Topographic and phase-contrast imaging in atomic force microscopy

Pang, G.K.H.; Baba-Kishi, K. Z.; Patel, Amisha

doi:10.1016/s0304-3991(99)00164-3

Cited by 98 publications

(61 citation statements)

References 4 publications

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“…Its color code corresponds to the tapping mode phase signal, which is used to visualize material anisotropies, e.g., variations in sample hardness. [15,16] In our case, brighter regions (harder diamond crystals) are surrounded by a network of dark structures, the comparably softer grain boundaries, built up of amorphous sp 3 and sp 2 carbon. [14] Individual diamond crystallites (10-20 nm) are arranged in larger grains with diameters up to $150 nm.…”

Section: Characteristics Of Ncd-coated Glass Substratesmentioning

confidence: 97%

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

Klauser

Hermann

Steinmüller‐Nethl³

et al. 2010

Chemical Vapor Deposition

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The aim of this study is to investigate the influence of wettability on direct and protein-mediated cell-surface interactions. Nanocrystalline diamond (NCD) is chosen as a cell growth substrate, because it offers the possibility to adjust surface properties like roughness or chemical termination, thus realizing hydrophilic or hydrophobic surfaces. Cell adhesion is studied on NCD films with a defined roughness but different surface chemistries (hydrogen, fluorine, and oxygen terminations) using two different cell lines (LLC-PK1 and PANC-1). They are cultured with and without the addition of fetal bovine serum (FBS) to the growth medium in order to discriminate between direct and mediated cell adhesion. For both cell lines, the cells are not able to adhere to hydrophobic surfaces without the addition of FBS to the growth medium, whereas they can attach in the presence of FBS. Hydrophilic surfaces enable cell attachment with and without the addition of FBS to the medium.

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Section: Characteristics Of Ncd-coated Glass Substratesmentioning

confidence: 97%

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

Klauser

Hermann

Steinmüller‐Nethl³

et al. 2010

Chemical Vapor Deposition

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“…observation of protein molecules compared to conventional AFM imaging, but also provides information of surface properties of the molecules [19,20]. Consequently, phase imaging is emerging as a powerful tool for mapping variations in sample properties with superior details and with no cost in speed or resolution.…”

Section: Introductionmentioning

confidence: 99%

Imaging recognition events between human IgG and rat anti-human IgG by atomic force microscopy

Wang

Chen

et al. 2010

International Journal of Biological Macromolecules

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“…Phase images obtained simultaneously with height images give additional information of the microstructure. 20,21 CRM analysis.-Raman analysis was performed for the composite film deposited on the carbon steel as well as reference samples of Mefp-1 and ceria films, respectively. The reference samples were prepared by putting a drop of a concentrated solution of Mefp-1 or ceria onto the carbon steel surface and waiting for the solution to dry out, so that a thick layer of Mefp-1 or ceria was formed on the surface.…”

Section: Qcm With Dissipation (Qcm-d)mentioning

confidence: 99%

Thin Composite Films of Mussel Adhesive Proteins and Ceria Nanoparticles on Carbon Steel for Corrosion Protection

Sababi¹,

Zhang²,

Krivosheeva³

et al. 2012

J. Electrochem. Soc.

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Thin composite films of the mussel adhesive proteins (Mefp-1) and ceria nanoparticles were deposited on substrate surfaces by alternating immersions. The film formation was studied by quartz crystal microbalance with dissipation (QCM-D) monitoring. Both the changes in frequency and dissipation recorded by QCM-D demonstrate buildup of a composite film of Mefp-1 and nanoceria. Micro-and nanostructure and composition of the film on carbon steel were characterized by optical and atomic force microscopy (AFM), electron probe micro analyzer (EPMA) and confocal Raman microspectroscopy (CRM). Optical and EPMA observations of the film show micron-sized aggregates and AFM imaging of the compact and smooth areas reveal the nanostructure. EPMA elemental mapping indicates that the micron-sized aggregates are rich in ceria and Mefp-1, whereas CRM analysis shows the presence of Mefp-1-Fe complexes in the film. Corrosion protection of the composite film on carbon steel was investigated by electrochemical impedance spectroscopy and potentiodynamic polarization measurements in NaCl solution, and compared with Mefp-1 added in the solution as an inhibitor. The measurements show that the composite film provides a higher corrosion resistance compared with Mefp-1 added as inhibitor. The corrosion resistance increases with exposure time and approaches a high level. Corrosion inhibitors are widely used to control corrosion of metallic materials that causes huge economic cost to our society, and even catastrophes due to unexpected failure of metallic constructions and devices. In the past decade, extensive efforts have been devoted to developing new "green" corrosion inhibitors to replace traditional ones that are harmful to human health and ecological systems.1 Filmforming inhibitors based on organic substances that are more "green" for human and environment have attracted considerable attention in recent years. For example, a mussel adhesive protein (MAP), derived from marine mussel Mytilus edulis, has shown a certain inhibition effect on stainless steel.2 In our previous study, Mefp-1, the type of MAP that has been most studied, was shown to exhibit interesting inhibiting properties on carbon steel in NaCl solutions. However, the inhibition efficiency of Mefp-1 is limited since the adsorbed film is not sufficiently compact, and this is particularly a problem for the initial period during which Mefp-1 does not give a significant inhibition effect. 3The adhesive and cohesive properties of MAP are generally attributed to the high content of di-hydroxyphenylalanine (DOPA), 4 which has been reported to form complexes with metal ions 5,6 and metal oxides. Waite et al. have concluded that the DOPA moiety in the proteins serves two important roles in MAP adhesion to many surfacesthe formation of interfacial complexes promoting chemisorption and cross-links. 8,9 In another study of adsorption and corrosion protection of polydopamine based surface films on aluminum alloys, it was concluded that these self-assembled surface films can provide a c...

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Topographic and phase-contrast imaging in atomic force microscopy

Cited by 98 publications

References 4 publications

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

Imaging recognition events between human IgG and rat anti-human IgG by atomic force microscopy

Thin Composite Films of Mussel Adhesive Proteins and Ceria Nanoparticles on Carbon Steel for Corrosion Protection

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