Abstract:Non-thermal plasma research has put a growing focus on the bacteria inactivation problem. Here we show how non-thermal plasma destroys Gram-positive and Gram-negative bacteria and discuss the mechanisms of plasma bactericidal effects.
“…Importantly, air NTP produced elevated levels of NO, O 3 and in comparison with He NPT. Contrarily, in He NTP a substantial amount of He ions were detected, which were lacking in air NTP 5, 21 .Figure 1Physicochemical characterization of the chemically distinct non-thermal plasmasand cytotoxic effects elicited by different plasmas. ( a ) The fourier transform infrared spectroscopy (FT-IR) of air, helium NTPs and ozone.…”
Section: Resultsmentioning
confidence: 99%
“…A recent study shows that oxidants and their targets might be spatially confined within the cell 60 . Moreover, we have previously shown that air and He NTPs possess different ion penetration profiles 5, 21 . In addition, the ROS/RNS composition and concentrations range in the plasma-treated liquids are variable, depending on the carrier gas that forms NTP 9, 61 .…”
A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
“…Importantly, air NTP produced elevated levels of NO, O 3 and in comparison with He NPT. Contrarily, in He NTP a substantial amount of He ions were detected, which were lacking in air NTP 5, 21 .Figure 1Physicochemical characterization of the chemically distinct non-thermal plasmasand cytotoxic effects elicited by different plasmas. ( a ) The fourier transform infrared spectroscopy (FT-IR) of air, helium NTPs and ozone.…”
Section: Resultsmentioning
confidence: 99%
“…A recent study shows that oxidants and their targets might be spatially confined within the cell 60 . Moreover, we have previously shown that air and He NTPs possess different ion penetration profiles 5, 21 . In addition, the ROS/RNS composition and concentrations range in the plasma-treated liquids are variable, depending on the carrier gas that forms NTP 9, 61 .…”
A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
“…During the treatment time Δt = 1 min, the plasma produced a mean ion fluency of 6.4 · 10 14 ions cm −2 . Detailed characteristics of the system have been published previously102728.…”
Section: Methodsmentioning
confidence: 99%
“…We have previously developed and characterized NTP system with controlled plasma composition and working temperature that has been shown to be an effective tool for bacterial eradication2728. In our recent study we demonstrated that chemically distinct plasmas trigger different responses in mammalian cells, and that the extent of biological responses to NTP may grossly differ between phenotypically distinct cell lines27.…”
Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. We utilised a single jet NTP system generating atmospheric pressure air plasma, with ion volume density 5 · 1017 m−3 and gas temperature 30–35 °C. The skin wounds were exposed to three daily plasma treatments for 1 or 2 minutes and were evaluated 3, 7 and 14 days after the wounding by histological and gene expression analysis. NTP treatment significantly enhanced epithelization and wound contraction on day 7 when compared to the untreated wounds. Macrophage infiltration into the wound area was not affected by the NTP treatment. Gene expression analysis did not indicate an increased inflammatory reaction or a disruption of the wound healing process; transient enhancement of inflammatory marker upregulation was found after NTP treatment on day 7. In summary, NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.
“…Increasing evidence suggests that reactive oxygen species (ROS) are the major players in NTAPP-induced apoptosis in vitro 131415. However, there is a discrepancy between the cytotoxic effect of non-thermal plasma and ozone, which is a considerable component of non-thermal air plasma1617.…”
Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.
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