Topically applied Melaleuca alternifolia (tea tree) oil causes direct anti-cancer cytotoxicity in subcutaneous tumour bearing mice

Ireland, Demelza J.; Greay, Sara J.; Hooper, Cornelia M.; Filion, Pierre; Riley, Thomas V.; Beilharz, Manfred W.

doi:10.1016/j.jdermsci.2012.05.005

Cited by 29 publications

(15 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TTO is a natural essential oil which is composed of several organic substances such as terpene hydrocarbons, terpene alcohols, and terpene phenols. ere are several reports regarding antimicrobial and anticancer properties of TTO [170,171]. However, like other EOs, the application of TTO remains limited due to its chemical instability in exposure to air and high temperatures [172].…”

Section: Encapsulation Of Tea Tree Oil (Tto) Peppermint Oil (Po) Chmentioning

confidence: 99%

Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging

Ataei

Azari

Hassan

et al. 2020

Advances in Polymer Technology

View full text Add to dashboard Cite

The growth of global food demand combined with the increased appeal to access different foods from every corner of the globe is forcing the food industry to look for alternative technologies to increase the shelf life. Essential oils (EOs) as naturally occurring functional ingredients have shown great prospects in active food packaging. EOs can inhibit the growth of superficial food pathogens, modify nutritious values without affecting the sensory qualities of food, and prolong the shelf life when used in food packaging as an active ingredient. Since 2016, various reports have demonstrated that combinations of electrospun fibers and encapsulated EOs could offer promising results when used as food packaging. Such electrospun platforms have encapsulated either pure EOs or their complexation with other antibacterial agents to prolong the shelf life of food products through sustained release of active ingredients. This paper presents a comprehensive review of the essential oil-loaded electrospun fibers that have been applied as active food packaging material.

show abstract

Section: Encapsulation Of Tea Tree Oil (Tto) Peppermint Oil (Po) Chmentioning

confidence: 99%

Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging

Ataei

Azari

Hassan

et al. 2020

Advances in Polymer Technology

View full text Add to dashboard Cite

show abstract

“…[8] Other studies which investigated the efficacy of topical TTO on aggressive, subcutaneous, and chemoresistant tumors in fully immune-competent mice. [3,27] The other studies have shown that topical treatment with 10% TTO, which was given once a day for 4 consecutive days, induced a significant, regression of established subcutaneous AE17 tumors and slowed the growth of B16-F10 tumors. A penetration enhancer DMSO was necessary to induce the antitumor effect; no effects were evident when using TTO or solvents other than DMSO (i.e., isopropanol or acetone).…”

Section: Discussionmentioning

confidence: 99%

“…[26] Hence, it confirms that TTO might obtain its effect by inhibiting rapidly dividing cells more readily than slower growing non-cancerous cells. [27] It is also noted that drug-sensitive and drug-resistant melanoma cells were mainly interfered with the migration and invasion processes by TTO and its major component, terpinen-4-ol. [8] Other studies which investigated the efficacy of topical TTO on aggressive, subcutaneous, and chemoresistant tumors in fully immune-competent mice.…”

Section: Discussionmentioning

confidence: 99%

“…[3] The transmission electron microscopy analysis of AE17 tumor sections from mice treated topically with TTO revealed that following in vivo TTO treatment, tumor cells undergo primary necrosis as originally suggested in vitro studies. [9,27] Conclusion AS far as potential anticancer activity of essential oils is concerned, still there is no much information available. Hence, lot of research needs to be progressed in this regard.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of anticancer activity of Melaleuca alternifolia (i.e., tea tree oil) on colon cancer cell line (HT29) - An in vitro study

