Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius

Molecular markers ISSR were used to analyse γ-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid; tested six microsatellite -primed PCR markers. First analysis of gamma-irradiated amaranth mutant lines was done using the ISSR. In the study, primers that distinguish the mutant lines from control plant were reported as well as primer with the differentiation ability for all analysed mutant lines. The very specific changes in the mutant lines´ non-coding regions based on inter microsatellite length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0.16 and their ISSR profiles were more similar to the Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0.22 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different ISSR fingerprints patters of the mutant lines when compared to the Ficha cultivar and K-433 hybrid ISSR specific profiles may be part of a consequence of the complex response of the intergenic space of mutant lines to the gamma-radiance.

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Using of Inter Microsatellite Polymorphism to evaluate gamma-irradiated<br>Amaranth mutants

iarovsk¹,

Labajov²

2013

“…Unlike the morphological and biochemical analysis, molecular characterization of yacon showed to be more efficient (Milella et al, 2011). In fact molecular markers as random amplified polymorphic DNA (RAPD) and amplified fragment length (AFLP) were presented as suitable methods for the analysis of intraspecific genetic variability in Smallanthus sonchifolius (Mansilla et al, 2006;Milella et al, 2007).…”

Section: Yacon Was Originally Cultivated In Southmentioning

confidence: 99%

“…H. Robinson -Asteraceae] is a perennial herb, originating in the Andean region. Recently, greater attention has been focused on this plant due to its agronomical, nutritional and pharmacological characteristics (Valentová et al, 2001;Milella et al, 2011). This plant produces tuberous roots rich in inulin.…”

Section: Introductionmentioning

confidence: 99%

Difference on ITS regions among Yacon genotypes and Smallanthus spp.

Žiarovská¹,

Cusimamani²,

Milella³

2014

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its medicinal properties. Yacon genetic profiles have been slightly studied using molecular markers. The aim of this study was to prepare the protocol for direct sequencing of the ITS (Internal Transcribed Spacers) regions of yacon DNA genome. The identified ITS regions in three Bolivian yacon's landraces (BOL 20, BOL 22 and BOL 24) were sequenced and then compared with ITS regions found in NCBI database, previously isolated in yacon and in other plants belonging to the genus Smallanthus. The analysed ITS regions of studied Bolivian landraces showed differences in the nucleotides 163-164 and 235-236 with the sequence previously isolated in yacon. A dendrogram was constructed by comparing the sequence presented in the manuscript and those in the NCBI database for Smallanthus, spp. In the dendrogram, the previously reported ITS sequence of Smallanthus sonchifolius was also used. Specific sites of Smallanthus sonchifolius ITS region gives a promising base for molecular approach identification of this underutilized crop.

“…PAs can protect ruminants against pasture bloat (McMahon et al, 2000) and act as antioxidants with beneficial effects for human health including protection against free radical-mediated injury and cardiovascular disease (Bagchi et al, 2000;Lin et al, 2002;Milella et al, 2011a;Russo et al, 2012). Polyphenols were also used as marker in genomic and metabolomic comparision (Milella et al, 2011b) In red wine, PAs play an important role in wine quality, contributing to mouthfeel and colour stability (Glories, 1988). Thus, from both a nutraceutical and a food quality perspective, it is important to understand the mechanisms leading to the formation of PA polymers and how this is regulated by the plant (Bogs et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Isolation of putative LAR (leucoanthocyanidin reductase) gene fragment<br>differentially expressed in Jatropha curcas L. leaves

Calabrone¹,

Milellabr²,

Martelli³

2013

Jatropha curcas Linn. (Euphorbiaceae) is a shrub, which is widely distributed especially in tropical areas of Africa. Compounds that have been isolated from J. curcas leaves include flavonoids, sterols their glucosides and alkaloids. Many of both structural and regulatory genes included in secondary metabolite pathways have been cloned from several model plants. Leaves at four different growing stages were collected and cDNAs obtained were analyzed by PCR applying specific primers. All the reactions were performed three times and only repeatable fragments were taken into account for sequencing. Obtained sequences were compared in EMBL databases in order to identify their homology and their putative function. A total of 45 polymorphic fragments were collected. Twenty-six of them showed homology with ESTs (Expressed Sequence Tagged), while other 19 sequences showed to be homologous to genes involved in secondary metabolism and polyphenol pathways. The results obtained underline a different kinetic expression of the gene fragment isolated. They are especially expressed at different growing stage. In particular, applying LAR gene specific primers, an amplicon of 945bp was obtained. It is 98.4% homologous to the gene previously isolated in V. vinifera leaves. These interesting preliminary results are the first step for study and isolate other genes involved in polyphenol pathway in Jatropha curcas.