Abstract. We define analytic torsion τ (X, E , H) ∈ det H• (X, E , H) for the twisted de Rham complex, consisting of the spaces of differential forms on a compact oriented Riemannian manifold X valued in a flat vector bundle E , with a differential given by ∇ E +H ∧ · , where ∇ E is a flat connection on E , H is an odd-degree closed differential form on X, and H• (X, E , H) denotes the cohomology of this Z2-graded complex. The definition uses pseudodifferential operators and residue traces. We show that when dim X is odd, τ (X, E , H) is independent of the choice of metrics on X and E and of the representative H in the cohomology class [H]. We define twisted analytic torsion in the context of generalized geometry and show that when H is a 3-form, the deformation H → H − dB, where B is a 2-form on X, is equivalent to deforming a usual metric g to a generalized metric (g, B). We demonstrate some basic functorial properties. When H is a top-degree form, we compute the torsion, define its simplicial counterpart and prove an analogue of the Cheeger-Müller Theorem. We also study the twisted analytic torsion for T -dual circle bundles with integral 3-form fluxes.