Total antioxidant potential of resinous exudates from<i>Heliotropium</i>species, and a comparison of the ABTS and DPPH methods

Lissi, E. A.; Modak, Brenda; Torres, René; Escobar, Jorge; Urzúa, Alejandro

doi:10.1080/10715769900300511

Cited by 242 publications

(164 citation statements)

References 21 publications

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“…Extracts obtained at 40 min had a greater scavenging capacity for DPPH radicals. This may be due to the antioxidant compounds in the extracts obtained at different times showed differences by employing DPPH and ABTS scavenging assays, which was similar with previous reports [34][35][36]. …”

Section: Effects Of Different Extraction Timesupporting

confidence: 80%

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

2015

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Ultrasonic-assisted extraction (UAE) was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology (RSM), based on a four-factor, five-level central composite design (CCD), was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) methods. The results showed good fit with the proposed models for the total flavonoid extraction (R 2 = 0.9849), for the antioxidant extraction assayed by ABTS method (R 2 = 0.9764), and by DPPH method (R 2 = 0.9806). Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL/g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL/g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL/g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa.

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Section: Effects Of Different Extraction Timesupporting

confidence: 80%

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

2015

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“…Blank sample was run without ABTS and using MeOH/phosphate buffer (1:1) instead of a sample. Negative control was run with ABTS and MeOH/phosphate buffer (1:1) instead of test compound (18)(19)(20).…”

Section: Pharmacological Screeningmentioning

confidence: 99%

Design and synthesis of novel thiophenecarbohydrazide, thienopyrazole and thienopyrimidine derivatives as antioxidant and antitumor agents

Abu‐Hashem¹,

Shehry²,

Badria³

2010

Acta Pharmaceutica

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The ultimate position of substituted 2-aminothiophenes in the field of drug design and synthesis of pharmaceuticals comes from their advantageous properties. The thiophene ring is bioisosteric replacement for the phenyl group broadly present in active drugs; the thiophene core exists in many natural and synthetic pharmaceuticals (1, 2). In the present work, ethyl-2-aminothiophene-3-carboxylic was expressed as a synthone for many new condensed heterocyclic systems viz. thieno [2,3-d] 2-Amino-5-acetyl-4-methyl-thiophene-3-carboxylic acid ethyl ester (1) and 5-acetyl-2-amino-4-methylthiophene--3-carbohydrazide (2) were synthesized and used as starting materials for the synthesis of new series of 1-pyrimidin-4(3H)-one (10) and 5-methyl-6-acetyl-2--thioxo-2,3-dihydrothieno[2,3-d]pyrimidin-4(1H)-one (12) as potential antioxidant and antitumor agents. Pharmacological tests showed that compounds 6a, 6b, 8, 10 and 12 exhibited significant antitumor and antioxidant activity.

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“…This incomplete consumption is explainable both in terms of a slow initial step and/or to the reversibility of the secondary reactions when compounds of low reactivity are considered. 42 …”

Section: Discussionmentioning

confidence: 99%

Kinetic and Stoichiometric Evaluation of Free Radicals Scavengers Activities Based on Diphenyl-Picryl Hydrazyyl (Dpph) Consumption

Campos

Duran

López-Alarcón

et al. 2012

J. Chil. Chem. Soc.

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Kinetics and stoichiometric measurements based on the reactions of stable free radicals are among the most employed procedures in the evaluation of antioxidant activities of pure compounds and complex mixtures. In the present work it is shown that initial rates of diphenyl-picryl hydrazyl (DPPH) consumption elicited by cinnamic acid derivatives are closely related to their reactivity towards peroxyl radicals. This implies that kinetic data regarding DPPH bleaching can be useful on the evaluation of antioxidant reactivities. On the other hand, data obtained at a single (long) reaction time are unrelated to the reactivity of the antioxidants and mostly reflect the number of phenol groups present in the tested sample

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Total antioxidant potential of resinous exudates fromHeliotropiumspecies, and a comparison of the ABTS and DPPH methods

Cited by 242 publications

References 21 publications

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

Design and synthesis of novel thiophenecarbohydrazide, thienopyrazole and thienopyrimidine derivatives as antioxidant and antitumor agents

Kinetic and Stoichiometric Evaluation of Free Radicals Scavengers Activities Based on Diphenyl-Picryl Hydrazyyl (Dpph) Consumption

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