Top-Down Processed SOI Nanowire Devices for Biomedical Applications

Ingebrandt, Sven; Vu, Xuan Thang; Eschermann, Jan Felix; Stockmann, Regina; Offenhäusser, Andreas

doi:10.1149/1.3571972

Cited by 18 publications

(10 citation statements)

References 51 publications

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“…These spectra make the devices comparable to other silicon-based transistor devices used earlier in our group (Ingebrandt et al 2011;Susloparova et al 2013). Figure 1 shows the plot of the TC (Fig.…”

Section: Characterization Of the Devicessupporting

confidence: 53%

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Hempel

Law

Nguyen

et al. 2017

Biosensors and Bioelectronics

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Section: Characterization Of the Devicessupporting

confidence: 53%

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Hempel

Law

Nguyen

et al. 2017

Biosensors and Bioelectronics

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“…Glycidoxypropyltrimethoxysilane (GOPMS) with a reactive epoxy end group is used to attach amine-terminated strands of the thrombin-binding aptamer TBA (3 -GGTTGGTGTGGTTGG-T 6 -(CH 2 ) 6 -NH 2 -5 ) via stable amino-alcohol bond formation, as sketched in Figure 2c. GOPMS covalently links to the Al 2 O 3 layer via gas phase adsorption at elevated temperatures as described elsewhere [46]. We confirmed the binding using contact angle measurement (Contact Angle System OCA, DataPhysics, Filderstadt, Germany), dripping a water droplet on the Si-wafer as a control, indicating a homogeneously covered sample with a mean contact angle of 64 degrees compared to 36 degrees for the bare oxide.…”

Section: Methodssupporting

confidence: 60%

Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

et al. 2018

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Abstract:We present a biosensor chip with integrated large area silicon nanowire-based field effect transistors (FET) for human α-thrombin detection and propose to implement the hysteresis width of the FET transfer curve as a reliable parameter to quantify the concentration of biomolecules in the solution. We further compare our results to conventional surface potential based measurements and demonstrate that both parameters distinctly respond at a different analyte concentration range. A combination of the two approaches would provide broader possibilities for detecting biomolecules that are present in a sample with highly variable concentrations, or distinct biomolecules that can be found at very different levels. Finally, we qualitatively discuss the physical and chemical origin of the hysteresis signal and associate it with the polarization of thrombin molecules upon binding to the receptor at the nanowire surface.

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“…At the same time, non-specific adsorption of fully mismatched ssDNAs induces only a small potential shift of less than 5 mV. The hybridization signals detected by the MLAPS functionalized with a PAH/DNA LbL layer were about 5 times higher than that of the capacitive EIS sensor 38 or floating-gate transis- tor structure 47 modified with a poly-L-lysine/DNA LbL layer and comparable to hybridization signals recorded by the MLAPS, 44 capacitive EIS sensors 57 and silicon nanowires 58 with probe ssDNAs covalently attached to the silanized SiO 2 gate surface. In general, the hybridization signal was smaller than the immobilization signal.…”

Section: Label-free Detection Of Dna Hybridizationmentioning

confidence: 60%

Label-free detection of DNA using a light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer

Bronder

Poghossian

et al. 2015

Nanoscale

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A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.

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Top-Down Processed SOI Nanowire Devices for Biomedical Applications

Cited by 18 publications

References 51 publications

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

Label-free detection of DNA using a light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer

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