Toxicity and cardiac effects of acute exposure to tryptophan metabolites on the kynurenine pathway in early developing zebrafish (Danio rerio) embryos

Majewski, Michał; Kasica, Natalia; Jakimiuk, A.; Podlasz, Piotr

doi:10.1016/j.taap.2018.01.004

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…Copper (Cu) is one of the most important microelements involved in energy metabolism, antioxidant defense, and the synthesis of neurotransmitters and neuropeptides. Cu is involved in tryptophan metabolism by regulating the activity of enzymes in the kynurenine pathway [1], which can generate toxic products when dysregulated [2]. Moreover, Cu is able to modulate systemic inflammation by inducing arachidonic acid conversion and prostanoid synthesis [3].…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

et al. 2020

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We aimed to study the physiological effects of diet supplemented with copper (Cu) nanoparticles (NPs). During the eight weeks of the experiment, young Wistar rats (at seven weeks of age, n = 9) were supplemented with 6.5 mg of Cu either as NPs or carbonate salt (Cu 6.5). A diet that was not supplemented with Cu served as a negative control (Cu 0). The impact of nano Cu supplementation on lipid (reflected as thiobarbituric acid reactive substances-TBARS) and protein peroxidation (thiol and carbonyl groups) in blood plasma as well as the influence on the vasodilatory mechanism(s) of isolated rat thoracic arteries were studied. Supplementation with Cu enhanced lipid peroxidation (TBARS) in NP 6.5 (x2.4) and in Cu 6.5 (x1.9) compared to the negative control. Significant increase in TBARS was also observed in NP 6.5 (x1.3) compared to the Cu 6.5 group. The level of thiol groups increased in NP 6.5 (x1.6) compared to Cu 6.5. Meanwhile, significant (x0.6) decrease was observed in the Cu 6.5 group compared to the negative control. Another marker of protein oxidation, carbonyl groups increased in NP 6.5 (x1.4) and Cu 6.5 (x2.3) compared to the negative control. However significant difference (x0.6) was observed between NP 6.5 and Cu 6.5. Arteries from Cu supplemented rats exhibited an enhanced vasodilation to gasotransmitters: nitric oxide (NO) and carbon monoxide (CO). An enhanced vasodilation to NO was reflected in the increased response to acetylcholine (ACh) and calcium ionophore A23187. The observed responses to ACh and CO releasing molecule (CORM-2) were more pronounced in NP 6.5. The activator of cGMP-dependent protein kinases (8-bromo-cGMP) induced similar vasodilation of thoracic arteries in NP 6.5 and Cu 0 groups, while an increased response was observed in the Cu 6.5 group. Preincubation with the inducible nitric oxide (iNOS) synthase inhibitor-1400W, decreased the ACh-induced vasodilation in NP 6.5 , exclusively. Meanwhile the eicosanoid metabolite of arachidonic acid (20-HETE) synthesis inhibitor-HET0016, enhanced vasodilation of arteries from Cu 0 group. In conclusion, this study demonstrates that supplementation with nano Cu influences oxidative stress, which further has modified the vascular response.

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Section: Introductionmentioning

confidence: 99%

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

et al. 2020

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“…In addition to these factors, the distance traveled by the zebrafish larvae was also related to the energy reserve provided by the yolk sac or yolk vesicle, which stores a nutrient substance (calf) responsible for providing the embryo with nutrients from the beginning of development until the start of exogenous feeding by the larva [ 60 ]. The loss of nutrients or failures in the absorption of nutrients from the yolk sac can result in decreased embryonic growth, due to decreased blood flow and cardiac changes [ 61 – 63 ]. Other changes observed include pericardial edema, yolk sac edema, opaque yolk, tail malformation, and bent spine.…”

Section: Resultsmentioning

confidence: 99%

Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture

et al. 2021

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Background Ascorbic acid (AA) is a micronutrient essential for the mechanisms of reproduction, growth, and defense in fish. However, the biosynthesis of this micronutrient does not occur in fish, so it must be supplied with food. A difficulty is that plain AA is unstable, due to the effects of light, high temperature, and oxygen, among others. The use of nanoencapsulation may provide protection and preserve the physicochemical characteristics of AA for extended periods of time, decreasing losses due to environmental factors. Method This study evaluated the protective effect of nanoencapsulation in polymeric nanoparticles (chitosan and polycaprolactone) against AA degradation. Evaluation was made of the physicochemical stability of the nanoformulations over time, as well as the toxicological effects in zebrafish (Danio rerio), considering behavior, development, and enzymatic activity. For the statistical tests, ANOVA (two-way, significance of p < 0.05) was used. Results Both nanoparticle formulations showed high encapsulation efficiency and good physicochemical stability during 90 days. Chitosan (CS) and polycaprolactone (PCL) nanoparticles loaded with AA had mean diameters of 314 and 303 nm and polydispersity indexes of 0.36 and 0.28, respectively. Both nanosystems provided protection against degradation of AA exposed to an oxidizing agent, compared to plain AA. Total degradation of AA was observed after 7, 20, and 480 min for plain AA, the CS nanoparticle formulation, and the PCL nanoparticle formulation, respectively. For zebrafish larvae, the LC50 values were 330.7, 57.4, and 179.6 mg/L for plain AA, the CS nanoparticle formulation, and the PCL nanoparticle formulation, respectively. In toxicity assays using AA at a concentration of 50 mg/L, both types of nanoparticles loaded with AA showed lower toxicity towards the development of the zebrafish, compared to plain AA at the same concentration. Although decreased activity of the enzyme acetylcholinesterase (AChE) did not affect the swimming behavior of zebrafish larvae in the groups evaluated, it may have been associated with the observed morphometric changes, such as curvature of the tail. Conclusions This study showed that the use of nanosystems is promising for fish nutritional supplementation in aquaculture. In particular, PCL nanoparticles loaded with AA seemed to be most promising, due to higher protection against AA degradation, as well as lower toxicity to zebrafish, compared to the chitosan nanoparticles. The use of nanotechnology opens new perspectives for aquaculture, enabling the reduction of feed nutrient losses, leading to faster fish growth and improved sustainability of this activity. Graphic Abstract

