Total phenolic and flavonoid content, antioxidant and antimicrobial activity of extracts from Tordylium maximum

Abstract

The present study describes the total phenolic and flavonoid content and in vitro antioxidant and antimicrobial activity of methanol and water extracts from Tordylium maximum L. wild growing in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 74.59 to 83.36 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 20.48 to 47.71 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 4.042 to 7.825 IC50 (mg/ml) and from 2.48 to 2.78 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay against the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that T. maximum may be considered as a natural source of antioxidants and antimicrobial agents.

Characterization of Cistus × incanus L. and Cistus ladanifer L. Extracts as Potential Multifunctional Antioxidant Ingredients for Skin Protecting Cosmetics

“…The concentration of flavonoids in Cistus extracts was measured according to Matejić et al protocol [21] with some modifications. Briefly, 150 µL of dissolved extracts (1mg/mL) were mixed with 650 µL reaction mixture (61.5 mL 80% C 2 H 5 OH + 1.5 mL 10% Al(NO 3 ) 3 ·9H 2 O + 1.5 mL 1 M CH 3 COOK).…”

Galenic formulations of Cannabis sativa : comparison of the chemical properties of extracts obtained by simple protocols using lipidic vehicles

“…Total flavonoid content was determined using aluminum nitrate nonahydrate (AlCl3) according to the procedure reported by Matejic et al with some modifications (Matejić et al 2013). To this end, 100 L of cannabis oils (or the oily vehicle alone, used as control), mixed with 100 L of AlCl3 2% w/v dissolved in a solution of 95% methanol and 5% acetic acid, was added.…”

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“…Determination of the antioxidant capacity was determined by the DPPH method, i.e. neutralization of the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical according to the method described in the work by Matejić et al (2013) with minimal changes [8].…”