Toward plasmonic monitoring of surface effects on bacterial quorum-sensing

Hill, Eric H.; Liz‐Marzán, Luis M.

doi:10.1016/j.cocis.2017.04.003

Cited by 12 publications

(7 citation statements)

References 72 publications

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“…Consequently, they allow sensitive biomolecule detection with substantially reduced material costs and preparative effort, as demonstrated with the bacterial pathogen pyocyanin both in water and Luria–Bertrani medium. The capability of these substrates for detecting bacterial quorum-sensing molecules in bacterial growth medium, even without an additional filtration layer, should facilitate in situ studies of bacterial growth/quorum sensing via SERS and could be particularly useful for studying the effects of surface topography on bacterial attachment and biofilm formation . Finally, we propose that the particle assembly mechanism discussed herein is of a universal nature and could have important implications for the field of template-assisted colloidal assembly.…”

Section: Discussionmentioning

confidence: 91%

“…The capability of these substrates for detecting bacterial quorum-sensing molecules in bacterial growth medium, even without an additional filtration layer, should facilitate in situ studies of bacterial growth/quorum sensing via SERS and could be particularly useful for studying the effects of surface topography on bacterial attachment and biofilm formation. 53 Finally, we propose that the particle assembly mechanism discussed herein is of a universal nature and could have important implications for the field of template-assisted colloidal assembly. We therefore expect this proof of concept to trigger deeper investigations utilizing nanoparticles of varied materials composition and surface functionalization, as well as the exploration of a plethora of pattern designs and target substrates.…”

Section: ■ Conclusionmentioning

confidence: 87%

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Solvent-Assisted Self-Assembly of Gold Nanorods into Hierarchically Organized Plasmonic Mesostructures

Hanske

Hill

Vila‐Liarte

et al. 2019

ACS Appl. Mater. Interfaces

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Plasmonic supercrystals and periodically structured arrays comprise a class of materials with unique optical properties that result from the interplay of plasmon resonances, as well as near- and far-field coupling. Controlled synthesis of such hierarchical structures remains a fundamental challenge, as it demands strict control over the assembly morphology, array size, lateral spacing, and macroscale homogeneity. Current fabrication approaches involve complicated multistep procedures lacking scalability and reproducibility, which has hindered the practical application of plasmonic supercrystal arrays. Herein, these challenges are addressed by adding an organic solvent to achieve kinetic control over the template-assisted colloidal assembly of nanoparticles from aqueous dispersion. This method yields highly regular periodic arrays, with feature sizes ranging from less than 200 nm up to tens of microns. A combined experimental/computational approach reveals that the underlying mechanism is a combination of the removal of interfacial surfactant micelles from the particle interface and altered capillary flows. Assessing the efficacy of such square arrays for surface-enhanced Raman scattering spectroscopy, we find that a decrease of the lattice periodicity from 750 nm down to 400 nm boosts the signal by more than an order of magnitude, thereby enabling sensitive detection of analytes, such as the bacterial quorum sensing molecule pyocyanin, even in complex biological media.

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Section: Discussionmentioning

confidence: 91%

Section: ■ Conclusionmentioning

confidence: 87%

Solvent-Assisted Self-Assembly of Gold Nanorods into Hierarchically Organized Plasmonic Mesostructures

Hanske

Hill

Vila‐Liarte

et al. 2019

ACS Appl. Mater. Interfaces

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100

105

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“…Many important pathogens regulate virulence via QS, which is a bacterial cell-to-cell communication system utilizing small signal molecules (Turan et al, 2017; Defoirdt, 2018; Prajapat and Saini, 2018). QS plays an important role in biofilm formation, pathogenicity, and virulence of pathogenic bacteria (Adonizio et al, 2006; Han et al, 2011; Husain et al, 2013; Hill and Liz-Marzán, 2017; Padder et al, 2018). The virulence and pathogenicity of pathogenic bacteria can be inhibited by inhibiting their QS.…”

Section: Introductionmentioning

confidence: 99%

Diallyl Disulfide From Garlic Oil Inhibits Pseudomonas aeruginosa Quorum Sensing Systems and Corresponding Virulence Factors

Xie

et al. 2019

Front. Microbiol.

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Previously, we determined that diallyl disulfide (DADS) from garlic oil can inhibit Pseudomonas aeruginosa PAO1 pathogenic factors by inactivating the transcription of key genes from three quorum sensing (QS) systems (las, rhl, and pqs) based on the effects of DADS on growth, virulence factor production (elastase, pyocyanin, biofilm, and swarming motility), and RNA transcription (real-time q-PCR). To further investigate the mechanisms underlying the inhibition of the three P. aeruginosa QS systems by DADS, high-throughput RNA and proteome sequencing techniques were used to study differences in the transcriptional and proteome expression of P. aeruginosa PAO1 following treatment with DADS. The RNA-seq and proteomic data are available via NCBI Gene Expression Omnibus database with accession number GSE118801 and ProteomeXchange with identifier PXD011144, respectively. The experimental results indicated that all key genes of the three QS systems (las, rhl, and pqs) of P. aeruginosa PAO1 as well as the virulence factors (including exoprotease LasA, elastase LasB, lectin LecA and LecB, pyocyanin biosynthesis, and biofilm formation) regulated by these three QS systems were inhibited. This is consistent with our previous studies on the physiology, biochemistry, and RNA expression of P. aeruginosa treated with DADS. Additionally, our results also indicated that bacterial motility, chemotaxis, and two-component systems were inhibited by DADS treatment. All these changes abolish the sensitivity of P. aeruginosa PAO1 to environmental stimuli and cause the cells to be in a state of passivation. Further research is needed to determine how QS systems regulate these functions. Our findings could potentially contribute to the treatment and control of P. aeruginosa infection, virulence, and pathogenicity.

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“…2017) studied the mechanism of 3-phenyllactic acid (PLA) action and a QS inhibitory compounds. The results showed that PLA inhibited the virulence factors such as pyocyanin, protease, and rhamnolipids, which were correlated with the biofilm of P. aeruginosa PAO1.Biofilm formation facilitated by a cell-cell communication using QS enabled group behavior and metabolic regulation(Hill and Liz-Marzán, 2017). Surface topography and chemistry were important to evaluate bacterial adhesion and biofilm, but there was a limit to howQ S c a n b e m o n i t o r e d i n s i t u .…”

mentioning

confidence: 97%