Towards an affinity-free, centrifugal microfluidic system for rapid, automated forensic differential extraction

Woolf, M. Shane; Cunha, Larissa L.; Hadley, Kevyn C.; Moffett, Rachel P.; Landers, James P.

doi:10.1016/j.aca.2023.340826

Cited by 4 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional details (e.g., photos, schematics, and digital rendering) are available in previous publications. [25][26][27] On-disc, orthogonal ow, immunocapture Mouse, whole molecule, IgG (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, U.S.A.) was immobilized on a 4 mm (2.5 ml at 5.5 mg ml −1 ), circular nitrocellulose (NC) membrane cutout (0.2 mm pore size, Bio-Rad). Prior to lamination into the disc, each antibody-spotted NC cutout was dried for 60 min at 37 °C in a convection oven (Thermo Scientic™ PR305220G, Thermo Fisher Scientic Inc., Waltham, MA, U.S.A.).…”

Section: Mechatronic Spin Systemmentioning

confidence: 99%

Digital image analysis for biothreat detection via rapid centrifugal microfluidic orthogonal flow immunocapture

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Mechatronic Spin Systemmentioning

confidence: 99%

Digital image analysis for biothreat detection via rapid centrifugal microfluidic orthogonal flow immunocapture

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…An oligonucleotide strandattached magnetic ionic liquid was designed by Chen et al [34] for sequence-specific DNA extraction with a recovery efficiency of around 81%. There are also other microfluidic methods for DNA extraction, such as using cellulose membrane [35] and centrifugal force [36], the characteristics of which are listed in Table 2. In general, microfluidics could considerably improve the efficiency of DNA extrac-tion while reducing time and personnel for various types of samples and experimental demands.…”

Section: F I G U R Ementioning

confidence: 99%

Application of microfluidic technologies in forensic analysis

Guo,

Zhang,

Zhang

et al. 2023

Electrophoresis

View full text Add to dashboard Cite

The application of microfluidic technology in forensic medicine has steadily expanded over the last two decades due to the favorable features of low cost, rapidity, high throughput, user‐friendliness, contamination‐free, and minimum sample and reagent consumption. In this context, bibliometric methods were adopted to visualize the literature information contained in the Science Citation Index Expanded from 1989 to 2022, focusing on the co‐occurrence analysis of forensic and microfluidic topics. A deep interpretation of the literature was conducted based on co‐occurrence results, in which microfluidic technologies and their applications in forensic medicine, particularly forensic genetics, were elaborated. The purpose of this review is to provide an impartial evaluation of the utilization of microfluidic technology in forensic medicine. Additionally, the challenges and future trends of implementing microfluidic technology in forensic genetics are also addressed.

show abstract