Total yield of reactive species originating from an atmospheric pressure plasma jet in real time

Abstract

It is now well established that plasma-induced reactive species are key agents involved in many biochemical reactions. This work reports on the formation of plasma reactive species in an acidified ferrous sulfate (Fricke) solution interacting with an atmospheric pressure plasma jet (APPJ). A yield of ferric (Fe3+) ions measured using in situ absorption spectroscopy was attributed to the formation of plasma reactive species provided and/or originated in the solution. The results indicated that the number of reactive species formed was proportional to plasma frequency and voltage. However, the Fe3+ yield per pulse decreased with increased frequency. To obtain a better understanding of the processes and species involved in the chemical reactions due to plasma exposure, Fe3+ yields were calculated and compared to the experimental data. At higher frequencies, there was insufficient time to complete all the reactions before the next pulse reached the solution; at lower frequencies, the Fe3+ yield was higher because of the relatively longer time available for reactions to occur. In addition, the comparison between DNA damage levels and Fe3+ yields was investigated under different experimental conditions in order to verify the usefulness of both the Fricke solution and the DNA molecule as a probe to characterize APPJs.

Revealing low-temperature plasma efficacy through a dose-rate assessment by DNA damage detection combined with machine learning models

“…Generally, in radiation research, the dose-rate effect is associated with the yield of specific reactive species, which induce chemical alterations in the biomolecular target 13 . Using a traditional chemical dosimeter in our previous study, we observed an increase in the total amount of reactive species as a function of frequency and voltage for the same LTP source 15 . Thus, the increase in the estimated dose rate with a frequency and voltage in this study can be related to the increase in the number and/or type of plasma species responsible for DNA damage.…”

Effect of atmospheric cold plasma (ACP) on chlorine-adapted Salmonella enterica on spring onion

“…2015; Adhikari et al . 2019) while measuring intermediate and short‐lived reactive species is neglected most of the time (Adhikari et al . 2019).…”

“…2019) while measuring intermediate and short‐lived reactive species is neglected most of the time (Adhikari et al . 2019). Thus, other mechanisms may be involved in the antimicrobial activity of plasma against natural flora compared to inoculated pathogenic microorganisms such as S. enterica .…”

“…These species quickly convert to longer-lived species such as hydrogen peroxide, nitrites, nitrates and other uncertain intermediate species (Patange et al 2019a). Most studies have focused on the inactivation of natural flora at a fixed high voltage since long-lived reactive species increase in proportion with voltage and frequency (Ziuzina et al 2015;Adhikari et al 2019) while measuring intermediate and short-lived reactive species is neglected most of the time (Adhikari et al 2019). Thus, other mechanisms may be involved in the antimicrobial activity of plasma against natural flora compared to inoculated pathogenic microorganisms such as S. enterica.…”

Acoustic characterization of atmospheric-pressure dielectric barrier discharge plasma jets

“…where p is the ambient pressure, δp is the pressure change, 63 T is the steady-state temperature, δT is the tempera-64 ture change and γ is the heat capacity ratio for helium 65 (1.66 at 293 K). Using ambient pressure of one atmosphere 66 (101 kPa) and a steady state temperature of 433 K, a tem-67 perature change of 0.8 K corresponds to a pressure change 68 of ±469 Pa. An SPL can be calculated from It is relevant to note that in our previous studies on the 33 biological effects of APPJ on biomolecular targets, which 34 were performed using the same plasma source and the 35 same electrical conditions, we observed damage to DNA 36 [32,33]. Thus, it indicates that the source that can be oper-37 ated at the safety sound level is capable of producing the 38 plasma components that can induce alternation to the cellular components.…”