Topical hesperidin improves epidermal permeability barrier function and epidermal differentiation in normal murine skin

Abstract

Orange peel extract appears to exhibit beneficial effects on skin whitening, inflammation, UVB protection, as well as keratinocyte proliferation. In the present study, we determine whether topical hesperidin influences epidermal permeability barrier function and its underlying mechanisms. Hairless mice were treated topically with 2% hesperidin or 70% ethanol alone twice daily for 6 days. At the end of treatment, basal barrier function as well as transepidermal water loss (TEWL) was measured 2 and 4 hours post barrier disruption. Epidermal proliferation and differentiation were evaluated by immunohistochemical staining and Western blot analysis. Additionally, lamellar body density and secretion were assessed by electron microscopy. Although there were no significant differences in basal barrier function, in comparison to control animals, topical hesperidin significantly accelerated barrier recovery at both 2 and 4 hours after acute barrier abrogation. Enhanced barrier function in hesperidin-treated skin correlated with stimulation of both epidermal proliferation and differentiation, as well as enhanced lamellar body secretion. These results indicate that topical hesperidin enhances epidermal permeability barrier homeostasis at least in part due to stimulation of epidermal proliferation, differentiation, as well as lamellar body secretion.

Topical Apigenin Alleviates Cutaneous Inflammation in Murine Models

“…However, the active constituents of herbal medicines have not yet been well defined. Recently, an active ingredient, hesperidin, in orange peel has been shown to improve the epidermal permeability barrier function [31]. In the present study, we first demonstrated that topical applications of apigenin, an extract from chrysanthemum, inhibit both acute irritant and acute allergic dermatitis in murine models.…”

“…In the case that oxazolone and apigenin or ethanol were applied on the same day, apigenin and ethanol were applied one hour after oxazolone application. On the 8th day, 18 hours after the last apigenin or ethanol application, basal transepidermal water loss (TEWL), stratum corneum hydration, and skin surface pH were measured using their respective probes connected to MPA5 (C&K, Cologne, Germany) as described earlier [31, 32]. …”

Altered sphingoid base profiles predict compromised membrane structure and permeability in atopic dermatitis

“…Recent evidence suggests that AD instead is often initiated by inherited abnormalities that compromise either SC structural or enzymatic proteins [19, 20]. The diminished content of specific species of EOS (Cer 1) and NP (Cer 3) are characteristic downstream features that contribute to the abnormal permeability barriers not only in human [21–23], but also in canine [24] AD.…”

Metotreksatın Neden Olduğu Gastrointestinal Kanaldan Bakteriyel Translokasyonun Önlenmesinde Hesperidinin Etkinliği

“…This is at least partly the result of stimulation of epidermal proliferation and differentiation [25] . Xu et al [26] showed that HES is effective in an experimental study in which they induced experimental colitis with dextran sulphate. We investigated the effect of HES on MTXinduced bacterial translocation in rats.…”