Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment

Abstract

Bioinformatic tool have became an inevitable part of molecular genetic research in many applications. In the present study, an in silico based approach was used to find conservative region of currently known prosystemin gene sequences and its PCR identification was performed in Amaranthus cruentus and Amaranthus hypochondriacus x hybridus. Identification results were veryfied by direct sequencing of obtained amplicons. For both of analysed species, the prosystemin sequence specifity was confirmed by sequencing. For a hybrid A. hypochondriacus x hybridus no differences were returned in bioinformatic alighment for known prosystemin sequence. For A. cruentus, two bases were found as to be different in the DNA sequence of prosystemin.