Tooth-derived bone graft material

Abstract

With successful extraction of growth factors and bone morphogenic proteins (BMPs) from mammalian teeth, many researchers have supported development of a bone substitute using tooth-derived substances. Some studies have also expanded the potential use of teeth as a carrier for growth factors and stem cells. A broad overview of the published findings with regard to tooth-derived regenerative tissue engineering technique is outlined. Considering more than 100 published papers, our team has developed the protocols and techniques for processing of bone graft material using extracted teeth. Based on current studies and studies that will be needed in the future, we can anticipate development of scaffolds, homogenous and xenogenous tooth bone grafts, and dental restorative materials using extracted teeth.

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

“…It is known that the composition of DDM is similar to that of the autogenous bone in terms of the proportion of inorganic (65%) and organic (35%) substances 6,7) . Experiments, such as the Bradford assay, for assessing the organic composition of DDM suggested that the osteoinductivity of DDM was sufficient for new bone formation 12,34) . Among the organic components, DDM is composed at 90% of type I collagen and 10% of noncollagenous proteins, including osteocalcin, osteopontin, osterix, Runx2, phosphophoryn, and sialoprotein 34) , which are less represented in Bio-Oss Collagen ® and could partially explain the higher osteogenic potential of the DDM in our study.…”

“…Since the development of tooth-derived bone grafting materials, reports showed that the physicochemical characteristics of autogenous demineralized dentin matrix (DDM) were similar to those of autogenous cortical bone [5][6][7][8] . Owing to this advantage, bone grafting materials from the autogenous tooth have been used actively, and their clinical applications were approved 6,[9][10][11][12][13][14][15][16] . Recently, a 5-year-long followup of DDM based on cone-beam computed tomography evaluation showed s uccessful guided bo n e regen erati on with ou t complications 17) .…”

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

“…In 2008, an autogenous bone graft material was developed from extracted teeth, and subjected to many basic and clinical studies. It has since been found to be an excellent biocompatible material with osteoinductive and osteoconductive potential [5][6][7][8]15,16) . Researches that demineralized dentin matrix (DDM) can play a role as the scaffold of bone morphogenic protein (BMP) and growth factors were announced.…”

“…The clinical effectiveness of autogenous tooth bone graft, which was domestically developed in 2008 and now being actively used, was already proved. Also, it was reported that it showed the similar healing state to free autogenous bone graft as its osteoconductive and osteoinductive healing were excellent and bone remodeling was good [5][6][7] . Meanwhile, although tissue engineering research using various growth factors, bone morphogenic protein, and stem cell, etc is actively being performed worldwide, the appropriate scaffold to carry them have not been developed much.…”

Dental Stem Cells Characterization and Bone Regenerative Potential in Oral Medicine

“…Bone morphogenic proteins have long been utilized in scaffolds as stimulants for a hastened growth of bone matrix and cartilage [40]. Presently, the transfection of genetic factors to cellular populations in scaffolds, namely PDLSCs, resulted in an escalation of PDGF, with greater cementoblastic activity than reported in recombinant PDGF delivery [41].…”