Tooth Slice–Based Models for the Study of Human Dental Pulp Angiogenesis

Gonçalves, Silvana Beltrami; Zhang, Dong; Bramante, Clóvis Monteiro; Holland, G.; Smith, Anthony J.; Nör, Jacques E.

doi:10.1016/j.joen.2007.03.012

Cited by 73 publications

(85 citation statements)

References 30 publications

(28 reference statements)

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“…VegfA (vascular endothelial cell growth factor A), a Bmp2-regulated protein, is a key factor in angiogenesis and blood vessel formation in bone marrow osteoblasts and dental pulp region (Deckers et al, 2002;Gonçalves et al, 2007;Zhang et al, 2009;Maes et al, 2010b). Bmp2 has been shown to promote proliferation and migration of endothelial cells through the VegfA/VegfR2 signaling pathway (Suzuki et al, 2008).…”

Section: Tissue Preparation and Analysismentioning

confidence: 99%

Bmp2 Is Required for Odontoblast Differentiation and Pulp Vasculogenesis

et al. 2011

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrActUsing the Bmp2 floxed/3.6Col1a1-Cre (Bmp2-cKO od ) mouse model, we have observed severe defects in odontogenesis and dentin formation with the removal of the Bmp2 gene in early-polarizing odontoblasts. The odontoblasts in the Bmp2-cKO od do not mature properly and fail to form proper dentin with normal dentinal tubules and activate terminal differentiation, as reflected by decreased Osterix, Col1a1, and Dspp expression. There is less dentin, and the dentin is hypomineralized and patchy. We also describe an indirect effect of the Bmp2 gene in odontoblasts on formation of the vascular bed and associated pericytes in the pulp. This vascular niche and numbers of CD146+ pericytes are likely controlled by odontogenic and Bmp2-dependent VegfA production in odontoblasts. The complex roles of Bmp2, postulated to be both direct and indirect, lead to permanent defects in the teeth throughout life, and result in teeth with low quantities of dentin and dentin of poor quality.

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Section: Tissue Preparation and Analysismentioning

confidence: 99%

Bmp2 Is Required for Odontoblast Differentiation and Pulp Vasculogenesis

et al. 2011

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“…Several in vivo study models have been developed and conducted using many cell lines 1,2,36,38,40 . Synthetic and natural scaffolds have been developed and applied successfully 16,24,26 . Moreover, several techniques used in modern clinical dentistry emerged from the application of tissue engineering principles.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, its use in scaffolds may increase blood vessel anastomosis in addition to microvessel density, thereby providing proper nutrition for the establishment of the cell/scaffold assembly 16 . Mullane et al 17 tested different concentrations of VEGF and fibroblast growth factor-2 (FGF-2) and observed in vivo that, after fourteen days, the treatment with 50 ng/mL of VEGF was superior to the other groups and to the groups not treated with growth factors.…”

Section: Bioactive Moleculesmentioning

confidence: 99%

“…Gonçalves et al 16 used the tooth slice/scaffold (TS/S) model to evaluate the angiogenesis provided by VEGF in pulp-like tissues. TS/S is an in vivo model where horizontal sections in the region of the cement/enamel junction are performed, so the tissue that is present is removed.…”

Section: Scaffolds In Regenerative Therapiesmentioning

confidence: 99%

“…In addition, the tooth slice obtained is filled by PLLA scaffolds and these are seeded with stem cells. The TS/S assembly is thus implanted on the dorsum of immunosuppressed mice, where it is possible to evaluate the interaction of the TS/S with the cells of the animal organism 16 .…”

Section: Scaffolds In Regenerative Therapiesmentioning

confidence: 99%

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What is the clinical applicability of regenerative therapies in dentistry?

Demarco

Kirschnick

Watson

et al. 2017

RGO, Rev. Gaúch. Odontol.

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Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists' knowledge of these new therapies RESUMOTerapias regenerativas vem sendo amplamente desenvolvidas na odontologia e o conhecimento destas novas terapias por parte dos dentistas é importante para que elas sejam incorporadas na rotina clínica odontológica. Assim, este estudo revisou a literatura acerca das terapias regenerativas e suas aplicações clínicas. A engenharia tecidual tem contribuído na mudança do paradigma restaurador das ciências da saúde. Seus pilares embasam as técnicas de regeneração de tecidos e órgãos. Apesar da grande maioria dos estudos neste campo ser in vitro, uma gama de metodologias pré-clínicas foi consolidada e estudos utilizando estes princípios já estão sendo empregados em humanos. A utilização de plasma rico em plaquetas e plasma rico em fibrina como scaffolds naturais em cirurgias para reestabelecimento de tecido ósseo e periodontal são frequentemente relatadas na literatura e ensaios clínicos utilizando esta abordagem demonstram resultados promissores. Células-tronco da polpa dental autólogas já foram aplicadas com sucesso na regeneração de tecido ósseo utilizando scaffold naturais de colágeno em humanos. Além disto, a revascularização do canal radicular já aparece na literatura como uma alternativa promissora frente a opção de apecificação do canal radicular. Esta terapia utiliza como princípio o coágulo sanguíneo como scaffold e a migração das células-tronco da papila apical para regenerar o órgão pulpar. Considerações finais: Apesar de incipientes, as terapias regenerativas já podem ser utilizadas na prática clínica odontológica. O conhecimento dos princípios que regem estas terapias deve ser compreendido pelo dentista para que sejam utilizadas na prática clínica.Termos de indexação: Fibrina. Plasma rico em plaquetas. Células-tronco. Engenharia tecidual.

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