Total Glycosides of Peony Protects Against Inflammatory Bowel Disease by Regulating IL-23/IL-17 Axis and Th17/Treg Balance

Abstract

Inflammatory bowel disease (IBD) is a group of autoimmune diseases, including ulcerative colitis and Crohn’s disease, characterized by nonspecific inflammation in the gut. Total glycoside of peony (TGP) has been widely used for treatment of autoimmune diseases because of its pharmacological effects. However, it is lack of depth in whether TGP regulate T helper 17 cell (Th17) / T regulatory cell (Treg) immune balance or interleukin 23 (IL-23) / IL-17 axis to achieve the goal of treating IBD. Hence, the aim of this study was to investigate the effects of TGP on experimental colitis mice and the related mechanisms. In the present study, we demonstrated that administration of TGP effectively attenuates colonic inflammation of TNBS-induced colitis mice, mainly reflected in significantly improved clinical parameters, reduced inflammatory response and myeloperoxidase (MPO) activity, even stronger systemic immune ability and effective improvement of Th17/Treg immune disorders. In addition, there was a stronger immunosuppressive ability in a positive cluster of differentiation 4 (CD4[Formula: see text]) T-lymphocytes from the TGP treated mouse colon, characterized by the inhibition of high levels of inflammatory factors and increased regulatory T cells. Importantly, high-dose TGP has similar therapeutic effects as salicylazosulfapyridine (SASP) on IBD treatment. The potential mechanisms might be, at least in part, related to the adjustment of imbalance of Th17/Treg cells and the inhibition of IL-23/IL17 inflammatory signal axis.

Lactobacillus Paracasei R3 Protects Against Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice via Regulating Th17/Treg Cell Balance

“…It is generally believed that the pathogenesis of UC involves the results of multiple interactions including genetics, microorganisms, and the body's immune system [20]. In recent years, the pathogenesis of UC has been continuously studied through UC patients and animal models of UC, and it has been found that the occurrence and development of UC are closely related to the balance of Foxp3 + Tregs cells and Th17 cells (producing IL-17A and IL-17F)in vivo [21][22][23].…”

“…participate in the pathogenesis of UC by regulating the release of inhibitory cytokines (IL-10, etc. ), and their low number or dysfunction can lead to the occurrence of the disease [23][24][25]. T helper cell 17(Th17) is a subset of T cells capable of secreting IL-17.…”

The association between IL‐17A and IL‐23R polymorphisms and coronary artery disease risk in a Middle Eastern Chinese population

“…22,23 In addition, IL-23/IL-23R polymorphisms are involved in pathogenesis of CAD by regulating the immune system. 24 A previous study showed that the IL-23/IL-17 inflammatory signal could adjust the balance of Th17/Treg cells, 25 a process which was related to the progression of CAD. 26 Moreover, the IL-23 level was suggested to be a marker of patients with CAD.…”

Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/β-catenin signaling pathway

“…Next, the membranes were incubated with HRP-conjugated goat anti rabbit secondary antibody (1:10000) for 1 h at room temperature. At last, images were obtained from multifunctional Gel Imaging System (Image Quant LAS 500, General Electric, Fairfield, CT, USA), and the gray value of each band was quantified and analyzed using IQTL 8.1 software [23].…”