Abstract. the use of true gas plasmas for the inactivation of microorganisms is an area of dynamic research. many types of gases are used as a source of plasma, and different plasma production methods have been applied. the antimicrobial mechanisms of oxygen-based gas plasmas may be due to an etching effect on microbial structures, particularly bacterial endospores resulting in shrinkage. By contrast, the definite mechanisms of actions of other gas plasma sources, such as n 2 , He, Ne, Ar and Xe gases, have not been clearly defined and indeed may be distinct. the speculated mechanisms of these gas plasmas involve the direct attack of metastable (excited molecular), UV and/or VUV to microbial structures, specifically the inner membrane and dna in the core of bacterial endospores. according to this speculation, sterilized spore figures would remain unchanged. However, these mechanisms remain to be clarified. Future perspectives on the use of gas plasma for sterilization are of interest, as it is possible that appropriate sterility assurance levels can be obtained in parallel with material and functional compatibility. Traditional sterilization methods are often limited in these requirements. therefore, gas plasma sterilization may prove to be an appropriate alternative sterilization procedure.
The plasma of several different gases has shown a sporicidal activity. From these gases, nitrogen gas was most difficult to produce atomic nitrogen radicals. However, these radicals have a high energy, indicating that nitrogen gas plasma could be used to sterilize microorganisms and inactivate endotoxins. The sterilization mechanism of nitrogen gas plasma is the synergistic effect of a high rising-up voltage pulse, UV irradiation and atomic nitrogen radicals. Thus, the target cells were damaged by degradation, which resulted in death. The biological indicator (BI) used in this study was Geobacillus stearothermophilus ATCC 7953 at a population of 1 x 106 CFU/sheet. Sterility assurance was confirmed by using the Bl. Moreover, endotoxins were successfully inactivated. More than 5 log reduction of endotoxins could be attained with 30 minutes of nitrogen gas plasma exposure. Material functionality influenced by nitrogen gas plasma presented a satisfactory result. No deterioration of polymers could be observed by nitrogen gas plasma exposure.
Ethylene oxide gas is an agent in the sterilization of medical devices due to its effectiveness and compatibility with most materials. The advantages and disadvantages, as well as its recommended uses, are explored in this review article. The variables and their relevance on process optimization are described, the types of processing cycles are detailed and emphasis is given to the design and validation of the sterilization process.
In this experiment, we found that a potential carcinogen, 4,4'-methylenedianiline (MDA), was produced from gamma-ray irradiated potting materials, medical thermosetting polyurethane (PU). gamma-Ray irradiated PUs were immersed in either methanol or in equine serum. The time course of MDA leached from the potting materials and other variables were compared. A significant relationship was noted between the amount extracted and the rigidity of the potting material. The accumulated amount of extracted MDA (a few ppm in the original sample) increased with increasing irradiation dose by a second-order equation. One sample indicated reduced MDA elution after irradiation in the early stage. MDA extraction with serum was 82-87% that with methanol. In one case, MDA leaching into serum in the early stage was higher than that into methanol. Autoclave sterilization did not promote MDA formation, thus indicating that autoclaving would be preferable.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.