute toxicity for THQ. From our data, we propose that THQ is a new potent antiviral phytocompound against ZIKV, supporting the potential use of medicinal plants from Reunion Island as a source of natural and safe antiviral substances against medically important mosquito-borne viruses.

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Toxicity effects of captan on different life stages of zebrafish (Danio rerio)

Cited by 25 publications

References 24 publications

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish

Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish

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