Byahatti¹,

Bogar²,

Bhat³

et al. 2018

JCRI

View full text Add to dashboard Cite

Introduction and Aim: Exploring naturally available plants as a source of new therapeutic agents, in treating the different types of cancer is currently gaining a lot of importance. One such naturally available plant extract derived from Australian native plant Melaleuca alternifolia (i.e., tea tree oil [TTO]) which belongs to the family of essential oils is a very good antibacterial, antifungal, antiviral, antiprotozoal, and antiinflammatory agent. However, currently, a lot of importance is given for its anticancer effect. Hence, our aim is to evaluate anticancer activity of M. alternifolia on colon cancer cell line (HT29). Materials and Methods: Before, we started our study ethical clearance was obtained from the institutional review board. The cytotoxicity checked for colon cancer (HT29) cell line with positive control being Cisplatin by an MTT assay. MTT is an in vitro method of analysis of cytotoxicity of TTO on colon cancer cell line.TTO was obtained from crystal aromatics, New Delhi, imported from Australia with refractive index of 1.475. These cell lines which were used in our study were procured from NCCS Pune, India. (1) MTT solution preparation (stock solution): 5 mg in 1 mL of PBS. (2) Cell culture: During culture, the cell lines were maintained in 96 wells microtiter plate which contains MEM media supplemented with 10% heat-inactivated fetal calf serum (FCS), containing 5% of mixture of gentamicin (10 ug), penicillin (100 units/mL), and streptomycin (100 µg/ mL) in the presence of 5% CO 2 at 37°C for 48-72 h. (3) Cytotoxicity assay: Wherein, there is an in vitro growth inhibition effect of test compound which was assessed by calorimetric or spectrophotometric method where the yellow-colored MTT converted into purple-colored Formazan crystals by mitochondria in the living cells. Results: Hence, in the results of the current study, mean of five readings was represented. The IC 50 value of TTO for colon cancer cell line after 24 h was 12.5 µg/mL. Spearman's rho's correlation showed P < 0.060 indicating that there were no significant results obtained when TTO was treated with HT29 colon cancer cell line. Conclusion: TTO has a promising anticancer property against colon cancer cell line (HT29) with its IC50 value of 12.5 µg/mL. Hence, this TTO with its greater efficacy related to its anticancer activity can be brought to the level of clinical trials in the coming future.

show abstract

“…Several recent works have presented DMSO as vehicle of different anticancer compounds administered systemically or topically in 1 ~ 10% solution. [68697071]…”

Section: Discussionmentioning

confidence: 99%

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

et al. 2014

View full text Add to dashboard Cite

Background:Pyrostegia venusta (Ker. Gawl.) Miers (Bignoniacea) is a medicinal plant from the Brazilian Cerrado used to treat leucoderma and common diseases of the respiratory system.Objective:To investigate the antitumor activity of P.venusta extracts against melanoma.Materials and Methods:The cytotoxic activity and tumor induced cell death of heptane extract (HE) from P. venusta flowers was evaluated against murine melanoma B16F10-Nex2 cells in vitro and in a syngeneic model in vivo.Results:We found that HE induced apoptosis in melanoma cells by disruption of the mitochondrial membrane potential, induction of reactive oxygen species and late apoptosis evidenced by plasma membrane blebbing, cell shrinkage, chromatin condensation and DNA fragmentation, exposure of phosphatidylserine on the cell surface and activation of caspase-2,-3,-8,-9. HE was also protective against singeneyc subcutaneous melanoma HE compounds were also able to induce cell cycle arrest at G2/M phases on tumor cells. On fractionation of HE in silica gel we isolated a cytotoxic fraction that contained a mixture of saturated hydrocarbons identified by 1H NMR and GC-MS analyses. Predominant species were octacosane (C28H58-36%) and triacontane (C30H62-13%), which individually showed significant cytotoxic activity against murine melanoma B16F10-Nex2 cells in vitro and a very promising antitumor protection against subcutaneous melanoma in vivo.Conclusion:The results suggest that the components of the heptane extract, mainly octasane and triacontane, which showed antitumor properties in experimental melanoma upon regional administration, might also be therapeutic in human cancer, such as in the mostly epidermal and slowly invasive melanomas, such as acral lentiginous melanoma, as an adjuvant treatment to surgical excision.

show abstract

Topically applied Melaleuca alternifolia (tea tree) oil causes direct anti-cancer cytotoxicity in subcutaneous tumour bearing mice

Cited by 29 publications

References 40 publications

Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging

Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging

Evaluation of anticancer activity of Melaleuca alternifolia (i.e., tea tree oil) on colon cancer cell line (HT29) - An in vitro study

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

Contact Info

Product

Resources

About