Total, free, and protein-bound thiols in plasma of peritoneal dialysis and predialysis patients

Przemysław, Włodek; Ksiazek, Piotr; Chwatko, Grażyna; Kowalczyk-Pachel, Danuta; Iciek, Małgorzata; Marcykiewicz, Bernadeta; Suliga, Małgorzata; Witold, Smoleński

doi:10.1007/s11255-011-9905-1

Cited by 13 publications

(11 citation statements)

References 21 publications

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“…These results indicate that the level of oxidative stress is higher in patients with ESRD than in healthy individuals. Our findings are consistent with previous reports wherein patients with ESRD have been shown to have decreased plasma protein thiols and increased S-thiolated proteins (9,13,14). In addition, Przemysław et al (14) found that the total concentration of glutathione, which is a low molecular weight thiol-tripeptide, was decreased in terminal renal failure patients and that total concentrations of the remaining thiols in these patients were significantly increased compared to those in the healthy subjects.…”

Section: Discussionsupporting

confidence: 93%

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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OBJECTIVE:We aimed to evaluate thiol/disulfide homeostasis as an oxidative stress parameter in end-stage renal disease patients receiving different substitutive therapies. MATERIAL and METHODS:Twenty-four renal transplantation (46.2±8.1 years), 24 peritoneal dialysis (47.8±8.5 years), and 25 hemodialysis patients (45.7±16.0 years), and 24 healthy controls (47.6±8.1 years) were included. Serum native thiol, total thiol, and disulfide levels were measured for all subjects. RESULTS:Serum total thiol and native thiol levels in the ESRD patients were significantly lower than those in the controls (P<0.001). Native and total thiol levels were significantly lower in PD (256 ± 45 μmol/L, and 287 ± 57 μmol/L) compared to HD (324 ± 44 μmol/L and 356 ± 46 μmol/L) group (p<0.01 and p=0.03). Disulfide/native thiol and disulfide/total thiol levels increased significantly in the PD and transplantation patients compared to control group (P<0.001). Positive correlation was observed between total thiols (r= 0.491 P<0.001), disulfide/native thiol ratio (r=0.383, P<0.001), and blood urea nitrogen; disulfide levels and blood urea nitrogen showed a negative correlation (-0.415, P<0.001). Disulfide levels and glomerular filtration rate showed a positive correlation (r=0.276, P=0.030). CONCLUSION:Serum thiol/disulfide homeostasis can be an indicator of oxidative stress in end-stage renal disease and that thiol/disulfide homeostasis analysis should be included in the routine monitoring of end-stage renal disease patients. KEy wORDS: Hemodialysis, Peritoneal dialysis, Renal transplantationÖz AMAÇ: Çalışmada son dönem böbrek yetmezliği hastalığı için farklı renal replasman tedavisi alan hastalarda bir oksidatif stres belirteci olarak tiol/disülfit dengesinin araştırılması amaçlanmıştır. GEREÇ ve yÖNTEMLER:Yirmi dört böbrek nakli yapılmış (46,2±8,1 yaş), 25 hemodiyaliz (45,7±16,0 yaş) ve 24 periton diyalizi (47,8±8,5 yaş) hastaları ile 24 sağlıklı kontrol (47,6±8,1 yaş) çalışmaya dahil edildi. Tüm olgularda nativ tiyol, total tiyol ve disülfit seviyeleri ölçüldü. BULGULAR:Hastalarda kontrol grubu ile karşılaştırıldığında total tiyol ve nativ tiyol seviyeleri anlamlı olarak düşüktü (p<0,001). Nativ ve total tiyol düzeyi periton diyalizi hastalarında (256±45 μmol/L ve 287±57 μmol/L) hemodiyaliz hastalarına (324±44 μmol/L ve 356±46 μmol/L) göre anlamlı düşüktü (p<0,01 ve p=0,03). Periton diyalizi ve transplantasyon hastalarında disulfit/nativ tiyol ve disülfit/total tiyol düzeyleri kontrol grubuna gore anlamlı olarak yüksek bulundu (p<0,001). Total tiyol (r=0,491 P<0,001) ve disulfit/nativ tiyol oranı (r=0,383, P<0,001) ile kan üre nitrojeni arasında pozitif korelasyon; disülfit seviyeleri ve kan üre nitrojeni arasında negatif korelasyon (-0,415, P<0,001) saptandı. Disülfit seviyeleri ve glomerülar filtrasyon değeri arasında pozitif korelasyon (r=0,276, P=0,030) bulundu. SONUÇ:Tedavi alan son dönem böbrek hastalarında rutin hasta takibinde serum tiyol/disülfit oranı oksidatif stres belirteci olarak kullanılabilir.

