Total polysaccharides of adlay bran (<i>Coix lachryma-jobi</i>L.) improve TNF-α induced epithelial barrier dysfunction in Caco-2 cells<i>via</i>inhibition of the inflammatory response

Li, Yanlong; Tian, Xudong; Sheng-cai, LI; Chang, Lijun; Sun, Ping; Lu, Yubei; Yu, Xiaoyan; Chen, Shiwang; Wu, Zhengquan; Xu, Zhiyong; Kang, Wanrong

doi:10.1039/c9fo00590k

Cited by 47 publications

(25 citation statements)

References 29 publications

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“…Job's-tears (Coix lacryma-jobi L.) is an herbal plant species (Poaceae), native from Southeast Asia and used in traditional Chinese medicine. Besides its use in medicine due to the bioactive components, C. lacrymal-jobi arose as a potential staple crop to improve food security as an alternative to rice (Ruminta and Wicaksono 2017), due to high content of polysaccharides, proteins, lipids, polyphenols and phytosterols in the seeds (Li et al 2019). In addition, this plant can be used for alcoholic beverages (Wang et al 2012;Corke et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Drought-induced stress in leaves of Coix lacryma-jobi L. under exogenous application of proline and GABA amino acids

et al. 2020

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Coix lacryma-jobi L. is an Asian plant widely used in traditional medicine. Drought conditions can lead to an impairment of photosynthesis which can be softened by the exogenous application of proline and γ-aminobutyric acid (GABA) to maintain the electron transport chain (ETC) and avoid reactive oxygen species-induced oxidative damage by inducing antioxidative enzymes. The aim of this study was to evaluate the effects of water deficit on C. lacryma-jobi leaves under the application of proline and GABA. Chlorophyll (Chl) a fluorescence emission, antioxidative enzyme activity (catalase, superoxide dismutase, ascorbate peroxidase and guaiacol peroxidase) and proline and GABA contents in leaf tissues were performed in plants subjected to 12 days of drought. Chl a fluorescence indicated impairments on photosynthetic apparatus due to water deficit that resulted into increased activity of antioxidant enzymes. The application of proline but not GABA was more efficient in alleviating the effects of water deficit, as evidenced by the maintenance of the dynamic dissipation of the photosynthetic energy, besides the greater accumulation of proline under these conditions. Water deficit induces alterations in the ETC, which is softened by the application of proline to cope with the deleterious effects of drought in C. lacryma-jobi plant leaves.

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Section: Introductionmentioning

confidence: 99%

Drought-induced stress in leaves of Coix lacryma-jobi L. under exogenous application of proline and GABA amino acids

et al. 2020

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“…Therefore, the development of effective and safe therapeutic agents for inflammatory bowel diseases is urgently needed. A growing body of research suggests that natural produces derived from edible fruit and food, such as flavonoids and polysaccharides, are potential agents for inflammatory bowel diseases treatment [16,24,25].…”

Section: Discussionmentioning

confidence: 99%

“…An increasing number of studies indicate that excessive production of inflammatory cytokines induces intestinal epithelial barrier dysfunction and eventually results in impairment of TJs protein integrity [24,29]. Additionally, the imbalance of proinflammatory factors, including IL-6, IL-8, and IL-1b, is involved in the progression of the gut inflammatory cascade [24,30]. TJs proteins play an important role in preserving intestinal epithelial barrier integrity by effectively sealing the paracellular pathway of Caco-2 cells [31].…”

Section: Discussionmentioning

confidence: 99%

Total Flavonoid Extract from Hawthorn (Crataegus pinnatifida) Improves Inflammatory Cytokines-Evoked Epithelial Barrier Deficit

