Towards the application of desorption electrospray ionisation mass spectrometry (DESI-MS) to the analysis of ancient proteins from artefacts

Heaton, Karl; Solazzo, Caroline; Collins, Matthew J.; Thomas‐Oates, Jane; Bergström, Edmund T.

doi:10.1016/j.jas.2009.05.016

Cited by 46 publications

(26 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DESI-MS, like DART-MS, allows the analysis of organic material with little or no sample preparation at ambient conditions (116). It has the capacity to detect and identify large molecules and, apart from proteins, has the potential to analyse triacylglycerols in fatty material, allowing the identification of fats without the need for isotopic analysis.…”

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

View full text Add to dashboard Cite

The analysis of organic residues from archaeological materials has become increasingly important to our understanding of ancient diet, trade and technology. Residues from diverse contexts have been retrieved and analysed from the remains of food, medicine and cosmetics to hafting material on stone arrowheads, pitch and tar from shipwrecks, and ancient manure from soils. Research has brought many advances in our understanding of archaeological, organic residues over the past two decades. Some have enabled very specific and detailed interpretations of materials preserved in the archaeological record. However there are still areas where we know very little, like the mechanisms at work during the formation and preservation of residues, and areas where each advance produces more questions rather than answers, as in the identification of degraded fats. This chapter will discuss some of the significant achievements in the field over the past decade and the ongoing challenges for research in this area.1341280_File000001_20903114.docPrinted 7/19/2013 2

show abstract

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

View full text Add to dashboard Cite

show abstract

“…), or the likelihood that blankets were traded over long distances. Nevertheless, the point here is not to suggest that Solazzo et al (2009) have provided a definitive answer to this particular issue, but rather to illustrate that useful biogeographic evidence can be obtained through archaeological protein residue analysis.…”

Section: Mountain Goats In Washingtonmentioning

confidence: 97%

“…The result of these interactions, despite not being completely understood, is that proteins have been recovered from a variety of contexts in which they are closely bound to a mineral surface. Schweitzer et al (2009), Heaton et al (2009) and Yohe et al (1991), to give a few examples, verify that proteins survive in fossilized bone, ancient ceramic artifacts, and groundstone implements. Although additional work is needed to fully evaluate the nature of protein-mineral interactions, these examples demonstrate that proteins bind to mineral matrices and that they can survive and be recovered after hundreds or even thousands of years.…”

Section: Protein Residuesmentioning

confidence: 99%

Archaeological Protein Residues: New Data for Conservation Science

Barker

2011

Ethnobiology Letters

View full text Add to dashboard Cite

“…Mass spectrometry approaches that provide a general scan of archaeological protein residues from cooking pottery, on the other hand, may not pinpoint original residues because exogenous proteins can overwhelm their signal (but see Cappellini et al 2010;Heaton et al 2009;Nielsen-Marsh 2005;Solazzo et al 2008). Put simply, when using mass spectrometry, a morass of exogenous protein from soil bacteria can overwhelm the signals of more archaeologically meaningful residues leaving them undetected.…”

Section: Immunoassay and Lc-msmentioning

confidence: 99%

“…Analysis of ancient bone proteins has witnessed substantial advancement during the last decade (see Buckley and Wadsworth 2014;Cappellini et al 2014;Welker et al 2015), some of which can be attributed to the peptide fingerprinting methodology, ZooMS, (Buckley et al 2009;van Doorn et al 2011) but which also relates to the general applicability of high throughput MS to bone (Cappellini et al 2012. In contrast, MS analysis of ancient protein residues from artifacts for the study of past subsistence has lagged (Barker et al 2012, Barnard 2007; but see Heaton et al 2009;Solazzo et al 2008). To address this deficiency, our paper focuses on the analysis of bone proteins using the extraction methodology of Barker et al (2012; Data, Methods & Taxonomies sive evaluation of our methodology as applied to multiple taxa to identify ancient proteins from several sites.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis

Barker

Dombrosky

Chaput

et al. 2015

EBL

View full text Add to dashboard Cite

Identification of protein residues from prehistoric cooking pottery using mass spectrometry is challenging because proteins are removed from original tissues, are degraded from cooking, may be poorly preserved due to diagenesis, and occur in a palimpsest of exogenous soil proteins. In contrast, bone proteins are abundant and well preserved. This research is part of a larger method-development project for innovation and improvement of liquid chromatography – mass spectrometry analysis of protein residues from cooking pottery; here we validate the potential of our extraction and characterization approach via application to ancient bone proteins. Because of its preservation potential for proteins and given that our approach is destructive, ancient bone identified via skeletal morphology represents an appropriate verification target. Proteins were identified from zooarchaeological turkey (Meleagris gallopavo Linnaeus Phasianidae), rabbit (Lagomorpha), and squirrel (Sciuridae) remains excavated from ancient pueblo archaeological sites in southwestern Colorado using a non-targeted LC-MS/MS approach. The data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD002440. Improvement of highly sensitive targeted LC-MS/MS approaches is an avenue for future method development related to the study of protein residues from artifacts such as stone tools and pottery.

show abstract

Towards the application of desorption electrospray ionisation mass spectrometry (DESI-MS) to the analysis of ancient proteins from artefacts

Cited by 46 publications

References 49 publications

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Archaeological Protein Residues: New Data for Conservation Science

Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis

Contact Info

Product

Resources

About