Toward Bioelectronics:  Specific DNA Recognition Based on an Oligonucleotide-Functionalized Polypyrrole

Korri-Youssoufi, Hafsa; Garnier, F.; Srivastava, Pratima; Godillot, and P.; Yassar, A.

doi:10.1021/ja964261d

Cited by 339 publications

(209 citation statements)

References 25 publications

(22 reference statements)

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“…Moreover, the E-DNA sensor achieves the significant advantages of reagentless, reusable operation without compromising sensitivity or selectivity; the Ϸ10 pM sensitivity and greater than million-fold selectivity of the E-DNA sensor is competitive with the very best current electrochemical DNA sensors. For example, E-DNA's 10-pM detection limit (Ϸ5 fmol in 0.5 ml) matches both sandwich approaches (J. F. Kayyem, personal communication) and a recently proposed enzymeamplified electrochemical detection method (32,39), and is only surpassed by the 0.5 pM detection limit achieved by the reagentintensive, nonreusable catalytic amplification approach of Mirkin and coworkers (8).…”

Section: Discussionmentioning

confidence: 52%

“…2 Inset). Whereas similar logarithmic signal-versus-concentration relationships have been reported for other solid-state DNA sensors (32,39), the mechanism underlying the relationship has not been determined.…”

Section: Resultsmentioning

confidence: 78%

“…Despite these advances, there has been relatively little progress toward the important goal of creating electrochemical DNA detection methods that are simultaneously sensitive, selective, and reagentless (32); all of the above-described electrochemical hybridization detectors require posthybridization treatment with either hybridization indicators or other exogenous signaling molecules. Here, we report the development of a sensitive, reagentless, reusable electrochemical DNA sensor that combines the significant advantages of electrochemical detection with the versatility of reagentless, reusable, surface-attached molecular beacons.…”

mentioning

confidence: 99%

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Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Fan

Plaxco²,

Heeger³

2003

Proc. Natl. Acad. Sci. U.S.A.

1,004

896

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We report a strategy for the reagentless transduction of DNA hybridization into a readily detectable electrochemical signal by means of a conformational change analogous to the optical molecular beacon approach. The strategy involves an electroactive, ferrocene-tagged DNA stem-loop structure that self-assembles onto a gold electrode by means of facile gold-thiol chemistry. Hybridization induces a large conformational change in this surface-confined DNA structure, which in turn significantly alters the electron-transfer tunneling distance between the electrode and the redoxable label. The resulting change in electron transfer efficiency is readily measured by cyclic voltammetry at target DNA concentrations as low as 10 pM. In contrast to existing optical approaches, an electrochemical DNA (E-DNA) sensor built on this strategy can detect femtomoles of target DNA without employing cumbersome and expensive optics, light sources, or photodetectors. In contrast to previously reported electrochemical approaches, the E-DNA sensor achieves this impressive sensitivity without the use of exogenous reagents and without sacrificing selectivity or reusability. The E-DNA sensor thus offers the promise of convenient, reusable detection of picomolar DNA.

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Section: Discussionmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 78%

mentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Fan

Plaxco²,

Heeger³

2003

Proc. Natl. Acad. Sci. U.S.A.

1,004

896

View full text Add to dashboard Cite

show abstract

“…or metal-ion coordination as basic interactions between the components. Due to these two properties, DNA molecules seem particularly suitable to be used as components for the construction of nanometer scale devices [23][24][25][26]. The idea of using DNA in molecular devices is its natural function of storing and coding the genetic information.…”

Section: Dna Biomolecular Electronicsmentioning

confidence: 99%

Biomolecules and Pure Carbon Aggregates: An Application Towards “Green Electronics”

Srivastava¹

2018

Green Electronics

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Abstract"Green electronics" is a novel scientific term which aims to identify the compounds of natural origin (economically safe and biodegradable) and establish economically efficient route for production of synthetic materials. The purpose of green electronics is to create path for the production of human and environmental friendly electronics and the integration of electronics with living tissue in particular. These researches may help to fulfill not only the organic electronics to deliver low cost energy efficient materials and devices, but also achieve unimaginable functionalities for electronics. In this chapter we have considered the molecular electronic devices biomolecules: deoxyribonucleic acid (DNA) and pure carbon aggregates: (carbon nanotubes (CNTs)/graphene), their properties and applications.

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“…13,14 The scope of polypyrroles can be considerably extended when functions, such as reactive groups, biological recognition functions, ligands for metal complexation, or catalytic units are introduced. 15,16 As attachment points positions 1 and 3 of the pyrrole ring are preferred, because oxidative polymerizations of pyrroles to PPy run preferably via positions 2 and 5. In most cases, the functions are not directly attached to the pyrrole ring but are connected via linkers.…”

Section: Introductionmentioning

confidence: 99%

Linking applicatory functions to the 3-position of pyrrole by click chemistry

Karsten¹,

Nan²,

Liebscher³

2012

Arkivoc

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A straight forward and reliable method was developed to tether recognition functions to a side chain in position 3 of pyrroles via triazole linkage. The products are precursors for functionalized polypyrroles, e. g. for coating magnetic nanoparticles or selective electrodes. A pyrrole with an azido function located at the terminus of a side chain in position 3 was submitted to coppercatalyzed Meldal-Sharpless click reaction with alkynes bearing biotin, nitrilotriacetic acid or a RGD-containing cyclopentapeptide. The latter presents a very versatile building block for the introduction of the RGD-moiety in a variety of potential substrates.

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Toward Bioelectronics: Specific DNA Recognition Based on an Oligonucleotide-Functionalized Polypyrrole

Cited by 339 publications

References 25 publications

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Biomolecules and Pure Carbon Aggregates: An Application Towards “Green Electronics”

Linking applicatory functions to the 3-position of pyrrole by click chemistry

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