Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis

Abstract

KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

Sea Buckthorn (Hippophaë rhamnoides L.) Oil Improves Atopic Dermatitis-Like Skin Lesions via Inhibition of NF-κB and STAT1 Activation

“…Mast cells can release inflammatory mediators to mediate inflammatory responses such as allergic reactions [30,31]. Histologically, there was a high degree of mast cell and inflammatory cell infiltration in the skin tissue of DNCB-sensitized mice, and SBT oil decreased the infiltration of mast cells and inflammatory cells in the skin.…”

Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

“…Another defining characteristic of the allergic immune system is the capacity to generate elevated immunoglobulin E (IgE) antibodies and type 2 helper T cells (Th2), which are critical for IgE synthesis [ 5 ]. The IgE level is associated with the severity of AD, and the abnormal skin barrier in patients with AD, a key feature of the disease, contributes to the increased severity [ 6 ]. The functions of IgE in allergic inflammation suggest that IgE and IgE-mediated activation of mast cells and eosinophils contribute to AD.…”

Cryptotanshinone inhibits IgE‑mediated degranulation through inhibition of spleen tyrosine kinase and tyrosine‑protein kinase phosphorylation in mast cells

“…In most cases, patients with AD are treated with synthetic steroids. However, many clinical reports have warned that long-term use of synthetic steroids may result in side effects such as additional infections, gastrointestinal ulcers, osteoporosis, and insomnia (3)(4)(5). Therefore, the identification of novel anti-allergic naturally-derived agents from herbs and medicinal plants with less side effects are required for AD treatment.…”