Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer, Isabel; Klément, Wendy; Gangarossa, Giuseppe; Zub, Emma; Bock, Frédéric de; Blaquière, Marine; Maurice, Tangui; Audinat, Etienne; Faucherre, Adèle; Lasserre, Frédèric; Ellero‐Simatos, Sandrine; Gamet-Payrastre, Laurence; Jopling, Chris; Marchi, Nicola

doi:10.1016/j.envpol.2021.116755

Cited by 10 publications

(6 citation statements)

References 91 publications

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“…The current findings revealed substantial overexpression of the GFAP gene in the TMX-intoxicated group. These findings are consistent with previous studies that found elevated GFAP expression to be a sensitive marker of neurotoxins such as imidacloprid and thiacloprid (Katić et al 2021 ; Forner-Piquer et al 2021 ; Abomosallam et al 2023 ). These changes may indicate higher dopaminergic and serotonergic turnover in the brain (Abd-Elhakim et al 2018 ).…”

Section: Discussionsupporting

confidence: 92%

The protective role of l-carnitine on oxidative stress, neurotransmitter perturbations, astrogliosis, and apoptosis induced by thiamethoxam in the brains of male rats

Abd Elkader,

Hussein,

Mohammed

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Synthetic organic insecticides such as pyrethroids, organophosphates, neonicotinoids, and others have the potential to disrupt ecosystems and are often toxic to humans. Thiamethoxam (TMX), a neonicotinoid insecticide , is a widely used insecticide with neurotoxic potential. l-Carnitine (LC) is regarded as the “gatekeeper” in charge of allowing long-chain fatty acids into cell mitochondria. LC is an endogenous chemical that is renowned for its prospective biological activity in addition to its role in energy metabolism. This study investigated the protective effects of LC against TMX-induced neurotoxicity in male Wistar rats. For 28 days, animals were divided into four groups and treated daily with either LC (300 mg/kg), TMX (100 mg/kg), or both at the aforementioned doses. Our results revealed marked serum lipid profile and electrolyte changes, declines in brain antioxidants and neurotransmitters (acetylcholine, dopamine, and serotonin levels) with elevations in thiobarbituric acid reactive substances and proinflammatory cytokine levels, as well as acetylcholinesterase and monoamine oxidase brain activity in TMX-treated rats. TMX also increased the expression of caspase-3 and glial fibrillary acidic protein. In contrast, pretreatment with LC attenuated TMX-induced brain injury by suppressing oxidative stress and proinflammatory cytokines and modulating neurotransmitter levels. It also ameliorated the expression of apoptotic and astrogliosis markers. It could be concluded that LC has antioxidant, anti-inflammatory, anti-astrogliosis, and anti-apoptotic potential against TMX neurotoxicity. Graphical abstract

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Section: Discussionsupporting

confidence: 92%

The protective role of l-carnitine on oxidative stress, neurotransmitter perturbations, astrogliosis, and apoptosis induced by thiamethoxam in the brains of male rats

Abd Elkader,

Hussein,

Mohammed

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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“…The total of animals used as controls was 1112, whereas the exposure individuals counted 2045. The highest concentration of fungicide in the meta-analysis was 20 mg/L for triadimefon 63 , while the lowest was 0.0001 mg/L for mepanipyrim, ziram, tiophanate, captan, and boscalid 26 , 64 .…”

Section: Resultsmentioning

confidence: 89%

Neurobehavioral effects of fungicides in zebrafish: a systematic review and meta-analysis

Reis,

Bastos,

Chitolina

et al. 2023

Sci Rep

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Pesticides are widely used in global agriculture to achieve high productivity levels. Among them, fungicides are specifically designed to inhibit fungal growth in crops and seeds. However, their application often results in environmental contamination, as these chemicals can persistently be detected in surface waters. This poses a potential threat to non-target organisms, including humans, that inhabit the affected ecosystems. In toxicologic research, the zebrafish (Danio rerio) is the most commonly used fish species to assess the potential effects of fungicide exposure, and numerous and sometimes conflicting findings have been reported. To address this, we conducted a systematic review and meta-analysis focusing on the neurobehavioral effects of fungicides in zebrafish. Our search encompassed three databases (PubMed, Scopus, and Web of Science), and the screening process followed predefined inclusion/exclusion criteria. We extracted qualitative and quantitative data, as well as assessed reporting quality, from 60 included studies. Meta-analyses were performed for the outcomes of distance traveled in larvae and adults and spontaneous movements in embryos. The results revealed a significant overall effect of fungicide exposure on distance, with a lower distance traveled in the exposed versus control group. No significant effect was observed for spontaneous movements. The overall heterogeneity was high for distance and moderate for spontaneous movements. The poor reporting practices in the field hindered a critical evaluation of the studies. Nevertheless, a sensitivity analysis did not identify any studies skewing the meta-analyses. This review underscores the necessity for better-designed and reported experiments in this field.

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“…The Y-maze apparatus had three identical arms (40 × 9 × 16 cm) placed at 120° with respect to each other. As previously described (Forner-Piquer et al, 2021), each mouse was placed at the end of one arm and allowed to explore freely the apparatus for 5 min. Spontaneous alternation performance (SAP) was assessed by scoring the pattern of entries into each arm during the 5 min of the test.…”

Section: Methodsmentioning

confidence: 99%

The gut-brain vagal axis scales hippocampal memory processes and plasticity

Onimus,

Arrivet,

de Oliveira Souza

et al. 2024

Preprint

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The vagus nerve serves as an interoceptive relay between the body and the brain. Despite its well-established role in feeding behaviors, energy metabolism, and cognitive functions, the intricate functional processes linking the vagus nerve to the hippocampus and its contribution to learning and memory dynamics remain still elusive. Here, we investigated whether and how the gut-brain vagal axis contributes to hippocampal learning and memory processes at behavioral, functional, cellular, and molecular levels. Our results indicate that the integrity of the vagal axis is essential for long-term recognition memories, while sparing other forms of memory. In addition, by combing multi-scale approaches, our findings show that the gut-brain vagal tone exerts a permissive role in scaling intracellular signaling events, gene expressions, hippocampal dendritic spines density as well as functional long-term plasticities (LTD and LTP). These results highlight the critical role of the gut-brain vagal axis in maintaining the spontaneous and homeostatic functions of hippocampal ensembles and in regulating their learning and memory functions. In conclusion, our study provides comprehensive insights into the multifaceted involvement of the gut-brain vagal axis in shaping time-dependent hippocampal learning and memory dynamics. Understanding the mechanisms underlying this interoceptive body-brain neuronal communication may pave the way for novel therapeutic approaches in conditions associated with cognitive decline, including neurodegenerative disorders.

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Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Cited by 10 publications

References 91 publications

The protective role of l-carnitine on oxidative stress, neurotransmitter perturbations, astrogliosis, and apoptosis induced by thiamethoxam in the brains of male rats

The protective role of l-carnitine on oxidative stress, neurotransmitter perturbations, astrogliosis, and apoptosis induced by thiamethoxam in the brains of male rats

Neurobehavioral effects of fungicides in zebrafish: a systematic review and meta-analysis

The gut-brain vagal axis scales hippocampal memory processes and plasticity

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