Topical effect of various agents on gelatinase activity in the tear film of normal dogs

Abstract

This study indicated that only EDTA was able to significantly reduce the gelatinase activity in a persistent manner in the tear film of normal canine eyes. Further studies will be required to evaluate the effect of EDTA under ulcerative conditions and to more accurately ascertain the potential in vivo effect of the other agents.

Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury

“…In vivo only EDTA was able to appreciably inhibit gelatinase activity present on normal canine corneal surface (10). This activity is attributed to chelation of Zn, a metal required for MMP activity, by EDTA.…”

Comparison of two methods of tear sampling for protein quantification by Bradford method

“…Researches with tear proteins have always been performed in humans and animals (Ollivier et al 2004, Couture et al 2006), since they provide important information about the course and classification of some diseases and help to find diagnostic and therapeutic targets (Ananthi et al 2008). The Bradford method is suggested as a sensitive method of tear protein quantification (Sitaramamma et al 1998, Ham et al 2007, Ananthi et al 2008), as we observed.…”

“…Lowry (Li et al 2010) and Bradford (Sitaramamma et al 1998, Ananthi et al 2008, Li et al 2010) methods were used to quantify proteins from tear samples (Ananthi et al 2008, Lu et al 2010. For proteomic analysis, tear samples can be obtained from microcapillary tubes (Gachon et al 1982, Jones et al 1997, Couture et al 2006, Grus et al 2007, Green-Church et al 2008 or from Schirmer tear test strips (Grus et al 2007, Li et al 2008, Roberts & Erickson 2008.…”

“…The main focus in clinical proteomics is the discovery of new proteins or peptides that work as biomarkers correlated to a specific disease (Couture et al 2006, Grus et al 2007, Campos et al 2008. Metalloproteinases were identified in the dog (Couture et al 2006) and equine tears from corneal injury (Ollivier et al 2004), and dogs with cancer showed a different expression pattern of proteins (Campos et al 2008). Quantitative determination of tear proteins is of increasing interest in ophthalmology, but remains a technical challenge due to the small sample volumes available and the complexity of its composition (Li et al 2010).…”

A preliminary study of changes in tear film proteins in the feline eye following nictitating membrane removal

“…MMPs, a family of zinc‐ and calcium‐dependent proteinases, are secreted in an inactive state, as pro‐enzymes. Low levels of MMP‐2 and ‐9 (both gelatinases) are detectable in tears collected from healthy horses 14,15 and dogs 16 . The removal of nictitating membranes creates a surgically induced wound that has the potential to involve the expression of MMP‐2 and ‐9 akin to that reported in healing skin wounds 17–19 .…”