Liu

2020

Med Sci Monit

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Departmental sources Background: Intestinal epithelial barrier dysfunction is involved in the development and pathogenesis of intestinal diseases, such as irritable bowel syndrome, inflammatory bowel disease, and celiac disease. This study was performed to evaluate the ability of total flavonoid extract from hawthorn (TFH) to improve TNF-a-evoked intestinal epithelial barrier deficit. Material/Methods: Caco-2 cells monolayers were exposed to TNF-a in different concentrations of TFH. Intestinal epithelial barrier function was evaluated using epithelial permeability and transepithelial electrical resistance (TER). Results: Our findings showed that TFH alleviated the increase of paracellular permeability and the decline of transepithelial electrical resistance (TER) evoked by TNF-a. Additionally, 24-h pre-incubation with TFH inhibited TNF-a-evoked secretion of pro-inflammatory factors (IL-6, IL-8, MCP-1, and IL-1b). Furthermore, TFH inhibited TNF-a-evoked overexpression of pMLC and MLCK and alleviated breakdown of TJs protein (ZO-1 and occludin). The activations of Elk-1 and NFkBp65 were inhibited by TFH pre-incubation. Conclusions: TFH can alleviate TNF-a-evoked intestinal epithelial barrier deficit via the NFkBp65-mediated MLCK-MLC signaling pathway.

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“…It is not only a commonly used TCM, but it is also a commonly consumed food. Coicis semen has been proven to have numerous functions, such as detoxification and dampness and arthralgia removal; it also reduces cancer risk ( Yu et al, 2011 ; Bai et al, 2018 ; Duan, 2018 ; Xu et al, 2018 ; Choi et al, 2019 ; Huang Q. et al, 2019 ; Huang Y. L. et al, 2019 ; Li et al, 2019 ; Liu H. Q. et al, 2019 ; Liu Y. N. et al, 2019 ; Manosroi et al, 2019 ; Qian et al, 2019 ; Zhang et al, 2019 ). Moreover, it has a wide range of anti-inflammatory, antioxidation, analgesic, and sedative effects, as well as pharmacological effects against gastric cancer, hepatocellular cancer, Lewis lung cancer, non-small cell lung cancer, pancreatic cancer, and pulmonary cancer (“Fuzheng” category represented by coicis semen oil) ( Manosroi et al, 2016a ; Manosroi et al, 2016b ; Qian et al, 2016 ; Xi et al, 2016 ; Wang et al, 2016 ; Han et al, 2017 ; Qu et al, 2017a ; Schwartzberg et al, 2017 ; Jang et al, 2018 ; Kim et al, 2018 ; Zhang et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Determination of Differentiating Markers in Coicis Semen From Multi-Sources Based on Structural Similarity Classification Coupled With UPCC-Xevo G2-XS QTOF

Zhu

Shan

et al. 2020

Front. Pharmacol.

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Coicis semen, a medicinal food, is derived from the dried and mature seeds of Coix lacryma-jobi L. var. ma-yuen (Rom.Caill.) Stapf, a member of the Gramineae family. Lipids are its main constituents. Previous literature reported that coicis semen contains twenty triglycerides and twelve diglycerides. However, we identified thirty-five triglycerides, sixteen diglycerides, four monoglycerides, and two sterols under the preoptimized conditions of UPCC-Xevo G2-XS QTOF combined with a personalized TCM database. Furthermore, we successfully determined glycerol trioleate content to evaluate quality differences. Finally, we identified the fatty acid compositions of seven out of nine differential markers via Progenesis QI using principal component analysis, orthogonal projection to latent structures-discriminant analysis, and the LipidMaps database. In addition, we applied a software-based classification, a method that was previously developed by our team, to verify and predict structurally similar compounds. Our findings confirmed that UPCC-Xevo G2-XS QTOF combined with software-based group classification could be used as an efficient method for exploring the potential lipid markers of seed medicine.

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Total polysaccharides of adlay bran (Coix lachryma-jobiL.) improve TNF-α induced epithelial barrier dysfunction in Caco-2 cellsviainhibition of the inflammatory response

Abstract: Dysfunction of the intestinal epithelial barrier plays an important role in the pathogenesis of several intestinal diseases, including celiac disease, inflammatory bowel disease, and irritable bowel syndrome.

Cited by 47 publications

References 29 publications

Drought-induced stress in leaves of Coix lacryma-jobi L. under exogenous application of proline and GABA amino acids

Drought-induced stress in leaves of Coix lacryma-jobi L. under exogenous application of proline and GABA amino acids

Total Flavonoid Extract from Hawthorn (Crataegus pinnatifida) Improves Inflammatory Cytokines-Evoked Epithelial Barrier Deficit

Determination of Differentiating Markers in Coicis Semen From Multi-Sources Based on Structural Similarity Classification Coupled With UPCC-Xevo G2-XS QTOF

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