Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinically significant mass

Sakai, Yasuyuki; Huang, Hongyun; Hanada, Sanshiro; Niino, Toshiki

doi:10.1016/j.bej.2009.10.010

Cited by 27 publications

(9 citation statements)

References 122 publications

(147 reference statements)

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“…Biocompatibility of many common quantum dots is questionable, 20,21 though more compatible forms are under development. 22 Nanorubies are an attractive though immature platform with limited commercial availability. Furthermore, the methodology to functionalize these particles is not well established compared to nanodiamonds.…”

Section: Discussionmentioning

confidence: 99%

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Gerstenhaber¹,

Barone²,

Marcinkiewicz³

et al. 2017

IJN

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The aim of this feasibility study was to test the ability of fluorescent nanodiamond particles (F-NDP) covalently conjugated with bitistatin (F-NDP-Bit) to detect vascular blood clots in vivo using extracorporeal near-infrared (NIR) imaging. Specifically, we compared NIR fluorescence properties of F-NDP with N-V (F-NDP NV ) and N-V-N color centers and sizes (100–10,000 nm). Optimal NIR fluorescence and tissue penetration across biological tissues (rat skin, porcine axillary veins, and skin) was obtained for F-NDP NV with a mean diameter of 700 nm. Intravital imaging (using in vivo imaging system [IVIS]) in vitro revealed that F-NDP NV -loaded glass capillaries could be detected across 6 mm of rat red-muscle barrier and 12 mm of porcine skin, which equals the average vertical distance of a human carotid artery bifurcation from the surface of the adjacent skin (14 mm). In vivo, feasibility was demonstrated in a rat model of occlusive blood clots generated using FeCl 3 in the carotid artery bifurcation. Following systemic infusions of F-NDP NV -Bit (3 or 15 mg/kg) via the external carotid artery or femoral vein (N=3), presence of the particles in the thrombi was confirmed both in situ via IVIS, and ex vivo via confocal imaging. The presence of F-NDP NV in the vascular clots was further confirmed by direct counting of fluorescent particles extracted from clots following tissue solubilization. Our data suggest that F-NDP NV -Bit associate with vascular blood clots, presumably by binding of F-NDP NV -Bit to activated platelets within the blood clot. We posit that F-NDP NV -Bit could serve as a noninvasive platform for identification of vascular thrombi using NIR energy monitored by an extracorporeal device.

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Section: Discussionmentioning

confidence: 99%

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Gerstenhaber¹,

Barone²,

Marcinkiewicz³

et al. 2017

IJN

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“…Owing to the importance of vasculatures for normal physiological liver functions, various strategies have been employed to guarantee the supply of oxygen and nutrients to hepatic cells. 115 , 116 One strategy is to implant hepatocyte aggregates near the existing blood vessels in vivo to promote angiogenesis. 117 As an example, Yap et al 9 implanted murine liver progenitor cells embedded in growth factor-reduced Matrigel into the vascularized chamber fitted around the superficial epigastric vascular pedicle at the groin of SCID mice.…”

Section: Bioengineering Of Functional Hepatic Constructsmentioning

confidence: 99%

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

et al. 2021

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Liver failure is a lethal condition with hepatocellular dysfunction, and liver transplantation is presently the only effective treatment. However, due to the limited availability of donors and the potential immune rejection, novel therapeutic strategies are actively sought to restore the normal hepatic architectures and functions, especially for livers with inherited metabolic dysfunctions or chronic diseases. Although the conventional cell therapy has shown promising results, the direct infusion of hepatocytes is hampered by limited hepatocyte sources, poor cell viability, and engraftment. Hence, this review mainly highlights the role of stem cells and progenitors as the alternative cell source and summarizes the potential approaches based on tissue engineering to improve the delivery efficiency of cells. Particularly, the underlying mechanisms for cell therapy using stem cells and progenitors are discussed in two main aspects: paracrine effect and cell differentiation. Moreover, tissue-engineering approaches using cell aggregates and decellularized liver scaffolds for bioengineering of functional hepatic constructs are discussed and compared in terms of the potential to replicate liver physiological structures. In the end, a potentially effective strategy combining the premium advantages of stem cell aggregates and decellularized liver scaffolds is proposed as the future direction of liver tissue engineering and regeneration.

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“…Since one of the main challenges in tissue engineering is to achieve sufficient O2 supply for regeneration of a compelling amount of tissue, [174] the authors also evaluated the potential of the as-prepared Hb-BSA microparticles for tissue engineering applications. [175] Employing HBOCs for tissue engineering purposes is of special relevance for liver cells, since they have a high demand for O2, [176][177][178][179] and it has been previously demonstrated that supplementing the circulating cell culture with HBOCs enhances the hepatocytes metabolism.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Recent and prominent examples of nano- and microarchitectures as hemoglobin-based oxygen carriers

Jansman

Hosta‐Rigau

2018

Advances in Colloid and Interface Science

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Toward engineering of vascularized three-dimensional liver tissue equivalents possessing a clinically significant mass

Cited by 27 publications

References 122 publications

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

Recent and prominent examples of nano- and microarchitectures as hemoglobin-based oxygen carriers

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