Archaeological Protein Residues: New Data for Conservation Science

Barker, Andrew

doi:10.14237/ebl.1.2010.58-65

Cited by 1 publication

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This approach had mainly been applied to artifact residues from tightly controlled cooking experiments (Barker et al 2012;Stevens et al 2010) or as identification of bone proteins from individual specimens (Barker 2011;Wolverton et al 2014). Our results indicate that our approach, which includes optimized extraction and solvent parameters from previous research (Barker et al 2012), leads to reliable identification of proteins when they are extracted from ancient bone.…”

Section: Discussionmentioning

confidence: 76%

“…Their utility for bone protein extraction was preliminarily validated through previous attempts using individual samples of archaeological bone (see Barker 2011;Wolverton et al 2014), but were here applied for the first time to a relatively large and diverse collection of specimens. Briefly, we pulverized a portion of each bone sample in a sterile mortar and pestle.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis

Barker

Dombrosky

Chaput

et al. 2015

EBL

Self Cite

View full text Add to dashboard Cite

Identification of protein residues from prehistoric cooking pottery using mass spectrometry is challenging because proteins are removed from original tissues, are degraded from cooking, may be poorly preserved due to diagenesis, and occur in a palimpsest of exogenous soil proteins. In contrast, bone proteins are abundant and well preserved. This research is part of a larger method-development project for innovation and improvement of liquid chromatography – mass spectrometry analysis of protein residues from cooking pottery; here we validate the potential of our extraction and characterization approach via application to ancient bone proteins. Because of its preservation potential for proteins and given that our approach is destructive, ancient bone identified via skeletal morphology represents an appropriate verification target. Proteins were identified from zooarchaeological turkey (Meleagris gallopavo Linnaeus Phasianidae), rabbit (Lagomorpha), and squirrel (Sciuridae) remains excavated from ancient pueblo archaeological sites in southwestern Colorado using a non-targeted LC-MS/MS approach. The data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD002440. Improvement of highly sensitive targeted LC-MS/MS approaches is an avenue for future method development related to the study of protein residues from artifacts such as stone tools and pottery.

show abstract