Toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative for the intraoral reduction of viral load of SARS-CoV-2: a pilot study

Abstract

Background: The aim of this study was to evaluate the effects of the toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative (APD) for the intraoral reduction of viral load of SARS-CoV-2.Material and methods: Twenty COVID-19 positive dentate patients were selected and toothbrushes with a dentifrice containing APD for 2 minutes. Self-collected samples of unstimulated saliva were carried out three times: T0 (baseline, before toothbrushing), T5 (5 minutes after toothbrushing), and T30 (30 minutes after toothbrushing). The analysis of RNA viral was performed by RT-PCR using TaqPath™ COVID-19 multiplex Real-Time RT-PCR test for detection of three viral genes (ORF1ab, N and S genes). Evaluation of the effects was based on difference in cycle threshold (Ct) value. Friedman's test and pairwise comparison with Bonferroni corrections were used, with a significance level of 5%. Results: The Ct values were significantly higher (p=0.020) at T30 in comparison to T0 and T5. The greatest difference in the Ct values was between T30 and T0 (3.83). Conclusion: This pilot study suggests that oral hygiene action associated with an antimicrobial chemical dentifrice may be an important tool for SARS-CoV2 viral load reduction in oral cavity.

Use of Mouthwash and Dentifrice Containing an Antimicrobial Phthalocyanine Derivative for the Reduction of Clinical Symptoms of Covid-19: A Randomized Triple-Blind Clinical Trial

“…The prevalence of symptoms was examined at baseline (T0), 3 (T3), and 7 (T7) days after using APD in oral hygiene products and compared to the control group. These results are in accordance with clinical and laboratory evidence built over this pandemic period on the beneficial effect of using APD in oral care products against COVID-19 in the studies samples 14 , 15 , 16 , 20 , 21 , 22 , 23 .…”

“…In these studies, the patients experienced a reduction in clinical symptoms such as sore throat, cough, and mouth sores and became asymptomatic after a few days of use APD rinse protocol. In addition, a pilot study showed a reduction in the viral load of SARS-CoV-2 after brushing teeth with a dentifrice containing APD 14 , and an in vitro study demonstrated SARS-CoV-2 inactivation on the use of a mouthwash (90%) and dentifrice (99.99%), both containing APD.…”

“…Moreover, during the pandemic, it was hypothesized that these products could be used as adjuvants in the prevention of COVID-19 infection, by reducing the oral viral load of SARS-CoV-2 10 , 11 , 12 . Recent studies have demonstrated promising clinical and laboratory findings such as intraoral reduction of viral load of SARS-CoV-2, virucidal efficacy against salivary SARS-CoV-2, anti-inflammatory effectivity, and possible benefit for COVID-19 with evidence-based approach 10 , 11 , 12 , 13 , 14 , 15 , 16 . In contrast, the daily use of certain oral hygiene virucidal products could cause adverse in patients exposed to long-term povidone iodine (thyroid problems), and chlorhexidine (alteration in taste, staining of teeth, and calculus formation) 17 , 18 .…”

The use of a mouthwash and a dentifrice containing antimicrobial phthalocyanine derivative on the reduction of clinical symptoms of COVID-19: A randomized triple-blinded clinical trial

“…22,23 In these studies the patients had a reduction in clinical symptoms such as sore throat, cough and mouth sores and became asymptomatic after a few days of use. In additional, a pilot study the authors showed reduction of viral load of SARS-CoV-2 after toothbrushing with a dentifrice containing APD 14 and an in vitro study demonstrated SARS-CoV-2 inactivation with a mouthwash (90%) and a dentifrice (99,99%) both containing APD. 19 However, as this is a new technology, there are no results from clinical trials with the APD in patients with COVID-19 during the early stage of the disease.…”

“…[10][11][12] Recently, studies have shown some interesting clinical and laborator. [10][11][12][13][14][15] On the other hand, the daily use of some oral hygiene virucidal products cause adverse effects such as thyroid problems, alteration in taste, staining of teeth, calculus formation, among others. [16][17][18] In this pandemic context, some oral antimicrobial products have been investigated for the maintenance of oral hygiene and as a preventive measure against COVID-19, especially those containing chlorhexidine, hydrogen peroxide, povidone iodine and, more recently, an antimicrobial phthalocyanine derivative (APD).…”