Toward greener analytical techniques for the absolute quantification of peptides in pharmaceutical and biological samples

Eeckhaut, Ann Van; Mangelings, Debby

doi:10.1016/j.jpba.2015.03.023

Cited by 15 publications

(6 citation statements)

References 80 publications

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“…Profiteering of separation and documentation methods such as chromatographic isolation and electrophoresis have been used for a prolonged period. These techniques have principally been targeted to carefully comprehend either the physical or chemical stabilities, or the bioanalysis of peptides at the highest degree of their sensitivity, by identifying the amino- and carboxy-terminal residues and the amino acid sequences [ 185 , 186 , 187 ]. For example, structural identification and detection focusing on physical stability of peptides has been mainly achieved through the SEC, by examining the elution volume, time of migration and peptide aggregation at higher rates [ 188 ].…”

Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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Food-based components represent major sources of functional bioactive compounds. Milk is a rich source of multiple bioactive peptides that not only help to fulfill consumers ‘nutritional requirements but also play a significant role in preventing several health disorders. Understanding the chemical composition of milk and its products is critical for producing consistent and high-quality dairy products and functional dairy ingredients. Over the last two decades, peptides have gained significant attention by scientific evidence for its beneficial health impacts besides their established nutrient value. Increasing awareness of essential milk proteins has facilitated the development of novel milk protein products that are progressively required for nutritional benefits. The need to better understand the beneficial effects of milk-protein derived peptides has, therefore, led to the development of analytical approaches for the isolation, separation and identification of bioactive peptides in complex dairy products. Continuous emphasis is on the biological function and nutritional characteristics of milk constituents using several powerful techniques, namely omics, model cell lines, gut microbiome analysis and imaging techniques. This review briefly describes the state-of-the-art approach of peptidomics and lipidomics profiling approaches for the identification and detection of milk-derived bioactive peptides while taking into account recent progress in their analysis and emphasizing the difficulty of analysis of these functional and endogenous peptides.

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Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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“…In majority of the above applications of (bio)synthetic or from natural sources isolated peptides, CE and CEC can be used as sensitive control methods for the assessment of peptide purity. As shown in the recent reviews , CE and CEC methods provide fast and accurate qualitative and quantitative information about the peptide drugs. Further, some recent CE and CEC applications to peptide analyses will be given, in addition to those presented already in the previous sections.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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“…Exploitation of separation and documentation methods such as electrophoresis and chromatographic isolation has been practiced for a long time. These techniques have principally been targeted to carefully comprehend either the physical or chemical stabilities, or the bioanalysis of peptides at the highest degree of their sensitivity, by identifying the amino‐ and carboxy‐terminal residues and the amino acid sequences (Khalaf, Baur, & Pfister, ; Underberg, Hoitink, Reubsaet, & Waterval, ; Van Eeckhaut & Mangelings, ). For instance, structure‐based detection targeting physical stability of the peptides have mainly been achieved through size‐exclusion chromatography (SEC), by investigating elution volume, migration time, and peptide aggregation at high concentrations (Davio, Kienle, & Collins, ).…”

Section: Structure‐informed Detection and Quantitation Of Peptidesmentioning

confidence: 99%

Structure-informed detection and quantification of peptides in food and biological fluids

et al. 2017

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Peptides with biological properties, that is, bioactive peptides, are a class of biomolecules whose health-promoting properties are increasingly being exploited in food and health products.However, research on targeted techniques for the detection and quantification of these peptides is still in its infancy. Such information is needed in order to enhance the biological and chemometric characterization of peptides and their subsequent application in the functional food and pharmaceutical industries. In this review, the role of classic techniques such as electrophoretic, chromatographic, and peptide mass spectrometry in the structure-informed detection and quantitation of bioactive peptides are discussed. Prospects for the use of aptamers in the characterization of bioactive peptides are also discussed. Practical applicationsAlthough bioactive peptides have huge potential applications in the functional foods and health area, there are limited techniques in enhancing throughput detection, quantification, and characterization of these peptides. This review discusses state-of-the-art techniques relevant in complementing bioactive detection and profiling irrespective of the small number of amino acid units. Insights into challenges, possible remedies and prevailing areas requiring thorough research in the extant literature for food chemists and biotechnologists are also presented.

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Toward greener analytical techniques for the absolute quantification of peptides in pharmaceutical and biological samples

Cited by 15 publications

References 80 publications

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Structure-informed detection and quantification of peptides in food and biological fluids

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