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Section: Discussionsupporting

confidence: 93%

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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“…Decreased plasma protein thiols and increased S-thiolated proteins have previously been shown in end-stage renal disease patients (13)(14)(15).…”

Section: Introductionmentioning

confidence: 89%

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Esen¹,

Kahvecioğlu²,

Üstündağ³

et al. 2018

Turk Neph Dial Transpl

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OBJECTIVE:The aim of this study was to determine a novel oxidative stress marker, plasma thioldisulphide homoeostasis, in patients with autosomal dominant polycystic kidney disease (APKD). MATERIAL and METHODS:Thirty-four patients with autosomal dominant polycystic kidney disease and 30 healthy volunteers were included in the study. The thiol-disulfide homeostasis assay was performed. Total antioxidant capacity was evaluated via the FRAP assay. RESULTS:The median age was 46 (13) years in the patient group and 41 (13) years in the control group. In the APKD disease group, serum total and native thiol levels were statistically significantly lower (p = 0.002, p = 0.002, respectively). Serum native thiol levels were negatively correlated with age (r:-0.620 p: 0,000), systolic blood pressure (r: -0,697 p: 0,000), and diastolic blood pressure (r: -0,643 p: 0,000). A weak positive correlation was found between the disulfide/native thiol ratio and creatinine (r: 0.564 p <0.001), and a negative correlation was found between the disulfide/native thiol ratio and glomerular filtration rate (r: -0.372 p: 0.030). There was a significant positive correlation between the native thiol level and GFR (r: 0.699, p <0.001) level. CONCLUSION:Our findings suggest that thiol/disulfide homeostasis may be associated with autosomal dominant polycystic kidney disease progression. KEY WORDS:Oxidative stress, Autosomal dominant polycystic kidney disease, Thiol-disulphide, Creatinine ÖZ AMAÇ: Bu çalışmanın amacı, otozomal dominant polikistik böbrek (ODPK) hastalığı olanlarda yeni bir oksidatif stres markeri olan plazma tiyol-disülfid homeostazisini belirlemektir. GEREÇ ve YÖNTEMLER:Otozomal dominant polikistik böbrek hastalığı olan 34 hasta ve 30 sağlıklı gönüllü çalışmaya dahil edildi. Tiyol-disülfid homeostazı ölçüldü. Total antioksidan kapasite FRAP testi ile değerlendirildi. BULGULAR:Hastaların medyan yaşı 46 (13) yıl, kontrol grubunda 41 (13) yıldır. ODPK hastalarında serum total ve nativ tiyol düzeyleri istatistiksel olarak anlamlı derecede düşüktü (sırasıyla p=0,002, p=0,002). Serum nativ tiyol düzeyleri yaş (r:-0,620 p:0,000), sistolik kan basıncı (r:-0,697 p:0,000) ve diyastolik kan basıncıyla (r:-0,643 p:0,000) negatif korelasyon gösterdi. Disülfid / nativ tiyol oranı ile kreatinin arasında (r:0,564 p <0,001) zayıf pozitif korelasyon bulundu, disülfid / nativ tiyol oranı ve glomerüler filtrasyon hızı arasında negatif bir korelasyon bulundu (r:-0,372, p:0,030. Nativ tiyol düzeyi ile GFR arasında pozitif korelasyon bulundu (r:0,699, p<0,001). SONUÇ:Bulgularımız tiyol/disülfid homeostazının otozomal dominant polikistik böbrek hastalığının ilerlemesi ile ilişkili olabileceğini düşündürmektedir.

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“…Mounting evidence has shown that perturbations in the thiol/ disulfide homeostasis and an oxidative shift in the thiol/disulfide redox potential in plasma are associated with aging and with several different diseases, such as atherosclerosis, chronic renal failure, and preeclampsia [14][15][16][17][18]. However, the complex dynamics of thiol redox status in regulating protein function and gene expression as well as the causal role of thiol dysregulation in disease pathogenesis remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Protein thiolation index (PTI) as a biomarker of oxidative stress

Giustarini

Dalle‐Donne

Lorenzini

et al. 2012

Free Radical Biology and Medicine

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Total, free, and protein-bound thiols in plasma of peritoneal dialysis and predialysis patients

Cited by 13 publications

References 21 publications

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Protein thiolation index (PTI) as a biomarker of oxidative stress

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