Towards a catalogue of genes transcribed in multiple tissues of Mytilus galloprovincialis

Venier, Paola; Pallavicini, Alberto; Nardi, Barbara De; Lanfranchi, Gerolamo

doi:10.1016/s0378-1119(03)00708-x

Cited by 63 publications

(45 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on brain transcriptome against viral infections are not abundant in higher vertebrates (Gruslin et al, 2005;Paulus et al, 2006) Table 1. For the reverse library (genes up-regulated in normal tissues), there was mostly abundance of ribosomal and metabolic transcripts, revealing genes highly expressed for energy production and translation (Venier et al, 2003) (Table 2). The best matching was found for adenin nucleotide translocator s6 (BlastX, expectation value 1e-78).…”

Section: Resultsmentioning

confidence: 99%

Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus

Dios

Poisa-Beiro

Figueras

et al. 2007

Molecular Immunology

View full text Add to dashboard Cite

Despite of the impact that viruses have on aquatic organisms, relatively little is known on how fish fight against these infections. In this work, the brain gene expression pattern of sea bream (Sparus aurata) in response to nodavirus infection was investigated. We used the SSH (Supression Subtractive Hybridization) method to generate a subtracted cDNA library enriched with gene transcripts differentially expressed after one day post infection. Some of the ESTs from the infected tissues fell in gene categories related to stress and immune responses. For the reverse library (ESTs expressed in controls compared with infected tissues) the most abundant transcripts were of ribosomal and mitochondrial nature. Several ESTs potentially induced by virus exposure were selected for in vivo expression studies. We observed a clear difference in expression between infected and control samples for two candidate genes, ubiquitin conjugating enzyme 7 interacting protein, which seems to play an important role in apoptosis and the interferon induced protein with helicase C domain 1 (mda-5) that contributes to apoptosis and regulates the type I IFN production, a key molecule of the antiviral innate response in most organisms.

show abstract

Section: Resultsmentioning

confidence: 99%

Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus

Dios

Poisa-Beiro

Figueras

et al. 2007

Molecular Immunology

View full text Add to dashboard Cite

show abstract

“…Microarrays were designed using approximately 26,000 publicly available ESTs from M. californianus [ES7325872-ES738966; ES408175-ES387463 (Gracey et al, 2008)] and M. galloprovincialis [AJ623313-AJ626468; AJ516092-AJ516921 (Venier et al, 2003)]. Both sets of ESTs were sequenced from libraries constructed using Suppression Subtractive Hybridization (SSH) (Diatchenko et al, 1996) to enrich for transcripts expressed in response to various stressors.…”

Section: Microarray Developmentmentioning

confidence: 99%

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

Lockwood

Sanders

Somero

2010

Journal of Experimental Biology

223

279

View full text Add to dashboard Cite

SUMMARYInvasive species are increasingly prevalent in marine ecosystems worldwide. Although many studies have examined the ecological effects of invasives, little is known about the physiological mechanisms that might contribute to invasive success. The mussel Mytilus galloprovincialis, a native of the Mediterranean Sea, is a successful invader on the central and southern coasts of California, where it has largely displaced the native congener, Mytilus trossulus. It has been previously shown that thermal responses of several physiological traits may underlie the capacity of M. galloprovincialis to out-compete M. trossulus in warm habitats. To elucidate possible differences in stress-induced gene expression between these congeners, we developed an oligonucleotide microarray with 8874 probes representing 4488 different genes that recognized mRNAs of both species. In acute heat-stress experiments, 1531 of these genes showed temperature-dependent changes in expression that were highly similar in the two congeners. By contrast, 96 genes showed species-specific responses to heat stress, functionally characterized by their involvement in oxidative stress, proteolysis, energy metabolism, ion transport, cell signaling and cytoskeletal reorganization. The gene that showed the biggest difference between the species was the gene for the molecular chaperone small heat shock protein 24, which was highly induced in M. galloprovincialis and showed only a small change in M. trossulus. These different responses to acute heat stress may help to explain -and predict -the invasive success of M. galloprovincialis in a warming world. Supplementary material available online at

show abstract

“…The genus Mytilus is common among marine mollusks; members of this genus occur along a wide latitudinal range in both hemispheres and play significant roles in the ecology of benthic and pelagic communities (Venier et al 2003;Aarab et al 2011). Members of the genus have become model study organisms because of their economic value in shellfish farming (Gueguen et al 2011) and their role as bioindicators (Moreira and Guilhermino 2005;Kayhan et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expressions of oxidative stress-related genes and antioxidant enzyme activities in Mytilus galloprovincialis (Bivalvia, Mollusca) exposed to hypoxia

et al. 2013

View full text Add to dashboard Cite

Background: In this study, we investigated transcription and enzyme level responses of mussels Mytilus galloprovincialis exposed to hypoxic conditions. Genes for catalase (CAT), cytochrome P450, glutathione Stransferase (GST), metallothionein, superoxide dismutase (SOD), cytochrome c oxidase subunit 1 (COX-1), and NADH dehydrogenase subunit 2 were selected for study. Transcriptional changes were investigated in mussels exposed to hypoxia for 24 and 48 h and were compared to changes in control mussels maintained at normal oxygen levels. Activities of CAT, GST, and SOD enzymes, and lipid peroxidation (LPO) were also investigated in mussels following exposure to hypoxia for 24, 48, and 72 h. Results: Relative to the control group, the CAT activity decreased in all hypoxia treatments, while the activity of GST significantly increased in mussels exposed to hypoxia for 24 and 48 h, but decreased in those exposed for 72 h. The LPO levels were significantly higher in mussels in the 24-and 48-h hypoxia treatments than those in the control mussels, but there was no significant change in the SOD activities among all hypoxia treatments. Messenger RNA levels for the CAT, cytochrome P450, GST, metallothionein, and SOD genes were not significantly affected by hypoxic conditions for 48 h, but the expressions of the COX-1 and NADH dehydrogenase subunit 2 genes were significantly repressed in mussels in both the 24-and 48-h exposure treatments.

show abstract

Towards a catalogue of genes transcribed in multiple tissues of Mytilus galloprovincialis

Cited by 63 publications

References 40 publications

Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus

Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

Expressions of oxidative stress-related genes and antioxidant enzyme activities in Mytilus galloprovincialis (Bivalvia, Mollusca) exposed to hypoxia

Contact Info

Product

Resources

About