Topical aqueous extract of Ephedra alata can improve wound healing in an animal model

Kittana, Naim; Abu-Rass, Hanood; Sabra, Ruba; Manasra, Lama; Hanany, Hadeel; Jaradat, Nidal; Hussein, Fatima; Zaid, Abdel Naser

doi:10.1016/j.cjtee.2016.10.004

Cited by 25 publications

(19 citation statements)

References 13 publications

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“…The E. alata aqueous extract ointment had the good effect on wound healing compared to placebo ointment. In this study the effects of ZOUSH ointment, carvacrol and thymol were the active ingredients of S. khuzestaniea , Z. multiflora , and O. vulgar , and the results showed wound healing in compared with the non-treated group and the group treated with ointment base [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The study of formulated Zoush ointment against wound infection and gene expression of virulence factors Pseudomonas aeruginosa

Meskini

Esmaeili

2018

BMC Complement Altern Med

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BackgroundThe outbreak of MDR and XDR strains of Pseudomonas aeruginosa and increased resistance to infection in burn patients recommend the issue of infection control. In this research, we study ZOUSH herbal ointment for gene silencing of Pseudomonas aeruginosa.MethodsThe herbal ZOUSH ointment was formulated by alcoholic extracts of plants Satureja khuzestaniea, Zataria multiflora, Mentha Mozaffariani Jamzad, honey, and polyurethane. The MIC and disk diffusion tests were examined by single, binary, tertiary and five compounds.Three-week-old mice were considered to be second-degree infections by Pseudomonas aeruginosa. During the interval of 5 days, cultures were done from the liver, blood, and wound by four consecutive quarters and counting of Pseudomonas aeruginosa was reported in the liver.In this study, silver sulfadiazine ointments and Akbar were used as a positive control. The gene gyrA reference was used as the control. Real-time RT-PCR results were evaluated based on Livak as the comparative Ct method.ResultsThe In vitro results indicated that wound infection was improved by healing wound size in the treatment groups compared to control treatment group. In this research, the changes in gene expression were evaluated by molecular technique Real-time RT-PCR. The results showed downregulation exoS, lasA, and lasB after treatment with ZOUSH ointment. SPSS Analyses showed that reduction of expressions in genes exoS, lasA and lasB after treatment with ZOUSH ointment were significantly meaningful (p < 0.05).ConclusionOur study showed that ZOUSH ointment has the positive effect for gene silencing Pseudomonas aeruginosa in the mouse model with the second-degree burn. The positive effects decreased in the number of bacteria by reducing the expression of virulence bacteria genes as exoS, lasA and lasB and improvement of wound healing.

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Section: Discussionmentioning

confidence: 99%

The study of formulated Zoush ointment against wound infection and gene expression of virulence factors Pseudomonas aeruginosa

Meskini

Esmaeili

2018

BMC Complement Altern Med

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“…After this, the mice were killed with excessive anesthesia, and autopsies were collected from the sites of the healing wounds, which were stored in formalin for further histopathological evaluation. 3 All tissue samples were processed and sectioned according to the standard professional procedure followed routinely in the medical laboratory of An-Najah National University Hospital for regular pathological samples. The samples were stained with H&E and Masson's trichrome stain, and then examined by a clinical pathologist who was blinded to the samples.…”

Section: Induction Of Woundsmentioning

confidence: 99%

“…1,2 The development of pharmaceutical preparations that can accelerate the healing process has long been the focus of many research groups and pharmaceutical companies worldwide. 3 Among the many substances investigated for that purpose is a natural polymer called chitosan, which has the advantages of being non-toxic and biocompatible. Chitosan has been incorporated in various topical formulations including powders, hydrogels and dressings, and these preparations have been tested in wound and burn animal models.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan

Kittana¹,

Assali²,

Abu-Rass³

et al. 2018

IJN

Self Cite

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BackgroundImpaired wound healing is commonly associated with many health problems, including diabetes, bedsores and extensive burns. In such cases, healing often takes a long time, which subjects patients to various complications. This study aims to investigate whether single-wall or multi-wall carbon nanotubes complexed with chitosan hydrogel can improve wound healing.Materials and methodsInitially, the effects of the complexes on the viability and functionality of fibroblasts were investigated in engineered connective tissues. Then, their activity on wound healing was investigated in a mouse model with induced full-thickness wounds, in which the wounds were treated daily with these complexes. Finally, the effect of the complexes on collagen deposition by fibroblasts was investigated in vitro.ResultsThe engineered connective tissue studies showed that fibroblasts were viable in the presence of the complexes and were still able to effectively organize and contract the extracellular matrix. In vivo data showed that both types of complexes improved the re-epithelialization of the healing wounds; however, they also increased the percentage of wounds with higher fibrosis. In particular, the chitosan-multi-wall carbon nanotube complex significantly enhanced the extensiveness of this fibrosis, which is in line with in vitro data showing a concentration-dependent enhancement of collage deposition by these complexes. These observations were associated with an increase in inflammatory signs in the wound bed.ConclusionSingle-wall and multi-wall carbon nanotubes complexed with chitosan improved the re-epithelialization of wounds, but an increase in fibrosis was detected.

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“…Among these, the applications of plant based nanotechnology plays a significant role in the process of wound healing [16,17]. The plant based materials have proved at all times to be a powerful remedy for health care [18][19][20][21][22][23][24][25][26][27]. Accordingly, the grape seed extract containing PCs have been investigated for wound treatment and in the management of many illnesses such as cancers, cardiovascular disease, gastric ulcers, obesity and skin inflammations.…”

Section: Introductionmentioning

confidence: 99%

Nano formulated proanthocyanidins as an effective wound healing component

Ramesh

Volova

Oluwafemi

et al. 2020

Materials Science and Engineering: C

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Proanthocyanidins (PCs), a component of grape seed extract (GSE), has recently being used for the treatment of wounds. However, poor absorption, poor stability and rapid elimination from the systemic circulation limit its acceptance. In addressing these problems, we herein report the development of PCs based nanoformulations (PCs/SOLU) for the first time based on 1 % GSE and assessed its wound healing potential in-vivo on the wistar rats. GSE and PCs/SOLU nanodispersions 1 % were prepared by incorporating them into the ointment base via uniform mixing to form ointment which could be easily applied topically to wounds. The antibacterial activity of PCs/SOLU against gram positive and gram-negative bacteria strains proved that the cell membranes became more permeable with disrupted cell structure. While carrageenan and histamine induced rat paw edema analyses show there was no inflammatory signs in animals treated with 1 wt% of PCs/SOLU nanodispersion. Excision wound measuring about 3 cm in depth was created on the wistar rats. The ointment was applied topically on the wounded site and the wound contraction was measured daily. Grape seed extract (GSE) ointment, ointment base and povidone-iodine (Povi-Iod) ointment of about 1 % was used as the control, positive and negative standards. PCs/SOLU nanodispersion heals the wound by mobilising the fibroblasts in the wound site and inhibits the inflammatory response through decreased expression of monocyte. The macroscopical, immunological and histopathological assessments revealed that PCs/SOLU nanodispersion ointment usage improves the cell adhesion and proliferation

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Topical aqueous extract of Ephedra alata can improve wound healing in an animal model

Cited by 25 publications

References 13 publications

The study of formulated Zoush ointment against wound infection and gene expression of virulence factors Pseudomonas aeruginosa

The study of formulated Zoush ointment against wound infection and gene expression of virulence factors Pseudomonas aeruginosa

Enhancement of wound healing by single-wall/multi-wall carbon nanotubes complexed with chitosan

Nano formulated proanthocyanidins as an effective wound healing component

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