Toxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell lines

Abstract

There is a limited number of studies about the constituents of Hypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower and trunk were determined as eicosane, heptacosane, 2-propen-1-ol, hexahydrofarnesyl acetone and α-muurolene. HOE caused significant DNA damage at selected doses (250-750 µg/ml) while chromosomal damage was observed at higher concentrations (500 and 750 µg/ml). HOE demonstrated anti-growth activity in a dose-dependent manner between 3.13-100 µg/ml. Pyknotic nuclei were observed at 100 µg/ml concentration of HOE in both cell lines. In conclusion, HOE demonstrated cytotoxic effects in a cell type-dependent manner, however its genotoxic effects were observed at relatively higher doses.

Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

“…found the MN ‰ of negative control to be 13.10‰, 22 Balikci et al . found the MN ‰ of solvent control to be 24.50 ‰, 58 and Cobanoglu et al . found the MN ‰ of negative control to be 20.10‰ 59 .…”

“…According to the results, the micronucleus concentration (MN ‰) observed in negative control was 15.08 ± 0.45‰ (Table 1), which is compatible with some of the findings given in the literature. For example, Ari et al found the MN ‰ of negative control to be 13.10‰, 22 Balikci et al found the MN ‰ of solvent control to be 24.50 ‰, 58 and Cobanoglu et al found the MN ‰ of negative control to be 20.10‰. 59 It is also shown in Table 1 that the lymphocyte cultures treated with H 2 O 2 had 2.65-fold higher MN ‰ than the negative control (P < 0.05).…”

Natural Inhibitors of P-glycoprotein in Acute Myeloid Leukemia

“…There is little information regarding the biological properties of heptacosane, except those that report actions referable to phytocomplexes, for which it is one of the major constituents [ 110 , 111 , 112 , 113 ]. Heptacosane is one of the major components (8%) of the Centaurea baseri essential oil, Köse et al have previously described its multiple activities, including its cytotoxic properties against MCF-7, PANC-1, A549, and C6 glioma cell lines [ 114 ].…”

Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

“…olympicum and it has also given evidence about its cytotoxic activity on MCF-7 and MDA-MB-231 breast cancer cell lines [53]. Moreover, H. triquetrifolium methanol extract was shown to have a cytotoxic effect on different cell types including large cell lung carcinoma (COR-L23), hepatocellular carcinoma (Hep G2), renal adenocarcinoma (ACHN), amelanotic melanoma C32 cell lines [54].…”