Toward Contactless Biology: Acoustophoretic DNA Transfection

Vasileiou, Thomas; Foresti, Daniele; Bayram, Adem; Poulikakos, Dimos; Ferrari, Aldo

doi:10.1038/srep20023

Cited by 64 publications

(40 citation statements)

References 40 publications

(50 reference statements)

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“…For instance, the frequency of SAWs vibrating at 100 MHz corresponds to a wavelength of less than 100 µm, making them desirable for higher resolution patterning. It has been shown that higher frequency acoustic waves can be used to precisely pattern cells and nanoparticles 287,293–297. Wu demonstrated the use of acoustic tweezers to trap latex microparticles and clusters of frog eggs in a potential well (regions of minimum potential energy) generated by two collimated, focused ultrasonic beams 298.…”

Section: Acoustic Patterningmentioning

confidence: 99%

Nanomaterial Patterning in 3D Printing

et al. 2020

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The synergistic integration of nanomaterials with 3D printing technologies can enable the creation of architecture and devices with an unprecedented level of functional integration. In particular, a multiscale 3D printing approach can seamlessly interweave nanomaterials with diverse classes of materials to impart, program, or modulate a wide range of functional properties in an otherwise passive 3D printed object. However, achieving such multiscale integration is challenging as it requires the ability to pattern, organize, or assemble nanomaterials in a 3D printing process. This review highlights the latest advances in the integration of nanomaterials with 3D printing, achieved by leveraging mechanical, electrical, magnetic, optical, or thermal phenomena. Ultimately, it is envisioned that such approaches can enable the creation of multifunctional constructs and devices that cannot be fabricated with conventional manufacturing approaches.

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Section: Acoustic Patterningmentioning

confidence: 99%

Nanomaterial Patterning in 3D Printing

et al. 2020

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“…This probe, dubbed TubeCamp, comprises the calcium-sensitive derivative of GFP, GCamp3 11 , fused with the microtubule-binding domain of ensconsin (EMTB) 12 . GCamp fluorescence emission increases upon calcium binding 11 while fusions of EMTB with fluorescent proteins have been used in mammalian 13 , amphibian 14, 15 and invertebrate 14 cells to label microtubules. To determine whether TubeCamp reports on microtubule-proximal elevated [Ca 2+ ] i , TubeCamp and R-Geco, a calcium-sensitive reporter protein 16 were expressed in Xenopus oocytes which were then wounded to elicit a local [Ca 2+ ] i increase and microtubule reorganization 17 .…”

Section: Figurementioning

confidence: 99%

Calcium nanodomains in spindles

Mo¹,

Swider²,

Tao³

et al. 2018

Preprint

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37The role of calcium signaling in specific events of animal cell meiosis or mitosis (M-38 phase) is a subject of enduring controversy. Early efforts suggested that increases in 39 intracellular free calcium ([Ca 2+ ] i ) promote spindle disassembly 1, 2 while subsequent 40 work suggested that global [Ca 2+ ] i increases trigger nuclear envelope breakdown, spindle 41 assembly, the metaphase-anaphase transition, and cytokinesis 3-6 . However, further 42 studies led to the conclusion that elevation of [Ca 2+ ] i either has no role in these events, 43 plays a permissive role in these events, or functions as an auxiliary signaling pathway 44 that supplements other mechanisms 7 . One potential explanation of the controversy is 45 that specific M-phase events might depend on highly localized increases in [Ca 2+ ] i , 46 variously referred to as microdomains 8 or nanodomains 9 , as proposed recently 10 . Such 47 domains are hypothesized to arise from rapid shuttling of calcium between closely 48 positioned sources and sinks, rendering them potentially difficult to detect with 49 traditional dyes and largely insensitive to slow chelators such as EGTA 9 . Here a novel 50 microtubule-binding calcium sensor-TubeCamp--was used to test the hypothesis 10 that 51 spindles are associated with calcium nanodomains. TubeCamp imaging revealed that 52 spindles in Xenopus eggs, Xenopus embryos, and HeLa cells were all associated with 53 calcium nanodomains at the spindle poles. Calcium nanodomains also formed in spindles 54 assembled in cell extracts and at the center of monopolar spindles, suggesting that they 55 are a basic feature of spindle self-assembly. Disruption of calcium nanodomains via 56 perturbation of inositol-1,4,5-trisphosphate signaling or rapid chelation of [Ca 2+ ]i resulted 57 in spindle disassembly in vivo and vitro. The results demonstrate the existence of 58 spindle-associated calcium nanodomains and indicate that such domains are an essential 59 and common feature of spindles in vertebrates. 60 61 To overcome the limitations of soluble calcium reporters, we developed a genetically 62 encoded probe designed to detect microtubule-proximal increases in [Ca 2+ ] i . This probe, 63 dubbed TubeCamp, comprises the calcium-sensitive derivative of GFP, GCamp3 11 , 64 fused with the microtubule-binding domain of ensconsin (EMTB) 12 . GCamp 65 fluorescence emission increases upon calcium binding 11 while fusions of EMTB with 66 fluorescent proteins have been used in mammalian 13 , amphibian 14, 15 and invertebrate 14 67 cells to label microtubules. To determine whether TubeCamp reports on microtubule-68 proximal elevated [Ca 2+ ] i , TubeCamp and R-Geco, a calcium-sensitive reporter protein 16 69were expressed in Xenopus oocytes which were then wounded to elicit a local [Ca 2+ ] i 70 increase and microtubule reorganization 17 . Before wounding, the oocyte cortex 71

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“…The acoustic levitation method (ALM) of sample manipulation [1][2][3][4][5][6] is of great importance and a promising candidate for potential mid-air lab-on-a-drop applications, including the solidification of materials 7 , chemical analysis 8,9 , X-ray crystallography 10 , DNA transfection 11 , blood analysis 12 , and microreactors 13 . ALM has been studied for the contactless handling of matter in air.…”

mentioning

confidence: 99%

Acoustic Manipulation of Droplets under Reduced Gravity

Hasegawa

Watanabe

Abe

2019

Sci Rep

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contactless manipulation of matter is essential for studying physical phenomena. Acoustic manipulation of liquid samples using ultrasonic phased arrays provides a novel and attractive solution for mid-air manipulation, such as levitation, transportation, coalescence, mixing, separation, evaporation, and extraction, with a simple and single sequence. Despite the importance of gravity in droplet dynamics, its effect on a levitated droplet with an ultrasonic phased array remains unclear. to disseminate acoustic manipulation, better understanding of the fundamental physics of a droplet manipulated by ultrasonic phased arrays is required. Here, we show contactless levitation, transportation, and coalescence of multiple droplets under both ground and reduced gravity. Under ground gravity, the possible levitation size of the sample is limited to below the half wavelength of sound. Under reduced gravity, however, droplets that are larger than the limit can be successfully levitated, transported, and coalesced. furthermore, the threshold of sound pressure for droplet levitation and manipulation could be minimised with the suppression of nonlinear acoustic phenomena under reduced gravity. these insights promote the development of contactless manipulation techniques of droplets for future space experiment and inhabitancy.

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Toward Contactless Biology: Acoustophoretic DNA Transfection

Cited by 64 publications

References 40 publications

Nanomaterial Patterning in 3D Printing

Nanomaterial Patterning in 3D Printing

Calcium nanodomains in spindles

Acoustic Manipulation of Droplets under Reduced Gravity

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