Total and epidermal cyclic AMP levels related to the variations of ecdysteroids and bursicon during the metamorphosis of the mealworm Tenebrio molitor L.

Delachambre, Jean; Delbecque, Jean‐Paul; Provansal, A.; Grillot, J.-P.; Reggi, M.L. De; Cailla, H.

doi:10.1016/0020-1790(79)90032-5

Cited by 20 publications

(2 citation statements)

References 28 publications

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“…MartinDiaz et al (2009) demonstrated that carbamazepine reduced cyclic AMP levels and Protein Kinase A (PKA) activities in glands, gills and mantle in the mussel Mytilus galloprovincialis. In insects cyclic AMP as a secondary messenger for molting hormones including ecdysone, eclosion hormone and bursicon (Delachambre et al 1979;Smith et al 1984;Gilbert et al 2002;Rewitz et al 2009). Therefore, it has been suggested that carbamazepine influences the synthesis and bioavailability of ecdysone and alter ecdysis of aquatic insects as seen by Oetken et al (2005) and Nentwig et al (2004).…”

Section: Possible Chemical Mode Of Action Of Carbamazepinementioning

confidence: 99%

The effects of the pharmaceutical carbamazepine on life history characteristics of flat-headed mayflies (Heptageniidae) and aquatic resource interactions

Jarvis

Bernot

2014

Ecotoxicology

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Pharmaceutical pollutants are commonly detected in freshwater ecosystems around the world and have biological effects on aquatic organisms. However, current understanding of the influence this contaminant class has on freshwater communities and ecosystems is lacking. Recently the scientific community has called for research focusing on certain pharmaceuticals due to their ubiquity and potential toxicity. Carbamazepine is one of these pharmaceuticals. To better understand the effect carbamazepine has on life history characteristics of aquatic organisms and consumer-resource interactions, we quantified the influence of carbamazepine on the development, growth and behavior of mayfly nymphs (Stenonema sp.) and the alterations in food consumer-resource interactions between Stenonema and algae (Chaetophora). Microcosms were assembled in a factorial design containing algae and mayfly nymphs native to central Indiana and dosed with environmentally relevant concentrations of carbamazepine. From this ecotoxicological experiment we were able to infer that carbamazepine at 2,000 ng/L influenced the development and behavior of Stenonema nymphs and the body dimensions of adult individuals. However, it appears that carbamazepine does not influence consumer-resource interactions at concentrations found in surface waters. The pharmaceutical carbamazepine may influence the behavior, growth and development of mayflies, which could have significant consequences at the population, community and ecosystem level.

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Section: Possible Chemical Mode Of Action Of Carbamazepinementioning

confidence: 99%

The effects of the pharmaceutical carbamazepine on life history characteristics of flat-headed mayflies (Heptageniidae) and aquatic resource interactions

Jarvis

Bernot

2014

Ecotoxicology

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“…How bursicon then causes the tanning of the cuticle is unclear. Nevertheless, it has been assumed that it would act directly on the epidermis to cause the secretion of melanin and reactive quinones into the overlying extracellular cuticular matrix [13][14][15]. Consistent with this hypothesis, rk is expressed in epidermal cells, and rk mutants show a delay in the phosphorylation of epithelial TH, which is necessary for the activation of the tanning pathway [13].…”

Section: Introductionmentioning

confidence: 96%

The tanning hormone, bursicon, does not act directly on the epidermis to tan the Drosophila exoskeleton

et al. 2020

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Background: In insects, continuous growth requires the periodic replacement of the exoskeleton. Once the remains of the exoskeleton from the previous stage have been shed during ecdysis, the new one is rapidly sclerotized (hardened) and melanized (pigmented), a process collectively known as tanning. The rapid tanning that occurs after ecdysis is critical for insect survival, as it reduces desiccation, and gives the exoskeleton the rigidity needed to support the internal organs and to provide a solid anchor for the muscles. This rapid postecdysial tanning is triggered by the "tanning hormone", bursicon. Since bursicon is released into the hemolymph, it has naturally been assumed that it would act on the epidermal cells to cause the tanning of the overlying exoskeleton. Results: Here we investigated the site of bursicon action in Drosophila by examining the consequences on tanning of disabling the bursicon receptor (encoded by the rickets gene) in different tissues. To our surprise, we found that rapid tanning does not require rickets function in the epidermis but requires it instead in peptidergic neurons of the ventral nervous system (VNS). Although we were unable to identify the signal that is transmitted from the VNS to the epidermis, we show that neurons that express the Drosophila insulin-like peptide ILP7, but not the ILP7 peptide itself, are involved. In addition, we found that some of the bursicon targets involved in melanization are different from those that cause sclerotization. Conclusions: Our findings show that bursicon does not act directly on the epidermis to cause the tanning of the overlying exoskeleton but instead requires an intermediary messenger produced by peptidergic neurons within the central nervous system. Thus, this work has uncovered an unexpected layer of control in a process that is critical for insect survival, which will significantly alter the direction of future research aimed at understanding how rapid postecdysial tanning occurs.

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Evidence for the induction of cuticle proteins by 20‐hydroxyecdysone in two established insect cell lines

Stiles

Newman

1992

Arch Insect Biochem Physiol

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We have studied two different established insect cell lines capable of synthesizing chitin, UM-BGE-4 from the cockroach and BRL-AC-2 from the boll weevil, for their ability to produce putative cuticle proteins. Mouse polyclonal antibodies were raised against the abdominal cuticle proteins extracted from the cuticle of nymphal and adult stage cockroaches, and larval, pupal, and adult stage cotton boll weevils. O n Western blots, these antibodies detected several cross-reacting proteins in untreated control cells and a variety of induced proteins in cells first exposed to a double pulse of the molting hormone, 20-hydroxyecdysone (a low concentration followed by a higher concentration of hormone). The majority of these hormone induced proteins recognized by the anti-cuticle protein antibodies were found to correspond in size to specific proteins observed in abdominal cuticle extracts. Electron microscopy utilizing colloidal gold to identify antibody binding sites indicated the antigenic proteins were present in the extracellular material found on the surface of the cultured cockroach and boll weevil cells. The evidence presented suggests that these cell lines can produce cuticle proteins. 01992 WiIey-Liss, Inc.

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Total and epidermal cyclic AMP levels related to the variations of ecdysteroids and bursicon during the metamorphosis of the mealworm Tenebrio molitor L.

Cited by 20 publications

References 28 publications

The effects of the pharmaceutical carbamazepine on life history characteristics of flat-headed mayflies (Heptageniidae) and aquatic resource interactions

The effects of the pharmaceutical carbamazepine on life history characteristics of flat-headed mayflies (Heptageniidae) and aquatic resource interactions

The tanning hormone, bursicon, does not act directly on the epidermis to tan the Drosophila exoskeleton

Evidence for the induction of cuticle proteins by 20‐hydroxyecdysone in two established insect cell lines

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