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“…Recent studies have indicated that intake of excessive doses of Cr (III) for a prolonged time period may lead to undesirable processes that modulate the immune response [ 9 , 10 , 11 ]. Chromium as picolinate (Cr–Pic) is an organic compound of Cr connected to picolinic acid, a tryptophan derivative on the kynurenine pathway [ 12 , 13 ], and is the most popular form available due to its higher absorption (2.8%) compared with inorganic forms (0.9%). Nonetheless, Cr–Pic is poorly absorbed; thus, other chemical compounds are being intensively studied to find a better preparation and to reduce the concentration of Cr.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, metal nanoparticles are of interest due to their small size, lack of electric charge, and specific physicochemical properties. On the other hand, these properties make nanoparticles a potential hazard for living organisms, as they easily penetrate biological membranes [ 5 , 10 , 11 , 12 , 13 , 14 , 15 ]. There is also a risk that Cr nanoparticles (Cr–NPs) increase oxidative processes in the body and that Cr (with no electric charge) can be transformed into the highly toxic Cr (VI).…”

Section: Introductionmentioning

confidence: 99%

Dietary Effects of Chromium Picolinate and Chromium Nanoparticles in Wistar Rats Fed with a High-Fat, Low-Fiber Diet: The Role of Fat Normalization

et al. 2022

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We aimed to evaluate how feeding a high-fat–low-fiber (F) diet to rats and dietary intervention with the implementation of a standard-fat-and-fiber (S) diet affects the response of the cardiovascular system to chromium (III) picolinate (Cr–Pic) and, alternatively, chromium nanoparticles (Cr–NPs). Young male Wistar Han rats (n/group = 12) from either the fatty group (18 weeks on F diet) or the intervention group (9 weeks on F diet + 9 weeks on S diet) received a pharmacologically relevant dose of 0.3 mg Cr/kg body weight in the form of Cr–Pic or Cr–NPs for 9 weeks. Our study on rats confirmed the pro-inflammatory effect of an F diet administered for 18 weeks. In the intervention group, both Cr–Pic and Cr–NPs decreased heart glutathione ratio (GSH+GSSG), enhanced participation of nitric oxide (NO) derived from inducible NO synthase (iNOS) in vascular relaxation to acetylcholine (ACh), increased the vasodilator net effect of cyclooxygenase-2 (COX-2)-derived prostanoids, and increased the production of superoxide anion (O2.−) in aortic rings. Meanwhile, in the fatty group, there was increased heart superoxide dismutase (SOD), decreased heart catalase (CAT), and reduced sensitivity in pre-incubated aortic rings to endogenous prostacyclin (PGI2). The factors that significantly differentiated Cr–NPs from Cr–Pic were (i) decreased blood antioxidant capacity of water-soluble compounds (0.75-fold, p = 0.0205), (ii) increased hydrogen peroxide (H2O2) production (1.59-fold, p = 0.0332), and (iii) modified vasodilator response due to PGI2 synthesis inhibition (in the intervention group) vs. modified ACh-induced vasodilator response due to (iv) COX inhibition and v) PGI2 synthesis inhibition with thromboxane receptor blockage after 18 weeks on F diet (in the fatty group). Our results show that supplementation with Cr–Pic rather than with Cr–NPs is more beneficial in rats who regularly consumed an F diet (e.g., for 18 weeks). On the contrary, in the intervention group (9 weeks on F diet + 9 weeks of dietary fat normalization (the S diet)), Cr–Pic and Cr–NPs could function as pro-oxidant agents, initiating free-radical reactions that led to oxidative stress.

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Toxicity and cardiac effects of acute exposure to tryptophan metabolites on the kynurenine pathway in early developing zebrafish (Danio rerio) embryos

Cited by 21 publications

References 31 publications

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture

Dietary Effects of Chromium Picolinate and Chromium Nanoparticles in Wistar Rats Fed with a High-Fat, Low-Fiber Diet: The Role of Fat Normalization

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