Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds

Baker, Simon C.; Southgate, Jennifer

doi:10.1016/j.biomaterials.2008.04.033

Cited by 54 publications

(46 citation statements)

References 36 publications

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“…The regeneration of SMCs was always assessed by anti-α-SMA antibody and anti-calponin antibody. Since α-SMA was involved in the contraction of SMCs, it was universally recognized as the symbol of the beginning of SMC differentiation [32, 33]. Since the expression of α-SMA increases after implantation, it indicated that the number of SMCs increased during the course of 6 months after implantation.…”

Section: Discussionmentioning

confidence: 99%

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

et al. 2018

J Vasc Res

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In the field of vascular graft research, poly-ε-caprolactone (PCL) is used owing to its good mechanical strength and biocompatibility. In this study, PCL scaffold was prepared by electrospinning and surface modification with heparin via hexamethylenediamine. Then the scaffolds were implanted into the infrarenal abdominal aorta of Wistar rats and contrast-enhanced micro-ultrasound was used to monitor the patency of grafts after implantation. These grafts were extracted from the rats at 1, 3, and 6 months for histological analysis, immunofluorescence staining, and scanning electron microscopy observation. Although some grafts experienced aneurysmal change, results showed that all implanted grafts were patent during the course of 6 months and these grafts demonstrated well-organized neotissue with endothelium formation, smooth muscle regeneration, and extracellular matrix formation. Such findings confirm feasibility to create heparin-conjugated scaffolds of next-generation vascular grafts.

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Section: Discussionmentioning

confidence: 99%

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

et al. 2018

J Vasc Res

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“…The sacrificial PEO fibers are interspersed inside the threedimensional matrix and dissolve easily in water and alcohol during sterilization procedure before cell seeding. The resulting interspaces between the residual fibers then enable cells to migrate through the matrix (Baker et al 2008). Though the control of pore size and interspaces in orientated nanofiber matrices is still challenging, the electrospinning technique holds great potential for TE and regenerative medicine and therefore pretends to be the most promising matrix for skeletal muscle TE at the moment.…”

Section: Matricesmentioning

confidence: 99%

“…Gelain, 2008) Baker et al used a 3D electrospun nanoscaffold of polystyrene to determine whether the phenotype of cells isolated from the stroma of human ureter specimens could be modulated by growing cells within such scaffold. (Baker, 2008) Non-aligned scaffolds made out of fibers with mean diameter of 200 nm were used in that study. Unfortunately the analysis of SM-MHC protein expression in cultures grown in 3D revealed that those cultures failed to undergo differentiation.…”

Section: Nano-and Microstructured Surfaces For Bladder Regenerationmentioning

confidence: 99%

“…3) that hinder cell infiltration (Baker & Mauck 2007, Telemeco et al 2005. Therefore, co-spinning of water-soluble sacrificial fibers such as poly-(ethylene-oxide) (PEO) has been shown to overcome this problem (Baker et al 2008). The sacrificial PEO fibers are interspersed inside the threedimensional matrix and dissolve easily in water and alcohol during sterilization procedure before cell seeding.…”

Section: Matricesmentioning

confidence: 99%

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Tissue Engineering for Tissue and Organ Regeneration

Eberli¹

2011

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“…Polystyrene (PS) is one of polymers used to produce cation exchange fibers [9]. PS nanofibers were successfully produced using the electrospinning method and it has been demonstrated that electrospun PS nanofibers have interesting applications in many areas [10][11][12]. The morphology of the PS nanofibers plays an important role in the physical/mechanical properties of the final products to be used in certain applications.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Cationic Exchange Polystyrene Nanofibers for Drug Delivery

Akkaramongkolporn¹,

Opanasopit

2014

Trop. J. Pharm Res

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Purpose: To prepare polystyrene nanofiber ion exchangers (PSNIE) with surface cation exchange functionality using a new method based on electrospinning and also to optimize crosslinking and sulfonation reactions to obtain PSNIE with maximum ion exchange capacity (IEC). Method: The nanofibers were prepared from 15% w/v polystyrene solution in dimethylacetamide (DMAc) containing 0.025 %w/v tetrabutylammonium bromide (TBAB) using electrospinning technique, followed by crosslinking with sulfuric acid/formaldehyde in a ratio ranging from 100/0 to 50/50 v/v and sulfonation in sulfuric acid. Degree of crosslinking was determined as the amount of fibers that remained in dichloromethane. The morphology and diameter of the fibers were evaluated by scanning electron microscopy (SEM) while IEC of PSNIE was performed by salt splitting titration. Results: PSNIE crosslinked with a sulfuric acid/formaldehyde ratio of 90/10 with 0.1 %w/v silver sulfate for 10 min at 70  C and sulfonated in 98 % sulfuric acid with 0.2 %w/v silver sulfate as the catalyst at 100  C for 30 min showed a maximum IEC of 3.21 meq/g-dry-PSNIE. Increase in sulfonation temperature caused the IEC of PSNIE to increase due to faster sulfonation. It was observed that the higher the temperature the faster the rate of sulfonation reaction. The diameter of the fibers after sulfonation was 404 ± 42 nm. Conclusion: These results indicate that PSNIE can be successfully prepared by electrospinning. Furthermore, cationic drug can be loaded onto the novel PSNIE for controlled release delivery.

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Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds

Cited by 54 publications

References 36 publications

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

Tissue Engineering for Tissue and Organ Regeneration

Fabrication of Cationic Exchange Polystyrene Nanofibers for Drug Delivery

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