The aim of this study was to determine the action of desensitizing materials on bovine dentin. Four extracted healthy bovine molars were selected out of which 5 blocks from cervical region of each tooth were obtained, resulting in a 5x5mm final area. To compose the negative control group (group B), the samples were separated and not submitted to any treatment. For the positive control (Group A) the blocks were treated with 37% phosphoric acid. For allow to use the same dentin samples for all groups (2 controls and 3 test group), the experiment group contained twelve dentin surface blocks divided in 5 groups (n=4 in each group), as manufacturers' recommendations, there were used: Dessensibilize KF 2%-5% Potassium nitrate and 2% sodium fluoride (C); Fluoride Gel-2% sodium fluoride (D), Colgate Sensitive Pró-Alívio ® Arginine 8% (E) and Calcium carbonate-based toothpaste (F). The samples were analyzed in Scanning Electron Microscopy (SEM) and used the ANOVA and Tukey tests (p<005). The number and diameters of dentinal tubules were compared across the various groups. The samples from group E had a higher quantity of deposits on the peritubular and intertubular dentin with most closed tubules. Although, the groups A and B presented more opened dentinal tubules. However, only A has deposited a substratum from the reaction with desensitizing materials on the peritubular and intertubular dentin indicating same closed tubules. The analysis of the diameters of the tubules showed significant differences in groups B and C when compared to the other groups. Research suggests that arginine, a natural amino acid found on the saliva, together with calcium carbonate, positively charged at physiological pH, are bound to the dentin surface, negatively charged, and help forming a calcium-rich layer on the dentin surface and within the dentinal tubules, sealing them. The arginine causes the occlusion of the dentinal tubules and, theoretically, such occlusion remains intact even after exposure to acids [10]. Therefore, the purpose of this study was to test, in vitro, the action of several desensitizing materials in bovine dentin, verifying its effectiveness in obliterating the dentinal tubules. Material and Methods The study were laboratorial, in vitro one with convenience sample. The products used were select among the most used by dental sensitivity and the bovine dentin by human similarity. Material selection Three products for professional use with a known desensitizing action have been tested. Product 1: Dessensibilize KF 2%-5% Potassium nitrate and 2% sodium fluoride (FGM) Product 2: Flutop Gel Neutro-2% sodium fluoride (SSWhite) Product 3: Colgate Sensitive Pró-Alívio ® Arginine-(8%) and Calcium carbonate-based toothpaste (Colgate) Sample preparation and experimentation This study used three experimental group and two controls (positive and negative one). The objective were the dentin substrate of the same tooth had be used in all groups to avoid a sample bias,