Toxic Compounds Analysis With High Performance Liquid Chromatography Detected By Electro Chemical Detector (Ecd)

Shintani, Hideharu

doi:10.19070/2167-910x-1400022

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Figure 1. The general structure of amanitin and a list of known amatoxins (Vetter, 1998) Several methods have been published for the isolation and analysis of amatoxins and phallotoxins, reverse-phased high-performance liquid chromatography (RP-HPLC) being the most common (Robinson-Fuentes et al, 2008;Garcia et al, 2015;Mcknight et al, 2015;Shintani, 2014;Yilmaz et al, 2014;Kaya et al, 2015;2013). Various analytical techniques, such as bioassays, radioimmunoassays, ultraviolet spectrometry, were formerly used.…”

Section: Introductionmentioning

confidence: 99%

Yüksek Çözünürlüklü Kütle Spektrometrenin Eşzamanlı Çoklu Mantar Toksin Tayinleri Üzerindeki Etkisi

Cevik¹

2020

Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Amatoxins are deadly wild mushroom toxins that cause severe poisoning in humans, from diarrhea to organ dysfunction. Mortality can be as high as 80% if no specific treatment is applied. In this study, separation and determination methods were developed for the simultaneous determination of 7 toxins belonging to Amanita phalloides. After extraction and purification of Amanita phalloides (death cap) wild mushrooms, toxins were detected using HPLC-ESI MS and exact mass with time of flight MS (TOF-MS) in positive ionization mode. In addition, it was observed that the toxins of α-and β-amanitine could be differentiated from each other thanks to HR-MS detection in case of their close retention (Rt) in LC. The presence of two toxins at the same retention point in the chromatogram was detected by differentiating the molecular ion mass (920.3514 DA) of the α-amanitine with the HR-MS (920.3696 DA). By comparing both optimized methods with each other, faster and broad-spectrum toxin identification was achieved compared to existing methods. In this study, the rapid and accurate identification of seven toxins simultaneously with the same method is of great importance. It will make important contributions to the treatment process, especially by determining that poisoning is caused by wild mushrooms.

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Section: Introductionmentioning

confidence: 99%

Yüksek Çözünürlüklü Kütle Spektrometrenin Eşzamanlı Çoklu Mantar Toksin Tayinleri Üzerindeki Etkisi

Cevik¹

2020

Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Environmental application of nanotechnology: air, soil, and water

Ibrahim

Hayyan

AlSaadi

et al. 2016

Environ Sci Pollut Res

306

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Global deterioration of water, soil, and atmosphere by the release of toxic chemicals from the ongoing anthropogenic activities is becoming a serious problem throughout the world. This poses numerous issues relevant to ecosystem and human health that intensify the application challenges of conventional treatment technologies. Therefore, this review sheds the light on the recent progresses in nanotechnology and its vital role to encompass the imperative demand to monitor and treat the emerging hazardous wastes with lower cost, less energy, as well as higher efficiency. Essentially, the key aspects of this account are to briefly outline the advantages of nanotechnology over conventional treatment technologies and to relevantly highlight the treatment applications of some nanomaterials (e.g., carbon-based nanoparticles, antibacterial nanoparticles, and metal oxide nanoparticles) in the following environments: (1) air (treatment of greenhouse gases, volatile organic compounds, and bioaerosols via adsorption, photocatalytic degradation, thermal decomposition, and air filtration processes), (2) soil (application of nanomaterials as amendment agents for phytoremediation processes and utilization of stabilizers to enhance their performance), and (3) water (removal of organic pollutants, heavy metals, pathogens through adsorption, membrane processes, photocatalysis, and disinfection processes).

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