Difference on ITS regions among Yacon genotypes and Smallanthus spp.

Žiarovská, Jana; Cusimamani, Eloy Fernández; Milella, Daniela

doi:10.9755/ejfa.v26i1.16667

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Using the bioinformatic methods, all existing sequences data can be studied for their variability in a very comfort manner. This approach is applied for both, nucleic acids or protein sequences widely (SCHVOERER et al, 2013;ŽIAROVSKÁ et al, 2014) and the results of in silico applications have the potential to became a very effective part of all standartly used molecular markers based methods.…”

Section: Resultsmentioning

confidence: 99%

Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment

2016

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Bioinformatic tool have became an inevitable part of molecular genetic research in many applications. In the present study, an in silico based approach was used to find conservative region of currently known prosystemin gene sequences and its PCR identification was performed in Amaranthus cruentus and Amaranthus hypochondriacus x hybridus. Identification results were veryfied by direct sequencing of obtained amplicons. For both of analysed species, the prosystemin sequence specifity was confirmed by sequencing. For a hybrid A. hypochondriacus x hybridus no differences were returned in bioinformatic alighment for known prosystemin sequence. For A. cruentus, two bases were found as to be different in the DNA sequence of prosystemin.

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Section: Resultsmentioning

confidence: 99%

Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment

2016

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Smallanthus sonchifolius (yacón)

2022

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Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Žiarovská¹,

Rajchl²,

Lojka³

et al. 2016

Czech J. Food Sci.

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AbstractŽiarovská J., Rajchl A., Fernández E., Prchalová J., Milella L. (2016): Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods. Czech J. Food Sci., 34: 495-502.The identification of yacon, a medicinal plant, in tea mixtures by rapid Polymerase Chain Reaction (PCR) and the Direct Analysis in Real Time coupled with Time-of-Flight Mass Spectrometry (DART/TOF-MS) method were evaluated. Three tea products and a pure yacon tea were analysed using the molecular method PCR, concretely the intraspecific variation of the internal transcribed spacer (ITS) regions of rDNA and the DART method coupled with TOF-MS. The results show the reliability of PCR and restriction cleavage of the ITS as a combined approach to confirm the presence of yacon in herbal tea mixtures. Three fragments of approximately 700, 408, and 235 bp in length are present when yacon is a part of the herbal tea mixture. The Principal Component Analysis (PCA) based on the fingerprints of the complete Total Ion Current (TIC) mass spectra shows sufficient separation of herbal teas with and without yacon leaves. The reported methods are technically rapid and can be used as an effective tool for the purposes of yacon identification or authentication.

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Difference on ITS regions among Yacon genotypes and Smallanthus spp.

Cited by 3 publications

References 22 publications

Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment

Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment

Smallanthus sonchifolius (yacón)

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

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