Total phenolic and flavonoid contents of n-hexane fraction in binjai leaves (Mangifera caesia Jack. ex. Wall)

Abstract

Background: Binjai leaves (Mangifera caesia Jack. ex. Wall) or M. caesia contain phenolic and flavonoid compounds important in medicinal plants development. The solvent difference and fractionation will give various active substance concentrations. Objective: To evaluate the phenolic and flavonoid content qualitatively and quantitatively from the n-hexane fraction of binjai leaves methanol extract. Methods: The soxhletation method with methanol was used to extract binjai leaves followed by fractionation. Thin Layer Chromatography (TLC) followed by spraying reagents were used to test phenols and flavonoids compounds. Folin-Ciocalteu reagents were used to analyzed total phenolics, while AlCl3 reagents were used to quantify the total flavonoid content. Result: This study gave positive results on the qualitative test of phenolics and flavonoids. Meanwhile, the fraction assay results showed total phenolic levels of 45.19 µgGAE/mg and total flavonoid levels of 165.06 µgQE/mg. Conclusion: The n-hexane fraction of M. caesia leaves methanol extracts contains phenols and flavonoid content so that it has the potential as a medicinal plant. Keywords: Binjai leaves, methanol extracts, n-hexane fraction, phenol, flavonoid

Uji Aktivitas Antioksidan Fraksi N-Heksan dari Ekstrak Metanol Daun Binjai Mangifera caesia Jack. Ex. Wall Menggunakan Metode DPPH

“…Penelitian Yuliani et al juga menyebutkan kadar total fenolik yang berperan dalam menghasilkan aktivitas antioksidan pada fraksi n-heksan ekstrak metanol daun binjai yaitu 45,2 μgGAE/mg fraksi yang tergolong lebih kecil jika dibandingkan fraksi etil asetat (430 μgGAE/mg fraksi) dan fraksi airnya (782 μgGAE/mg fraksi) (27) . Akan tetapi, kadar total fl avonoid yang dihasilkan oleh fraksi n-heksan ini (165,06 μgQE/mg fraksi) lebih tinggi dibandingkan fraksi airnya (118,8 μgQE/mg fraksi), walaupun masih tergolong lebih rendah jika dibandingkan fraksi etil asetatnya (274,32 μgQE/mg fraksi) (7,27) . Hasil ini menunjukkan bahwa kandungan senyawa fl avonoid dalam ekstrak metanol daun binjai mayoritas memiliki kepolaran yang cukup rendah atau bersifat semi polar sampai non polar (10) .…”